Bergmann glia cells are a discrete radial glia population surrounding Purkinje cells in the cerebellar cortex. Although Bergmann glia are essential for the development and correct arborization of Purkinje cells, littl...Bergmann glia cells are a discrete radial glia population surrounding Purkinje cells in the cerebellar cortex. Although Bergmann glia are essential for the development and correct arborization of Purkinje cells, little is known about the regulation of this cell population after the developmental phase. In an effort to characterize this population at the molecular level, we have analyzed marker expression and established that adult Bergmann glia express Soxl, Sox2 and Sox9, a feature otherwise associated with neural stem cells （NSCs）. In the present study, we have further analyzed the developmental pattern of Soxl-expressing cells in the developing cerebellum. We report that before becoming restricted to the Purkinje cell layer, Soxl-positive cells are present throughout the immature tissue, and that these cells show characteristics of Bergmann glia progenitors. Our study shows that these progenitors express Soxl, Sox2 and Sox9, a signature maintained throughout cerebellar maturation into adulthood. When isolated in culture, the Soxl-expressing cerebellar population exhibited neurosphere-forming ability, NSC-marker characteristics, and demonstrated multipotency at the clonal level. Our results show that the Bergmann glia population expresses Soxl during cerebellar development, and that these cells can be isolated and show stem cell characteristics in vitro, suggesting that they could hold a broader potential than previously thought.展开更多
The solution of vibration transfer from the subsoil to the structure is demonstrated using the example of a multi-storey reinforced concrete building, founded on a dual foundation plate. An anti-vibration layer of rub...The solution of vibration transfer from the subsoil to the structure is demonstrated using the example of a multi-storey reinforced concrete building, founded on a dual foundation plate. An anti-vibration layer of rubber has been designed between the two plates. Two 3D numerical models of the building take into account the individual storeys, firstly together with the lay-out of the rubber distribution in the foundation pan and secondly without this rubber part. For response analysis, the measured time histories in the construction area were selected and then the typical response was used as an input for a dynamic analysis of the structure.展开更多
文摘Bergmann glia cells are a discrete radial glia population surrounding Purkinje cells in the cerebellar cortex. Although Bergmann glia are essential for the development and correct arborization of Purkinje cells, little is known about the regulation of this cell population after the developmental phase. In an effort to characterize this population at the molecular level, we have analyzed marker expression and established that adult Bergmann glia express Soxl, Sox2 and Sox9, a feature otherwise associated with neural stem cells （NSCs）. In the present study, we have further analyzed the developmental pattern of Soxl-expressing cells in the developing cerebellum. We report that before becoming restricted to the Purkinje cell layer, Soxl-positive cells are present throughout the immature tissue, and that these cells show characteristics of Bergmann glia progenitors. Our study shows that these progenitors express Soxl, Sox2 and Sox9, a signature maintained throughout cerebellar maturation into adulthood. When isolated in culture, the Soxl-expressing cerebellar population exhibited neurosphere-forming ability, NSC-marker characteristics, and demonstrated multipotency at the clonal level. Our results show that the Bergmann glia population expresses Soxl during cerebellar development, and that these cells can be isolated and show stem cell characteristics in vitro, suggesting that they could hold a broader potential than previously thought.
文摘The solution of vibration transfer from the subsoil to the structure is demonstrated using the example of a multi-storey reinforced concrete building, founded on a dual foundation plate. An anti-vibration layer of rubber has been designed between the two plates. Two 3D numerical models of the building take into account the individual storeys, firstly together with the lay-out of the rubber distribution in the foundation pan and secondly without this rubber part. For response analysis, the measured time histories in the construction area were selected and then the typical response was used as an input for a dynamic analysis of the structure.
