Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the me...Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the mechanism.Methods:MTT,Transwell and three-dimentional culture were used to detect the proliferation,migration and tubule formation of SHG44.The expression of vascular endothelial growth factor-α(VEGF-α),erythropoietin-producing hepatocellular carcinoma-A2 (EphA2) and matrix metalloproteinases 2 (MMP2) was detected by RT-PCR and Western blotting analysis.Results:The OD 490 in hypoxia group was 0.60±0.06 and in control group was 0.46±0.05.The number of cell migration was 178.71±18.81 in hypoxia group and 85.86±17.92 in control group.The tubule formation was 56.80±12.21 in hypoxia group and 4.20±2.62 in control group.The proliferation,migration and tubule formation in hypoxia group were significantly higher than that in control group.The expression of VEGF-α,EphA2 and MMP2 was upregulated in hypoxia.When various concentrations of alphastatin (100,1 000,10 000 nmol/L) were added to hypoxia group,the numbers of cell migration were 142.57±12.12,92.71±17.68,30.00±7.72 and the tubule formation were 47.71±10.58,18.86±8.40,8.43±5.62.The cell migration and tubule formation were significantly suppressed by alphastatin in a dose-dependent manner.In alphastatin group,the phosphorylation of EphA2 protein (P=0.037,F=4.629) and activation of MMP2 protein (P=0.005,F=9.331) were significantly suppressed but there was no change in VEGF-α protein.Conclusion:Ⅱ-Ⅲ human glioma cell is able to form vasculogenic mimicry induced by hypoxia and alphastatin peptide can suppress the hypoxia-induced vasculogenic mimicry.VEGF-α induced EphA2 phospharilation and MMP2 activation maybe the key pathway to form vasculogenic mimicry.展开更多
The aerosol optical properties and chemical components of PM2.1(particulate matter with a diameter of 2.1μm or less)were investigated at Mount Gongga on the eastern slope of the Tibetan Plateau from April 2012 to Dec...The aerosol optical properties and chemical components of PM2.1(particulate matter with a diameter of 2.1μm or less)were investigated at Mount Gongga on the eastern slope of the Tibetan Plateau from April 2012 to December 2014.The annual mean aerosol optical depth(AOD)was 0.35±0.23,and the?ngstr?m exponent was 1.0±0.38.The AOD exhibited higher values in summer and winter,but lower values in spring and autumn.Dividing the observational periods into dry and wet seasons,the authors found that the concentrations of K^+,elemental carbon,secondary inorganic aerosols,and primary and secondary organic carbon in the dry(wet)season were 0.29(0.21),0.88(0.60),7.4(4.5),7.5(5.1),and 3.9(12)μg m?3,respectively.Combined with trajectory analysis,the authors found that higher concentrations of K^+,elemental carbon,and primary organic carbon indicated the effects of biomass burning from Southeast Asia during the dry season.However,the oxidation of volatile organic compounds was the main source of aerosols during the wet season,which originated from the Sichuan Basin.展开更多
Objective To identify the correlation between magnetic resonance manifestation and survival of patients with glioblastoma lnultiforme （GBM）, Methods The magnetic resonance imaging （MRI） images of 30 glioblastoma p...Objective To identify the correlation between magnetic resonance manifestation and survival of patients with glioblastoma lnultiforme （GBM）, Methods The magnetic resonance imaging （MRI） images of 30 glioblastoma patients were collected. Imaging features including degrees of contrasted area, edema surrounding the tumor; and intensity in T2-weighted imaging were selected to determine their correlation with patient survival. The relationship between imaging and survival time was studied using SPSS 19.0 software. Kaplan- Meier survival analysis and log-rank test were used to compare the survival curves. Results Patients with 〈5% contrasted enhancement area of tumor had longer overall survival （OS） than those with 〉5% contrasted enhancement area of tumor. Patients without edema surrounding the tumor had longer OS than those with edema. Patients with tumor of hyperintensity and/or isointensity in T2-weighted imaging had longer OS than those with hyperintensity and/or isointensity and hypointensity. Conclusions Some MR imaging features including degrees of contrasted area, edema surrounding the tumor, and intensity in T2- weighted imaging are correlated with the survival of patients with GBM. These features can serve as prognostic indicators for GBM patients.展开更多
基金Supported in Part by a Grant from the National Nature Science Foundation of China(No.30672126)
文摘Objective:To investigate the vasculogenic mimicry formation induced by hypoxia in Ⅱ-Ⅲ human glioma cell and the effect of alphastatin peptide suppressing the hypoxia-induced vasculogenic mimicry formation and the mechanism.Methods:MTT,Transwell and three-dimentional culture were used to detect the proliferation,migration and tubule formation of SHG44.The expression of vascular endothelial growth factor-α(VEGF-α),erythropoietin-producing hepatocellular carcinoma-A2 (EphA2) and matrix metalloproteinases 2 (MMP2) was detected by RT-PCR and Western blotting analysis.Results:The OD 490 in hypoxia group was 0.60±0.06 and in control group was 0.46±0.05.The number of cell migration was 178.71±18.81 in hypoxia group and 85.86±17.92 in control group.The tubule formation was 56.80±12.21 in hypoxia group and 4.20±2.62 in control group.The proliferation,migration and tubule formation in hypoxia group were significantly higher than that in control group.The expression of VEGF-α,EphA2 and MMP2 was upregulated in hypoxia.When various concentrations of alphastatin (100,1 000,10 000 nmol/L) were added to hypoxia group,the numbers of cell migration were 142.57±12.12,92.71±17.68,30.00±7.72 and the tubule formation were 47.71±10.58,18.86±8.40,8.43±5.62.The cell migration and tubule formation were significantly suppressed by alphastatin in a dose-dependent manner.In alphastatin group,the phosphorylation of EphA2 protein (P=0.037,F=4.629) and activation of MMP2 protein (P=0.005,F=9.331) were significantly suppressed but there was no change in VEGF-α protein.Conclusion:Ⅱ-Ⅲ human glioma cell is able to form vasculogenic mimicry induced by hypoxia and alphastatin peptide can suppress the hypoxia-induced vasculogenic mimicry.VEGF-α induced EphA2 phospharilation and MMP2 activation maybe the key pathway to form vasculogenic mimicry.
基金supported by the National Basic Research Program of China[grant numbers 2016YFC0202001 and 973Program 2014CB441200]the National Natural Science Foundation of China[grant numbers 41375036 and41305076]
文摘The aerosol optical properties and chemical components of PM2.1(particulate matter with a diameter of 2.1μm or less)were investigated at Mount Gongga on the eastern slope of the Tibetan Plateau from April 2012 to December 2014.The annual mean aerosol optical depth(AOD)was 0.35±0.23,and the?ngstr?m exponent was 1.0±0.38.The AOD exhibited higher values in summer and winter,but lower values in spring and autumn.Dividing the observational periods into dry and wet seasons,the authors found that the concentrations of K^+,elemental carbon,secondary inorganic aerosols,and primary and secondary organic carbon in the dry(wet)season were 0.29(0.21),0.88(0.60),7.4(4.5),7.5(5.1),and 3.9(12)μg m?3,respectively.Combined with trajectory analysis,the authors found that higher concentrations of K^+,elemental carbon,and primary organic carbon indicated the effects of biomass burning from Southeast Asia during the dry season.However,the oxidation of volatile organic compounds was the main source of aerosols during the wet season,which originated from the Sichuan Basin.
基金supported by a grant from the Chinese National Key Project of Science(No.30772238)
文摘Objective To identify the correlation between magnetic resonance manifestation and survival of patients with glioblastoma lnultiforme （GBM）, Methods The magnetic resonance imaging （MRI） images of 30 glioblastoma patients were collected. Imaging features including degrees of contrasted area, edema surrounding the tumor; and intensity in T2-weighted imaging were selected to determine their correlation with patient survival. The relationship between imaging and survival time was studied using SPSS 19.0 software. Kaplan- Meier survival analysis and log-rank test were used to compare the survival curves. Results Patients with 〈5% contrasted enhancement area of tumor had longer overall survival （OS） than those with 〉5% contrasted enhancement area of tumor. Patients without edema surrounding the tumor had longer OS than those with edema. Patients with tumor of hyperintensity and/or isointensity in T2-weighted imaging had longer OS than those with hyperintensity and/or isointensity and hypointensity. Conclusions Some MR imaging features including degrees of contrasted area, edema surrounding the tumor, and intensity in T2- weighted imaging are correlated with the survival of patients with GBM. These features can serve as prognostic indicators for GBM patients.
