针对在复杂光照条件下线胶质量检测存在自动化程度不高,检测精度低等问题,提出了一种基于最小二乘拟合的线胶质量检测方法。首先使用非局部均值滤波降噪算法对图像进行降噪处理;随后通过动态阈值分割法确定待检测线胶区域,并在待检测区...针对在复杂光照条件下线胶质量检测存在自动化程度不高,检测精度低等问题,提出了一种基于最小二乘拟合的线胶质量检测方法。首先使用非局部均值滤波降噪算法对图像进行降噪处理;随后通过动态阈值分割法确定待检测线胶区域,并在待检测区域内使用基于形态学的laplacian of gaussian边缘提取算法提取线胶轮廓;最后使用基于最小二乘拟合的线胶边缘轮廓拟合算法,计算拟合直线之间的平均距离即可得到线胶宽度;将线胶宽度信息与设定胶宽进行对比,判断是否存在断胶等质量问题。经实验验证,在复杂的光照条件下,该方法能准确检测出线胶宽度信息,满足对工业生产线的在线检测要求,其检测高效快速,准确率较高,能有效减少人力与资金投入,适合在实际生产中应用。展开更多
Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy (SBCE) sys- te...Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy (SBCE) sys- tems (PillCam and MiroCam) was performed. The oral cavity/aero-digestive tract (i.e., tongue, uvula and/or epiglottis) was captured/identified in almost all (99%) of PillCam videos but in none of MiroCam cases, P 〈 0.0001. Furthermore, oesophageal images (i.e., from the upper oesophageal sphincter to the Z-line were cap- tured in 99% of PillCam videos (mean =1= SD, 60.5 ± 334.1 frames, range: 0-3329 frames) and in 66% of Mi- roCam cases (mean ± SD, 11.1 ± 46.5 frames, range: 0-382 frames), P 〈 0.0001. The Z-line was identified in 42% of PilICam videos and 17% of MiroCam, P = 0.0002. This information might be useful when perform- ing SBCE in patients with high risks for aspiration.展开更多
AIM:To detect the prevalence of small bowel polyps by wireless capsule endoscopy(WCE)in patients with familial adenomatous polyposis(FAP).METHODS:We examined prospectively 14 patients with FAP to assess the location,s...AIM:To detect the prevalence of small bowel polyps by wireless capsule endoscopy(WCE)in patients with familial adenomatous polyposis(FAP).METHODS:We examined prospectively 14 patients with FAP to assess the location,size and number of small-intestinal polyps.Patients'age,sex,years of observation after surgery,type of surgery,duodenal polyps and colorectal cancer at surgery were analyzed.RESULTS:During WCE,polyps were detected in 9/14(64.3%)patients.Duodenal adenomatous polyps were found in nine(64.3%)patients,and jejunal and ileal polyps in seven(50%)and eight(57.1%),respectively.The Spigelman stage of duodenal polyposis was associated with the presence of jejunal and ileal polyps.Identification of the ampulla of Vater was not achieved with WCE.Importantly,the findings of WCE had no immediate impact on the further clinical management of FAP patients.No procedure-related complications were observed in the patients.CONCLUSION:WCE is a promising noninvasive new method for the detection of small-intestinal polyps.Further investigation is required to determine which phenotype of FAP is needed for surveillance with WCE.展开更多
A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-termi...A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-terminal domain of β-lactam sensor-transducer mutant from Bacillus.licheniformis, served as the recognition element. Colloidal gold(CG)-labelled receptor antibody-receptor ternary complex was applied as a signal transducer probe. The presence ofβ-lactams was identified through a color change in the test zone, where the reaction between the complex of CG/antireceptor antibody/receptor and β-lactam antigen on the test line could be inhibited by β-lactam residues in the sample,thus leading to a reduction in color signal. Based on the indirect labeling of the receptor with an antibody as a linker, the ability to detect 33 β-lactams was below or near the corresponding maximum residue limit. The proposed lateral flow immunoassay biosensor appears to be an excellent field-based screening tool for the qualitative screening of β-lactams in milk.展开更多
文摘针对在复杂光照条件下线胶质量检测存在自动化程度不高,检测精度低等问题,提出了一种基于最小二乘拟合的线胶质量检测方法。首先使用非局部均值滤波降噪算法对图像进行降噪处理;随后通过动态阈值分割法确定待检测线胶区域,并在待检测区域内使用基于形态学的laplacian of gaussian边缘提取算法提取线胶轮廓;最后使用基于最小二乘拟合的线胶边缘轮廓拟合算法,计算拟合直线之间的平均距离即可得到线胶宽度;将线胶宽度信息与设定胶宽进行对比,判断是否存在断胶等质量问题。经实验验证,在复杂的光照条件下,该方法能准确检测出线胶宽度信息,满足对工业生产线的在线检测要求,其检测高效快速,准确率较高,能有效减少人力与资金投入,适合在实际生产中应用。
文摘Transmission of oesophageal images may vary between different small-bowel capsule endoscopy models. A retrospective review of 100 examinations performed with 2 different Small-bowel capsule endoscopy (SBCE) sys- tems (PillCam and MiroCam) was performed. The oral cavity/aero-digestive tract (i.e., tongue, uvula and/or epiglottis) was captured/identified in almost all (99%) of PillCam videos but in none of MiroCam cases, P 〈 0.0001. Furthermore, oesophageal images (i.e., from the upper oesophageal sphincter to the Z-line were cap- tured in 99% of PillCam videos (mean =1= SD, 60.5 ± 334.1 frames, range: 0-3329 frames) and in 66% of Mi- roCam cases (mean ± SD, 11.1 ± 46.5 frames, range: 0-382 frames), P 〈 0.0001. The Z-line was identified in 42% of PilICam videos and 17% of MiroCam, P = 0.0002. This information might be useful when perform- ing SBCE in patients with high risks for aspiration.
文摘AIM:To detect the prevalence of small bowel polyps by wireless capsule endoscopy(WCE)in patients with familial adenomatous polyposis(FAP).METHODS:We examined prospectively 14 patients with FAP to assess the location,size and number of small-intestinal polyps.Patients'age,sex,years of observation after surgery,type of surgery,duodenal polyps and colorectal cancer at surgery were analyzed.RESULTS:During WCE,polyps were detected in 9/14(64.3%)patients.Duodenal adenomatous polyps were found in nine(64.3%)patients,and jejunal and ileal polyps in seven(50%)and eight(57.1%),respectively.The Spigelman stage of duodenal polyposis was associated with the presence of jejunal and ileal polyps.Identification of the ampulla of Vater was not achieved with WCE.Importantly,the findings of WCE had no immediate impact on the further clinical management of FAP patients.No procedure-related complications were observed in the patients.CONCLUSION:WCE is a promising noninvasive new method for the detection of small-intestinal polyps.Further investigation is required to determine which phenotype of FAP is needed for surveillance with WCE.
基金financially supported by the National Key R&D Program of China (2019YFC1606603)。
文摘A novel lateral flow immunoassay biosensor,combining the receptor-ligand reaction and antigen-antibody reaction was developed for the detection of β-lactam antibiotics in milk. The receptor protein, the carboxy-terminal domain of β-lactam sensor-transducer mutant from Bacillus.licheniformis, served as the recognition element. Colloidal gold(CG)-labelled receptor antibody-receptor ternary complex was applied as a signal transducer probe. The presence ofβ-lactams was identified through a color change in the test zone, where the reaction between the complex of CG/antireceptor antibody/receptor and β-lactam antigen on the test line could be inhibited by β-lactam residues in the sample,thus leading to a reduction in color signal. Based on the indirect labeling of the receptor with an antibody as a linker, the ability to detect 33 β-lactams was below or near the corresponding maximum residue limit. The proposed lateral flow immunoassay biosensor appears to be an excellent field-based screening tool for the qualitative screening of β-lactams in milk.