蕨菜黄酮提取物免疫调节作用的实验研究

目的:研究蕨菜黄酮提取物对小鼠免疫功能的影响。方法:实验设正常对照组、蕨菜黄酮低剂量组、中剂量组、高剂量组。小鼠灌胃40天后,测定小鼠的脏器体重比值,观察迟发型变态反应(DTH)、单核-巨噬细胞碳廓清和血清溶血素等指标。结果:蕨菜黄酮提取物中剂量能显著提高小鼠足跖肿胀度,显著增强巨噬细胞的吞噬功能,显著提高血清溶血素抗体生成。结论:蕨菜黄酮对免疫功能有一定的增强作用。

海帕刺桐碱药理作用的研究

目的:探讨海帕刺桐碱的药理作用。方法:采用小鼠耳廓肿胀法、免疫器官重量和溶血素生成方法,四氯化碳(CCl4)和异硫氰酸萘酯制备肝损伤模型;观察海帕刺桐碱抗炎、免疫增强和抗肝损伤作用。结果:海帕刺桐碱大、小剂量组均明显减少小鼠耳廓重量;增加小鼠胸腺、脾脏重量,提高小鼠血清溶血素水平;明显降低CCl4和异硫氰酸萘酯所致肝损伤小鼠血清中转氨酶活性及胆红素含量。结论:海帕刺桐碱具有抗炎、免疫增强和抗肝损伤作用。

蜂胶增强蜂皇浆生物活性作用的实验研究

目的:探讨蜂胶时蜂皇浆生物活性的增强作用。方法:观察皇浆蜂胶粉对中老年大鼠免疫功能、性腺器官及血常规的影响,并以皇浆和蜂胶作对照组。结果:3种药物均可明显提高大鼠T琳转功能、NK细胞活性、MΦ吞噬功能和胸腺重量,以皇浆蜂胶粉作用最明显。另外,皇浆蜂胶粉和皇浆粉可明显增加中老年大鼠卵巢重量。对血常规均无明显影响。结论:蜂胶可明显增强蜂皇浆的增强免疫功能和延表作用。

蜂花粉对蛋鸡免疫器官组织结构的影响

目的:探讨蜂花粉对蛋鸡免疫器官发育的影响。方法:取200羽50日龄健康罗曼褐壳蛋鸡,随机分为2组,对照组饲喂基础日粮,实验组在基础日粮中添加1.5%的蜂花粉,分别于90、150、210、380天每组随机取鸡6羽,颈静脉放血致死,解剖取胸腺、腔上囊和脾,称重并取材,制作石蜡切片,显微镜下观察。结果:90天实验组蛋鸡的胸腺和腔上囊平均质量和器官指数极显著高于对照组(P<0.01),脾脏平均质量显著高于对照组(P<0.05);150天脾脏和腔上囊平均质量和器官指数显著或极显著高于对照组(P<0.05或P<0.01);210天腔上囊的平均质量和器官指数极显著高于对照组(P<0.01);380天除脾脏器官指数差异显著外,其他差异不显著。实验组的腔上囊小结皮质增厚,退化时间延迟;胸腺小叶皮质和脾的动脉周围淋巴鞘增厚,380天胸腺皮质明显厚于对照组。结论:蜂花粉可明显促进蛋鸡免疫器官早期发育,延缓中枢免疫器官的退化。

益寿灵对免疫功能影响的实验研究

目的:观察益寿灵(YSL)对免疫功能的影响。方法:分别用氢化可的松、环磷酰胺和醋酸氢化泼尼松造成小鼠免疫功能低下模型,观察YSL对免疫器官重量、单核巨噬细胞吞噬功能、淋巴细胞ANAE阳性率和血清溶血素水平的影响。结果:YSL具有对抗免疫功能低下模型小鼠免疫器官指数、单核巨噬细胞吞噬能力、淋巴细胞ANAE阳性率和溶血素水平下降的作用。结论:YSL具有增强机体非特异免疫、细胞免疫、体液免疫的作用。

Design,synthesis,and biological evaluation of novel benzimidazole derivatives and their interaction with calf thymus DNA and synergistic effects with clinical drugs

A series of new benzimidazole derivatives was synthesized and characterized by IR,1H NMR,13C NMR,MS,and HRMS spectra.All the new compounds were screened for their antimicrobial activities in vitro by a twofold serial dilution technique.The bioactive evaluation showed that 3,5-bis(trifluoromethyl)phenyl benzimidazoles were comparably or even more strongly antibacterial and antifungal than the reference drugs Chloromycin,Norfloxacin,and Fluconazole.The combination of2,4-difluorobenzyl benzimidazole derivative 5l and its hydrochloride 7 respectively with the antibacterials Chloromycin,Norfloxacin,and the antifungal Fluconazole was more sensitive to methicillin-resistant MRSA and Fluconazole-insensitive A.flavus.In addition,the interaction of compound 5l with calf thymus DNA demonstrated that this compound could effectively intercalate into DNA to form a compound 5l-DNA complex that might block DNA replication and thereby exert good antimicrobial activity.