A novel Ni-based metallic glass, i.e., Ni68.6W17.9B13.5(mole fraction, %), was prepared using melt spinning method. The results showed that the element B has much effect on the glass forming ability of the Ni-W-B me...A novel Ni-based metallic glass, i.e., Ni68.6W17.9B13.5(mole fraction, %), was prepared using melt spinning method. The results showed that the element B has much effect on the glass forming ability of the Ni-W-B metallic glass. Ni-W-B metallic glass could not be fabricated with lower content of B, whereas a higher content of 13.5% B could easily lead to the fully amorphous structure. The glass transition temperature and the onset temperature of crystallization are as high as 768 K and 781.5 K, respectively, and the crystallization activation energy calculated by Ozawa model is(637±60) k J/mol, which showed strong thermal stability of Ni68.6W17.9B13.5 metallic glass. This novel Ni-based metallic glass also exhibited good mechanical properties with the tensile strength of about 2331 MPa. The results indicate that this metallic glass should have a promising application in high strength material.展开更多
By varying concentration of PEG1000 as a structure-directing agent,mesoporous alumina with excellent textural properties was synthesized.The prepared mesoporous alumina displays high thermal stability,as shown by its ...By varying concentration of PEG1000 as a structure-directing agent,mesoporous alumina with excellent textural properties was synthesized.The prepared mesoporous alumina displays high thermal stability,as shown by its textural properties at different calcination temperatures of 600-850 °C.Characterization by SEM and TEM revealed that the added PEG surfactant induced the formation of petal-like alumina.XRD results clarified that all samples were amorphous and their peaks were around the peaks of γ-alumina.N_2 adsorption-desorption analysis showed that the prepared mesoporous alumina,if with PEG1000 in hydrolysis of aluminum isopropoxide,had excellent textural properties with large specific surface area,high pore volume and suitable pore size.The petal-like structure existing in the alumina samples improved their textural parameters,and the role and influential mechanism of PEG1000 were analyzed.展开更多
Metagenomic approaches are recently used for searching novel open reading frames (ORFs) coding enzymes employed in pharmaceutical, rood industries, etc. In this study, a metagenornic library was constructed from Chu...Metagenomic approaches are recently used for searching novel open reading frames (ORFs) coding enzymes employed in pharmaceutical, rood industries, etc. In this study, a metagenornic library was constructed from Chumathang hotspring sediment DNA. The library consisted of approximately 9,000 clones and was screened for protease activity. A clone exhibiting protease activity was identified and named CHprol. Sequencing of CHprol revealed that the ORF encoded a functional protein of 363 amino acids belonging to peptidase S8-S53 superfamily. CHprol shared 41% sequence similarity with a reported protease (subtilase family) and 35 % structural similarity with the crystal structure of Pro-Tk sps. of Thermococcus kodarkaenasis. In silico modeling the 3D structure of CHprol showed that it has two beta sheets, 10 alpha helices and 11 strands. Catalytic triad prediction implied CHprol to be a serine protease. The optimum temperature and pH of the purified protease were found to be 80 ℃ and 11.0, respectively. The enzyme was active at 5 % concentration of hydrogen peroxide and retained 60 % of activity at 10 % concentration. The thermotolerant, alkalophilic and oxidant stable properties of the protease make it a potential can- didate for biotechnological applications.展开更多
In this paper, we show that sweet quaternary ammonium, pyridinium, and imidazolium cyclamates are cheap, thermally stable, surface and biologically active ionic liquids, with potential application as feeding deterrents.
Packed anode of microbial fuel cells(MFCs),commonly with a dense structure, suffers from the clogging,resulting in unsatisfied long-term stability of MFCs. Herein,we fabricate a biochar-based packed anode with a loose...Packed anode of microbial fuel cells(MFCs),commonly with a dense structure, suffers from the clogging,resulting in unsatisfied long-term stability of MFCs. Herein,we fabricate a biochar-based packed anode with a loose structure to enhance the long-term performance of MFCs equipped with packed anodes. The biochar, derived from cocklebur fruit, endows the packed anode with a loose structure but excellent conductivity. Once incorporated into MFCs, the biochar-based packed anode can yield comparable performance to benchmark materials. Particularly, the biochar-based MFCs present no obvious decrease of the power output during 150 days’ operation, which is attributed to the clogging-resistant effect induced by the loose structure of biochar-based anode. The cocklebur fruit-derived biochar can be a promising candidate for MFC anodes, and should facilitate both scaling-up and practical applications of MFCs.展开更多
基金Project(50774098)supported by the National Natural Science Foundation of ChinaProject(50721003)supported by Creative Research Group of the National Natural Science Foundation of ChinaProject(CX2013B054)supported by the Innovative Research Project for Postgraduates in Hunan Province,China
文摘A novel Ni-based metallic glass, i.e., Ni68.6W17.9B13.5(mole fraction, %), was prepared using melt spinning method. The results showed that the element B has much effect on the glass forming ability of the Ni-W-B metallic glass. Ni-W-B metallic glass could not be fabricated with lower content of B, whereas a higher content of 13.5% B could easily lead to the fully amorphous structure. The glass transition temperature and the onset temperature of crystallization are as high as 768 K and 781.5 K, respectively, and the crystallization activation energy calculated by Ozawa model is(637±60) k J/mol, which showed strong thermal stability of Ni68.6W17.9B13.5 metallic glass. This novel Ni-based metallic glass also exhibited good mechanical properties with the tensile strength of about 2331 MPa. The results indicate that this metallic glass should have a promising application in high strength material.
基金Supported by the National Basic Research Program of China(Y419012198)the National Natural Science Foundation of China(No.91534125)
文摘By varying concentration of PEG1000 as a structure-directing agent,mesoporous alumina with excellent textural properties was synthesized.The prepared mesoporous alumina displays high thermal stability,as shown by its textural properties at different calcination temperatures of 600-850 °C.Characterization by SEM and TEM revealed that the added PEG surfactant induced the formation of petal-like alumina.XRD results clarified that all samples were amorphous and their peaks were around the peaks of γ-alumina.N_2 adsorption-desorption analysis showed that the prepared mesoporous alumina,if with PEG1000 in hydrolysis of aluminum isopropoxide,had excellent textural properties with large specific surface area,high pore volume and suitable pore size.The petal-like structure existing in the alumina samples improved their textural parameters,and the role and influential mechanism of PEG1000 were analyzed.
基金supported by Council of Scientific and Industrial Research(CSIR)New Delhi,Government of India(37(1545)/12/EMR-II)entitled‘‘Exploring Microbial DiversityMining Novel Hydrolases from Brackish Water Lakes of Ladakh Region by Metagenomic Approach.’’
文摘Metagenomic approaches are recently used for searching novel open reading frames (ORFs) coding enzymes employed in pharmaceutical, rood industries, etc. In this study, a metagenornic library was constructed from Chumathang hotspring sediment DNA. The library consisted of approximately 9,000 clones and was screened for protease activity. A clone exhibiting protease activity was identified and named CHprol. Sequencing of CHprol revealed that the ORF encoded a functional protein of 363 amino acids belonging to peptidase S8-S53 superfamily. CHprol shared 41% sequence similarity with a reported protease (subtilase family) and 35 % structural similarity with the crystal structure of Pro-Tk sps. of Thermococcus kodarkaenasis. In silico modeling the 3D structure of CHprol showed that it has two beta sheets, 10 alpha helices and 11 strands. Catalytic triad prediction implied CHprol to be a serine protease. The optimum temperature and pH of the purified protease were found to be 80 ℃ and 11.0, respectively. The enzyme was active at 5 % concentration of hydrogen peroxide and retained 60 % of activity at 10 % concentration. The thermotolerant, alkalophilic and oxidant stable properties of the protease make it a potential can- didate for biotechnological applications.
基金the National Science Centre (Poland) for financial support (7548/B/H03/2011/40)
文摘In this paper, we show that sweet quaternary ammonium, pyridinium, and imidazolium cyclamates are cheap, thermally stable, surface and biologically active ionic liquids, with potential application as feeding deterrents.
基金supported by the National Key R&D Program of China (2017YFA0207201)the National Natural Science Foundation of China (21507059)+3 种基金the Natural Science Foundation of Jiangsu Province (BK20150948)Six Talent Peaks Project in Jiangsu Province (JNHB-038)the Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of EducationYoung Elite Scientists Sponsorship Program by CAST (2017QNRC001)
文摘Packed anode of microbial fuel cells(MFCs),commonly with a dense structure, suffers from the clogging,resulting in unsatisfied long-term stability of MFCs. Herein,we fabricate a biochar-based packed anode with a loose structure to enhance the long-term performance of MFCs equipped with packed anodes. The biochar, derived from cocklebur fruit, endows the packed anode with a loose structure but excellent conductivity. Once incorporated into MFCs, the biochar-based packed anode can yield comparable performance to benchmark materials. Particularly, the biochar-based MFCs present no obvious decrease of the power output during 150 days’ operation, which is attributed to the clogging-resistant effect induced by the loose structure of biochar-based anode. The cocklebur fruit-derived biochar can be a promising candidate for MFC anodes, and should facilitate both scaling-up and practical applications of MFCs.