A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen ...A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.展开更多
Objective: To seek new method for the treatment of peripheral nerve injury. Methods: In rat model with sciatic nerve defect, chitosan collagen film was sutured into conduit to bridge 5 mm , 10 mm nerve defects. Rats t...Objective: To seek new method for the treatment of peripheral nerve injury. Methods: In rat model with sciatic nerve defect, chitosan collagen film was sutured into conduit to bridge 5 mm , 10 mm nerve defects. Rats that underwent end to end anastomosis were taken as controls. General observation, electrophysiological study, histological study and image analysis were performed at 4, 8, 12 weeks postoperatively. Results: In 5 mm nerve defects, the quality of nerve regeneration was similar to that of the control group. For 10 mm nerve defect, nerve regeneration was inferior to that of the control group. Chitosan collagen film obviously degraded at 12 weeks postoperatively. Conclusions: Chitosan collagen film conduit can be used to bridge peripheral nerve defect.展开更多
To study the proliferation and collagen production of tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes; and the effects of chitosan on cell proliferation and collagen production in the 3 cell typ...To study the proliferation and collagen production of tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes; and the effects of chitosan on cell proliferation and collagen production in the 3 cell types of rabbit flexor tendon. Methods ： Three cell lines of tendon sheath, epitenon, and endotenon were isolated from rabbit flexor tendon and cultured. Cell culture media was added with chitosan. The cell number and production of collagens Ⅰ, Ⅱ, and Ⅲ were measured and compared with those cultured without chitosan. The expression of type I collagen in tendon sheath fibroblasts was determined by quantitative analysis of reverse-transcription polymerase chain reaction. Results ： All 3 cell lines produced collagens Ⅰ, Ⅱ, and Ⅲ. Adding chitosan to cell media resulted in a significant decrease in cell number in all 3 cell lines. In addition, there was a significant decrease in collagens Ⅰ, Ⅱ, and Ⅲ production in all 3 cell lines as well as the expression levels of type I collagen in tendon sheath fibroblasts （ P 〈 0.05 ）. Conclusions： Chitosan can inhibit cell proliferation and collagen production of the tendon sheath, epitenon, and endotenon, and may provide a promising approach to obviating tendon adhesion formation clinically.展开更多
文摘A novel biomaterial scaffold was created from collagen chitosan/GAG. Its tensile strength was 8.6MPa(wet state)and degree of swelling water was 60%～75% with higer ultimate elongation 300%. Rabbit corneas of collagen chitosan/GAG implantation samples in vivo for biodegradation showed that the inplantion samples was complets biodegrable and digested afere 120 day. There was enought time to maintain cell growth,immigrating and proliferation. This biomaterials scaffold can be used for cell culture and in various tissue engineering fields.
文摘Objective: To seek new method for the treatment of peripheral nerve injury. Methods: In rat model with sciatic nerve defect, chitosan collagen film was sutured into conduit to bridge 5 mm , 10 mm nerve defects. Rats that underwent end to end anastomosis were taken as controls. General observation, electrophysiological study, histological study and image analysis were performed at 4, 8, 12 weeks postoperatively. Results: In 5 mm nerve defects, the quality of nerve regeneration was similar to that of the control group. For 10 mm nerve defect, nerve regeneration was inferior to that of the control group. Chitosan collagen film obviously degraded at 12 weeks postoperatively. Conclusions: Chitosan collagen film conduit can be used to bridge peripheral nerve defect.
文摘To study the proliferation and collagen production of tendon sheath fibroblasts, epitenon tenocytes, and endotenon tenocytes; and the effects of chitosan on cell proliferation and collagen production in the 3 cell types of rabbit flexor tendon. Methods ： Three cell lines of tendon sheath, epitenon, and endotenon were isolated from rabbit flexor tendon and cultured. Cell culture media was added with chitosan. The cell number and production of collagens Ⅰ, Ⅱ, and Ⅲ were measured and compared with those cultured without chitosan. The expression of type I collagen in tendon sheath fibroblasts was determined by quantitative analysis of reverse-transcription polymerase chain reaction. Results ： All 3 cell lines produced collagens Ⅰ, Ⅱ, and Ⅲ. Adding chitosan to cell media resulted in a significant decrease in cell number in all 3 cell lines. In addition, there was a significant decrease in collagens Ⅰ, Ⅱ, and Ⅲ production in all 3 cell lines as well as the expression levels of type I collagen in tendon sheath fibroblasts （ P 〈 0.05 ）. Conclusions： Chitosan can inhibit cell proliferation and collagen production of the tendon sheath, epitenon, and endotenon, and may provide a promising approach to obviating tendon adhesion formation clinically.
