为解决通过卷烟外包装、烟支物化参数等进行卷烟真伪鉴定难度大且耗时费力等问题,提出了一种基于卷烟小盒内部粘胶痕迹形态差异的卷烟真伪鉴别方法。首先利用胶痕图像采集装置获取烟盒内部的胶痕图像;然后去除原始图像的部分背景得到胶...为解决通过卷烟外包装、烟支物化参数等进行卷烟真伪鉴定难度大且耗时费力等问题,提出了一种基于卷烟小盒内部粘胶痕迹形态差异的卷烟真伪鉴别方法。首先利用胶痕图像采集装置获取烟盒内部的胶痕图像;然后去除原始图像的部分背景得到胶痕图像样本;基于深度学习异常检测方法,仅使用正常样本训练卷积神经网络分类模型,再通过模型对待测样本产生的最大预测概率进行阈值判别。以10种包装机型生产的真品烟盒以及假冒烟盒(烟盒样品涉及约60个卷烟规格)为对象,分别选用5个经典的卷积神经网络模型进行实验,并对1294个待测样本进行鉴别。结果表明:(1)当使用测试集样本的最大预测概率平均值作为阈值时,所有模型均具备一定的真伪判别能力。(2)ResNet18、DenseNet121、InceptionV1的鉴别准确率约为94%,召回率约为93%~94%,特异性约为93%~95%,AUC(Area Under Curve)约为0.98;InceptionV1参数量较少,易于部署。(3)本方法能够在完全不使用假样本的情况下构建鉴别模型,且适用于多种卷烟规格。该技术可为建立高效、可靠的卷烟真伪智能鉴别方法提供支持。展开更多
Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblas...Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression, mRNA expression of collagen Ⅰ and Ⅲ, and transforming growth factor ( TGF)-β1 were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Results Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent man- ner. Immunocytochemical staining indicated that collagenⅠ and Ⅲ were down-regulated by quercetin and X-ray (P 〈 0.05 ), particularly collagen I ( P 〈 0. 05 ). mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group ( P 〈 0. 05 ), especially in the group treated with both quercetin and X-ray ( P 〈 0. 01 ). mRNA level of TGF-[31 gene was down-regulated by quercertin ( P 〈 0. 05 ). Conclusions Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.展开更多
Objective As the beneficial effect to the skin scar under external bandage compression, intra-choledocal stent must have the same effect on splanchnic scar formation. The experiment consists to work out the time optim...Objective As the beneficial effect to the skin scar under external bandage compression, intra-choledocal stent must have the same effect on splanchnic scar formation. The experiment consists to work out the time optimum to yield a minimum scar formation. Methods By means of transmitting electronic microscope (TEM), computer assisted three-dimensional morphometry (CAM), and biochemical analysis to determine the extracellular collagen volume density (ECVD) and biochemical collagen content (BCC), to analyze the ultrastructure and components within scar tissues removed from the specimens in 3 groups of experimental animals were detailed. Results In the animals of simple choledoco-jejunostomy (CJ) group, active scar proliferation was seen in all specimens excised within one year after operation. In the stent group, decreasing collagen fibers arranged in orientation began to appear in the 6-month specimens and scar maturation existed in the 9- and 12-month specimens. In periodic tube withdrawal group, 3 months following tube ablation, scar proliferation recurred in the 6th month tube retaining animals, whereas scar maturation without recurrence happened in animals following 9 to 12 months tube retaining. Conclusion 9~12 months of tube stent is necessary for stable scar maturation.展开更多
Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encap...Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblasts (HKFs) culture media. The intracellular distribution of CTGF ASODN was observed by fluorescence microscopy in the fixed HKFs. The proliferation of HKFs was measured by MTT test. The collagen synthesis of HKFs was measured by 3H-proline incorporation method. Results: Compared with control group, the CTGF ASODN can inhibit the proliferation and collagen synthesis of the HKFs (P<0.01). Conclusion: CTGF ASODN has anti-fibrotic effects on keloid in vitro, and CTGF play an important role in promoting the fibrosis of keloid.展开更多
Objective: To determine the efficacy of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits. Methods: Twenty-four Japanese white rabbits underwent laminectomy (including the atta...Objective: To determine the efficacy of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits. Methods: Twenty-four Japanese white rabbits underwent laminectomy (including the attached ligaments) at Lz and Ls. After laminectomy at Ls, polylactic acid glue was sprayed on the dura and nerve roots and this segment was taken as the experimental group. After laminectomy at Lz, nothing was used and this segment was enrolled as the self control group. Four rabbits were killed every two weeks postoperatively till the end of the experiment at 12 weeks. Then the operated spine was observed grossly, histologically and ultrastructurally to check the degree of scar formation, the status of epidural scar adhesion, the absorption of the glue, and the intraceHular structure of fibroblasts. Results: The glue coagulated immediately after spraying and showed excellent hemostatic effect. The glue membrane was easy to be taken away from the dura mater of the samples for 2 weeks and there were no cells in the epidural space in the experimental group. But the dura mater was covered by hematoma in the control group,which formed mild adhesion, with fibroblasts proliferating actively. In the 4th week, some glue shivers remained in the epidural space with fibroblasts increasing a little, and the dura mater was smooth in the experimental group. However, in the control group, the formed scar was fragile and conglutinated with the dura mater diffusely and fibroblasts were much more than those in the experimental group. In the 6th-12th weeks, there was a potential interspace between the scar and the dura mater, and the polylactic acid glue was absorbed completely in the experimental group. Much tough scar was found in the control group, which was very difficult to dissect from the dura mater and the surrounding tissues. From the ultrastructural observation of the fibroblasts, the nucleus became much bigger and the rough endoplasmic reticulum was much more plentiful in the control group than that in the experimental group. Conclusions: Polylactic acid glue can effectively reduce epidural cicatrization and adhesion.展开更多
文摘为解决通过卷烟外包装、烟支物化参数等进行卷烟真伪鉴定难度大且耗时费力等问题,提出了一种基于卷烟小盒内部粘胶痕迹形态差异的卷烟真伪鉴别方法。首先利用胶痕图像采集装置获取烟盒内部的胶痕图像;然后去除原始图像的部分背景得到胶痕图像样本;基于深度学习异常检测方法,仅使用正常样本训练卷积神经网络分类模型,再通过模型对待测样本产生的最大预测概率进行阈值判别。以10种包装机型生产的真品烟盒以及假冒烟盒(烟盒样品涉及约60个卷烟规格)为对象,分别选用5个经典的卷积神经网络模型进行实验,并对1294个待测样本进行鉴别。结果表明:(1)当使用测试集样本的最大预测概率平均值作为阈值时,所有模型均具备一定的真伪判别能力。(2)ResNet18、DenseNet121、InceptionV1的鉴别准确率约为94%,召回率约为93%~94%,特异性约为93%~95%,AUC(Area Under Curve)约为0.98;InceptionV1参数量较少,易于部署。(3)本方法能够在完全不使用假样本的情况下构建鉴别模型,且适用于多种卷烟规格。该技术可为建立高效、可靠的卷烟真伪智能鉴别方法提供支持。
文摘Objoctive To investigate the effects of quercetin and X-ray on collagen synthesis of cultured human keloid-derived fibroblast and the mechanism. Methods Collagen synthesis of cultured human keloid and normal fibroblasts were detected by hydroxyproline colorimetric analysis. Immunocytochemical staining was used to investigate collagen I and III expression, mRNA expression of collagen Ⅰ and Ⅲ, and transforming growth factor ( TGF)-β1 were assayed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Results Quercetin inhibited the collagen synthesis of both keloid and normal fibroblasts in a dose-dependent man- ner. Immunocytochemical staining indicated that collagenⅠ and Ⅲ were down-regulated by quercetin and X-ray (P 〈 0.05 ), particularly collagen I ( P 〈 0. 05 ). mRNA expression of both collagen I and III in quercetin groups significantly decreased compared with that in control group ( P 〈 0. 05 ), especially in the group treated with both quercetin and X-ray ( P 〈 0. 01 ). mRNA level of TGF-[31 gene was down-regulated by quercertin ( P 〈 0. 05 ). Conclusions Quercetin will probably be one of the new medicines which could effectively treat keloid. Quercetin combined with X-ray could reduce the dose of radiation.
文摘Objective As the beneficial effect to the skin scar under external bandage compression, intra-choledocal stent must have the same effect on splanchnic scar formation. The experiment consists to work out the time optimum to yield a minimum scar formation. Methods By means of transmitting electronic microscope (TEM), computer assisted three-dimensional morphometry (CAM), and biochemical analysis to determine the extracellular collagen volume density (ECVD) and biochemical collagen content (BCC), to analyze the ultrastructure and components within scar tissues removed from the specimens in 3 groups of experimental animals were detailed. Results In the animals of simple choledoco-jejunostomy (CJ) group, active scar proliferation was seen in all specimens excised within one year after operation. In the stent group, decreasing collagen fibers arranged in orientation began to appear in the 6-month specimens and scar maturation existed in the 9- and 12-month specimens. In periodic tube withdrawal group, 3 months following tube ablation, scar proliferation recurred in the 6th month tube retaining animals, whereas scar maturation without recurrence happened in animals following 9 to 12 months tube retaining. Conclusion 9~12 months of tube stent is necessary for stable scar maturation.
文摘Objective: To explore the effects of connective tissue growth factor (CTGF) on the pathogenesis of human keloid. Methods: CTGF antisense oligonucleotides (ASODN) conjugated with isothiocyananate fluorescence was encapsulated by liposome, and then added into the human keloid fibroblasts (HKFs) culture media. The intracellular distribution of CTGF ASODN was observed by fluorescence microscopy in the fixed HKFs. The proliferation of HKFs was measured by MTT test. The collagen synthesis of HKFs was measured by 3H-proline incorporation method. Results: Compared with control group, the CTGF ASODN can inhibit the proliferation and collagen synthesis of the HKFs (P<0.01). Conclusion: CTGF ASODN has anti-fibrotic effects on keloid in vitro, and CTGF play an important role in promoting the fibrosis of keloid.
文摘Objective: To determine the efficacy of polylactic acid glue in preventing epidural scar adhesion after laminectomy in rabbits. Methods: Twenty-four Japanese white rabbits underwent laminectomy (including the attached ligaments) at Lz and Ls. After laminectomy at Ls, polylactic acid glue was sprayed on the dura and nerve roots and this segment was taken as the experimental group. After laminectomy at Lz, nothing was used and this segment was enrolled as the self control group. Four rabbits were killed every two weeks postoperatively till the end of the experiment at 12 weeks. Then the operated spine was observed grossly, histologically and ultrastructurally to check the degree of scar formation, the status of epidural scar adhesion, the absorption of the glue, and the intraceHular structure of fibroblasts. Results: The glue coagulated immediately after spraying and showed excellent hemostatic effect. The glue membrane was easy to be taken away from the dura mater of the samples for 2 weeks and there were no cells in the epidural space in the experimental group. But the dura mater was covered by hematoma in the control group,which formed mild adhesion, with fibroblasts proliferating actively. In the 4th week, some glue shivers remained in the epidural space with fibroblasts increasing a little, and the dura mater was smooth in the experimental group. However, in the control group, the formed scar was fragile and conglutinated with the dura mater diffusely and fibroblasts were much more than those in the experimental group. In the 6th-12th weeks, there was a potential interspace between the scar and the dura mater, and the polylactic acid glue was absorbed completely in the experimental group. Much tough scar was found in the control group, which was very difficult to dissect from the dura mater and the surrounding tissues. From the ultrastructural observation of the fibroblasts, the nucleus became much bigger and the rough endoplasmic reticulum was much more plentiful in the control group than that in the experimental group. Conclusions: Polylactic acid glue can effectively reduce epidural cicatrization and adhesion.