The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicate...The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro- viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under -20 degreesC, although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn (Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.展开更多
[Objective] This study was to provide theoretical basis for getting sustainable development of rural energy in Tibet into reality.[Method] By reviewing the rural energy resources in Tibet,we analyzed the characteristi...[Objective] This study was to provide theoretical basis for getting sustainable development of rural energy in Tibet into reality.[Method] By reviewing the rural energy resources in Tibet,we analyzed the characteristics and potential of rural biomass utilization in Tibet,and further put forward the sustainable countermeasures on the firewood substitution in Tibet.[Results] Renewable energies including hydraulic,geothermal,solar and wind resources are abundant in Tibet,while there is just a few of fossil energy resources such as oil and coal,with uneven distribution and poor exploration conditions.Traditional consumption of biomass energy resource accounts for a large proportion of the total energy consumption in Tibetan rural districts,which causes potential damage to the fragile ecological environment on the Tibetan Plateau.The excessive use of biomass energy destroyed the vegetation and evoked the environment deterioration such as the intensification of the water and soil loss and the declining of the soil fertility.[Conclusion] It is essential for Tibet to change its rural energy consumption structure,implement the Tibetan firewood alternative energy strategy and try to make full use of renewable energy such as solar energy,wind energy,hydro-energy instead of native vegetation and animal's droppings in order to reduce the adverse impacts of the irrational energy consumption on the ecological environment in Tibet.展开更多
Yak ( Bos grunniens ) is classified as Bovine genus,they live in Qingzang Plateau with elevation more than 3500m and it's neighbor highland,where the weather is high chilly,short of oxygen and large difference o...Yak ( Bos grunniens ) is classified as Bovine genus,they live in Qingzang Plateau with elevation more than 3500m and it's neighbor highland,where the weather is high chilly,short of oxygen and large difference of temperature between day and night.But yak have adapted that circumstance after thousands of years' artificial and natural selection and has great anti adversity capacity.Yak is an important sustenance and means of production to Tibetan.China is a country with the largest number of yak,whose amount of livestock on hand accounts for 95% of the world and Tibet region takes the second place next to Qinghai in the number of yak.Tibetan yak has formed some groups for Tibetan's large area,complicated geological ecosystem conditions,geological isolation.The typical better groups are Yadong yak,Jiali yak and Sibu yak. Study about yak fell behind other animals due to the restriction of inconvenient transportation,difficult sampling and poor experimental technology.Study about the relationship between yak blood enzyme activities and milk production was little,only Jing (1992) studied correlation of milk production with serum amylase,esterase in Gansu Jiulong yak,Maiwa yak and no report was found in other groups.This study discussed six Jiali yak blood enzyme activities lactate dehydrogenase (LDH),alkaline phosphatases (AKP),acid phosphatase (ACP),catalase (CAT),amylase (Amy) and superoxide dismutase (SOD),and explored their relationship with milk production in Jiali yak,so as to supply with theoretical evidence by using blood enzyme activity as biochemical assisted selection marker in the breeding of Tibetan yak milk yield. This experiment investigated activity of six blood enzymes from 28 Tibetan yaks and explored its relationship to production performance.The results showed a extremely positive significant difference ( P <0.01) between activity of LDH and milk yield,CAT activity and length of belly hair respectively,while a positive significant difference ( P <0.05) was observed between AKP activity and body weight,Amy activity and body weight and a negative significant difference ( P <0.05) for SOD activity and body weight.Stepwise Regression Analysis showed that activity of LDH could be used for the prediction of milk yield,AKP,CAT,Amy for body weight,CAT,Amy for length of belly hair.So it is expected to use activity of LDH,AKP,CAT,Amy as biochemical genetic marker in the selection of milk yield,body weight and length of belly hair.展开更多
[Objective] This study was conducted to understand goat germplasm char- acteristics. [Method] With some 24-month-old males and females Tibet goats as experimental materials, a slaughter experiment was carried out to d...[Objective] This study was conducted to understand goat germplasm char- acteristics. [Method] With some 24-month-old males and females Tibet goats as experimental materials, a slaughter experiment was carried out to determine their slaughter performance, muscle quality and muscle nutritional components. [Result] The body weights of males and females before slaughter were (20.16±1.26) and (19.33±4.86) kg, respectively; the dressing percentages of males and females were (43.86±1.74)% and (44.08±5.46)%, respectively; the meat percentages were (32.43± 1.52)% and (32.72±3.97)%, respectively; and the meat production rates of carcass were (73.28±0.09)% and (73.16±0.85)%, respectively. There were less differences in carcass measurements, carcass segmentation, muscle quality, muscle fiber charac- teristics, muscle nutritional components, muscle mineral contents and heavy metal contents between males and females, while eye muscle area and hind leg meat weight of males were significantly higher than those of females. In muscles of males and females, 18 amino acids were detected totally, EAA/TAA values were (39.23±0.24)% and (38.70±0.15)%, respectively, and EAA/NEAA values were (64.73± 0.65)% and (63.17±0.41)%, respectively. [Conclusion] This study lays a foundation for overall understanding of germplasm characteristics of Tibetan goats.展开更多
This study aimed to study the germplasm characteristics of Tibetan goat distributed in Sichuan. Three male and three female goats of twelve-month-old Ti-betan goat were selected, and they were slaughtered for analysis...This study aimed to study the germplasm characteristics of Tibetan goat distributed in Sichuan. Three male and three female goats of twelve-month-old Ti-betan goat were selected, and they were slaughtered for analysis of slaughter per-formance, muscle quality and muscle nutrients. The results showed that the pre-slaughter body weights, dressing percentages, meat percentages and carcass meat production rates of male and female Tibetan goats were (14.33±1.53) and (12.50±2.18) kg, (42.11±5.26)% and (43.85±4.51)%, (31.34±3.36)% and (32.84±3.16)%, and (72.34±0.54)% and (72.30±0.78)%, respectively. There were smal differences in car-cass size, carcass segmentation, muscle fiber properties, muscle quality, muscle nu-tritional composition, muscle minerals contents and muscle heavy metals contents between male and female Tibetan goats. A total of 18 kinds of amino acids were detected in the muscle of male and female Tibetan goats. The EAA/TAA ratios and EAA/NEAA ratios of male and female Tibetan goats were (39.18&#177;0.38)%and (38.70±0.72)%, and (64.31±1.03)% and (63.21±1.92)%, respectively.展开更多
The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa...The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)], and the triploid clone B346 [(P.tomentosa×P. bolleana)×(P. alba×P.glandulosa)], under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment_PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment_PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.展开更多
To make digital watermarking accomplish several goals, a new method for simultaneously embedding multiple watermarks into the same audio signal is proposed. First, the original audio signal is segmented into frames of...To make digital watermarking accomplish several goals, a new method for simultaneously embedding multiple watermarks into the same audio signal is proposed. First, the original audio signal is segmented into frames of appointed lengths and all the element watermarks are encoded to achieve a mixed watermark. Then, the binary bits in the mixed watermark are embedded into the audio frames with the echo hiding technique. The watermark extraction can be performed without an original audio signal. Furthermore, in order to enhance the extraction accuracy and the robustness of the proposed algorithm against common signal manipulations, the autocorrelafion of the power cepstrum is utilized to estimate the echo delays in the watermarked audio frames to extract the mixed watermark and the corresponding decoding method is applied to achieve the element watermarks. Computer simulation results indicate that the proposed scheme has great robustness against common signal manipulations of Mp3 compressing, re-sampling, re-quantizing, low-pass filtering and white noise addition.展开更多
Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It...Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It has many good traits, such as large fruit size, early fruit-setting and high yield, excellent eating quality, and good environmental adaptability. However, there are some shortcomings with the fruit, including thick skin, excessive number of seeds, and relatively small proportion of edible flesh. Akebia is also a traditional Chinese medicine, which has the functions of relieving internal heat and diuresis, dredging venation and improving blood circulation, cleansing facial chloasma, detoxication and anti-cancer activities. Akebia can be used as fresh fruit, for processing, and for pharmaceutical purposes. We have been undertaking a series of research on the domestication and utilization of Akebia, including cultivar selection, techniques for cultivation, storage and processing, and related basic studies. A Chinese trade name “Hehuanguo” and an English trade name “Joyfruit” have been registered for Akebia to facilitate its commercial development.展开更多
Objective: To explore the mechanism of native Tibetan fetuses adaptation to hypoxia, we tried to find the different expression genes about mitochondrial function in the native Tibetan placents. Methods: In this stud...Objective: To explore the mechanism of native Tibetan fetuses adaptation to hypoxia, we tried to find the different expression genes about mitochondrial function in the native Tibetan placents. Methods: In this study, the placents of native Tibetan and the high-altitude Hart (ha-Hart) were collected. After the total RNA extraction, the finally synthesized cDNAs were hybridized to mitochondrial array to find the altered expression genes between them. Then, the cytochrome c oxidase 17 (Coxl7), dynactin 2 (DCTN2, also known as p50), and vascular endothelial growth factor receptor (VEGFR, also known as KDR) were chosen from the altered expression genes to further verify the array results using the SYBR Green real-time PCR. Because the altered expression genes (such as Cybb and Cox 17) in the array results related to the activities of COXI and COXIV, the placental mitochondria activities of COXI and COXIV were measured to find their changes in the hypoxia. Results: By a standard of≥1.5 or ≤0.67, there were 24 different expressed genes between the native Tibetan and the ha-Han placents, including 3 up-regulated genes and 21 down-regulated genes. These genes were related to energy metabolism, signal transduction, cell proliferation, electron transport, cell adhesion, nucleotide-excision repair. The array results of Cox17, DCTN2 and KDR were further verified by the real-time RT-PCR. Through the mitochondria respiration measurements, the activity of COXI in the native Tibetan placents were higher than that of ha-Han, there was no difference in COXIV activity between them. Conclusion: The altered mitochondrial related genes in the native Tibetan placents may have a role in the high altitude adaptation for fetuses through changing the activity of mitochondrial COX.展开更多
To protect trains against strong cross-wind along Qinghai-Tibet railway, a strong wind speed monitoring and warning system was developed. And to obtain high-precision wind speed short-term forecasting values for the s...To protect trains against strong cross-wind along Qinghai-Tibet railway, a strong wind speed monitoring and warning system was developed. And to obtain high-precision wind speed short-term forecasting values for the system to make more accurate scheduling decision, two optimization algorithms were proposed. Using them to make calculative examples for actual wind speed time series from the 18th meteorological station, the results show that: the optimization algorithm based on wavelet analysis method and improved time series analysis method can attain high-precision multi-step forecasting values, the mean relative errors of one-step, three-step, five-step and ten-step forecasting are only 0.30%, 0.75%, 1.15% and 1.65%, respectively. The optimization algorithm based on wavelet analysis method and Kalman time series analysis method can obtain high-precision one-step forecasting values, the mean relative error of one-step forecasting is reduced by 61.67% to 0.115%. The two optimization algorithms both maintain the modeling simple character, and can attain prediction explicit equations after modeling calculation.展开更多
In order to better design, fabricate and control pear handling machine, we should take into account mechanical and rheological properties of pear fruits as related to handling process. The changes in rheological prope...In order to better design, fabricate and control pear handling machine, we should take into account mechanical and rheological properties of pear fruits as related to handling process. The changes in rheological properties of pears stored at 5, 15, 25 ℃ and variable (fluctuating) temperature for 12 days were evaluated in terms of elasticity and viscosity parameters using creep tests. The elasticity and viscosity parameters in creep tests in general decreased with increase in storage time both under constant and variable storage conditions. For the variable storage condition, a bulk mean temperature calculated to account for a series combination of storage time and temperature to which the pears subjected. The changes in rheological properties due to variable storage temperature were described as a function of storage time. The result indicated that except the viscosity parameter of the Maxwell component of the four-element model, it was possible to describe the changes in rheological properties as a function of storage time, which are better physical parameters to estimate the quality of pears.展开更多
In order to investigate the resistance of female silkworm moths to cold storage and their copulation and oviposition after cold storage, copulation and ovipo- sition experiments of Liangguang 2 silkworms and Guican 1 ...In order to investigate the resistance of female silkworm moths to cold storage and their copulation and oviposition after cold storage, copulation and ovipo- sition experiments of Liangguang 2 silkworms and Guican 1 female silkworm moths were conduced under cold storage at 5 ~C for different durations. The results indi- cated that, after cold storage at 5 ~C with relative humidity of 75%, the resistance of female silkworm moths to cold storage varied with different varieties; to be specific, 9-Fu and 7.Xiang, parent silkworm varieties of Liangguang 2 with ancestry of mul- tivoltinism, were relatively resistant to cold storage, and their copulation capability was still at a high level after cold storage for 72 h. Oviposition capability also varied with different varieties and durations; to be specific, under 5 ~C conditions, between two parent silkworm varieties of Liangguang 2, female silkworm moths of 9-Fu achieved the best results within cold storage duration of 24 h, female silkworm moths of 7.Xiang achieved the best results within cold storage duration of 72 h; be- tween two parent silkworm varieties of Guican 1, female silkworm moths of Xinhang achieved the best results without cold storage, female silkworm moths of 826 achieved the best results within cold storage duration of 48 h.展开更多
[ Objective] The aim was to study the antioxidative index of the compound beverage of winter jujube and peanut meal, discuss the im-pact of the storage conditions on its antioxidative activity and provide theoretical ...[ Objective] The aim was to study the antioxidative index of the compound beverage of winter jujube and peanut meal, discuss the im-pact of the storage conditions on its antioxidative activity and provide theoretical basis for the utilization of winter jujube and peanut meal resources. [Method] A kind of compound beverage was prepared with winter jujube and peanut meal, and its antioxidative activity was detected and its best storage conditions were determined. [ Result] The capacity of the compound beverage stored for 20 d to scavenge · OH, DPPH · and nitroso was the highest at freeze conditions, then followed by the refrigeration conditions, room temperature storage and sunlight. The total reducing capacity of the products was the highest under freezing conditions while the lowest under sunlight when some active components were destroyed. [ Conclusion] It is the best to store the compound beverage of winter jujube and peanut meal at freeze conditions and better at the refrigeration conditions. The storage of compound beverage should avoid direct sunlight. Key words Winter jujube; Peanut meal; Compound beverage; Antioxidation; China展开更多
Chemical application after harvest is an important method to preserve strawberry fruit quality and extend shelf life. The strawberry fruits harvested at red stage were treated with three different sources of calcium, ...Chemical application after harvest is an important method to preserve strawberry fruit quality and extend shelf life. The strawberry fruits harvested at red stage were treated with three different sources of calcium, i.e., calcium nitrate, calcium gluconate and calcium chloride at calcium concentration of 0% (distilled water), 0.5%, 1.0% and 1.5%. The strawberry fruits were dipped for 30 s. The calcium sources, calcium concentration and the interaction of calcium sources and calcium concentration significantly affected the storage performance of strawberry fruits. All the three sources and concentrations were effective in decreasing the storage associated changes, i.e., weight loss, decline in marketable fruits, loss of organoleptic quality, increase in total soluble solids (TSS), decline in reducing sugars, total sugars, acidity and increase in sugar/acid ratio in strawberry fruit, thus extending the shelf life. Calcium gluconate at 1.5% calcium concentration was more effective. Treatment of strawberry fruit with 1.5% calcium from calcium gluconate source resulted in the highest number of marketable fruit (100%), extended shelf life (10 d), and total soluble solid (7.80%) with the minimum weight loss (5.45%). Calcium dips result in higher calcium concentration in strawberry fruits, which delays ripening in fruits by maintaining the structure and function of cell walls and membranes. It can be concluded that calcium gluconate at 1.5% concentration is an effective calcium treatment to retain the quality and extend the shelf life of strawberry fruit.展开更多
The purpose of this paper is to evaluate the possibility of implementation of alternative renewable energy micro-generation installations in dairy farm milk collection posts, from the technical, economical and environ...The purpose of this paper is to evaluate the possibility of implementation of alternative renewable energy micro-generation installations in dairy farm milk collection posts, from the technical, economical and environmental perspectives. Work was performed through dairy farm milk collection post energy audits. Weather data was obtained from CLIMAAT and MacSIMAR projects and introduced in a renewable energy solutions calculator (HOMER). Moreover, a system design from the available portfolio of micro-generation devices was defined and a cost benefit analysis of the selected alternatives was performed. From the analyzed solutions, the set of PV (20 kW) and Wind (3 kW) with no batteries revealed to be the best one. The sensitivity analysis indicated that wind micro-turbines should be installed except if the solar radiation is maximum and that in no case the use of batteries is an advantage. For systems requiring a certain degree of energy (in this case with an average of 350 kW/day), the implementation of micro generation systems, like the ones evaluated, will never be economically feasible in terms of Total Economic Value, even with generous feed-in tariff schemes. It is preferable that grid operators make investments in renewable energy production.展开更多
Knowledge of the spatial distribution of permafrost and the effects of climate on ground temperature are important for land use and infrastructure development on the Qinghai-Tibet Plateau(QTP). Different permafrost mo...Knowledge of the spatial distribution of permafrost and the effects of climate on ground temperature are important for land use and infrastructure development on the Qinghai-Tibet Plateau(QTP). Different permafrost models have been developed to simulate the ground temperature and active layer thickness(ALT). In this study, Temperature at Top of Permafrost(TTOP) model, Kudryavtsev model and modified Stefan solution were evaluated against detailed field measurements at four distinct field sites in the Wudaoliang Basin to better understand the applicability of permafrost models. Field data from 2012 to 2014 showed that there were notable differences in observed ground temperatures and ALTs within and among the sites. The TTOP model is relatively simple, however, when driven by averaged input values, it produced more accurate permafrost surface temperature(Tps) than the Kudryavtsev model. The modified Stefan solution resulted in a satisfactory accuracy of 90%, which was better than the Kudryavtsev model for estimating ALTs. The modified Stefan solution had the potential of being applied to climate-change studies in the future. Furthermore, additional field investigations over longer periods focusing on hydrology, which has significant influence on permafrost thaw, are necessary. These efforts should employ advanced measurement techniques to obtain adequate and extensive local parameters that will help improve model accuracy.展开更多
With the popularity of smartphones and the rapid development of mobile internet, smartphone becomes an important tool that store sensitive data of owner. Encryption naturally becomes a necessary means of protection. I...With the popularity of smartphones and the rapid development of mobile internet, smartphone becomes an important tool that store sensitive data of owner. Encryption naturally becomes a necessary means of protection. In certain situations, this is inadequate, as user may be coerced to hand over decryption keys or passwords of sensitive APP(Ali Pay) on mobile device. Therefore, only encryption cannot protect sensitive APP and privacy data stored on user's smartphone. To address these obstacles, we design a protection system called Mobi Gemini. It enables automatic uninstalling service that can immediately uninstall multiple APP at same time, and also enabling plausibly deniable encryption(PDE) on mobile devices by hiding encrypted volume within random data in free space of cache partition. We improve the key store way of previous PDE schemes on mobile device. The evaluation results show that the scheme introduces a few overhead compared with original android system enabling full disk encryption.展开更多
文摘The effects of ultradry storage on the activity of ATPase and fluidity of plasma membrane in Chinese cabbage (Brassica pekinensis (Lour.) Rupr.) and elm ( Ulmus pumila L.) seeds were investigated. The results indicated that no significant differences in the activity of ATPase and the micro- viscosity of plasma membrane of ultradried (UD) seeds could be found as compared to the control seeds stored under -20 degreesC, although there was a little adverse effect on the seeds with extreme dehydration. The results were consistent with higher vigor level of UD seeds. This implied that ultradry seed storage could protect the integrity of the membrane, maintain its physiological function and improve the storability of seeds. The relationship between sugar and desiccation tolerance of UD seeds was analysed using the HPLC. The results showed that the ratio of reducing to nonreducing sugar was lower in UD seeds than that in control. The content of sucrose and stachyose in elm and Chinese cabbage UD seeds was related to their desiccation tolerance, while no stachyose was detected in corn (Zea mays L.) seeds. This could be one of the reasons for its sensitivity to desiccation.
基金Supported by Chinese Academy of Sciences-Knowledge Innovation Program Key Direction Project(KZCX2-YW-310)National Basic Research Program of China(2010CB951702)~~
文摘[Objective] This study was to provide theoretical basis for getting sustainable development of rural energy in Tibet into reality.[Method] By reviewing the rural energy resources in Tibet,we analyzed the characteristics and potential of rural biomass utilization in Tibet,and further put forward the sustainable countermeasures on the firewood substitution in Tibet.[Results] Renewable energies including hydraulic,geothermal,solar and wind resources are abundant in Tibet,while there is just a few of fossil energy resources such as oil and coal,with uneven distribution and poor exploration conditions.Traditional consumption of biomass energy resource accounts for a large proportion of the total energy consumption in Tibetan rural districts,which causes potential damage to the fragile ecological environment on the Tibetan Plateau.The excessive use of biomass energy destroyed the vegetation and evoked the environment deterioration such as the intensification of the water and soil loss and the declining of the soil fertility.[Conclusion] It is essential for Tibet to change its rural energy consumption structure,implement the Tibetan firewood alternative energy strategy and try to make full use of renewable energy such as solar energy,wind energy,hydro-energy instead of native vegetation and animal's droppings in order to reduce the adverse impacts of the irrational energy consumption on the ecological environment in Tibet.
文摘Yak ( Bos grunniens ) is classified as Bovine genus,they live in Qingzang Plateau with elevation more than 3500m and it's neighbor highland,where the weather is high chilly,short of oxygen and large difference of temperature between day and night.But yak have adapted that circumstance after thousands of years' artificial and natural selection and has great anti adversity capacity.Yak is an important sustenance and means of production to Tibetan.China is a country with the largest number of yak,whose amount of livestock on hand accounts for 95% of the world and Tibet region takes the second place next to Qinghai in the number of yak.Tibetan yak has formed some groups for Tibetan's large area,complicated geological ecosystem conditions,geological isolation.The typical better groups are Yadong yak,Jiali yak and Sibu yak. Study about yak fell behind other animals due to the restriction of inconvenient transportation,difficult sampling and poor experimental technology.Study about the relationship between yak blood enzyme activities and milk production was little,only Jing (1992) studied correlation of milk production with serum amylase,esterase in Gansu Jiulong yak,Maiwa yak and no report was found in other groups.This study discussed six Jiali yak blood enzyme activities lactate dehydrogenase (LDH),alkaline phosphatases (AKP),acid phosphatase (ACP),catalase (CAT),amylase (Amy) and superoxide dismutase (SOD),and explored their relationship with milk production in Jiali yak,so as to supply with theoretical evidence by using blood enzyme activity as biochemical assisted selection marker in the breeding of Tibetan yak milk yield. This experiment investigated activity of six blood enzymes from 28 Tibetan yaks and explored its relationship to production performance.The results showed a extremely positive significant difference ( P <0.01) between activity of LDH and milk yield,CAT activity and length of belly hair respectively,while a positive significant difference ( P <0.05) was observed between AKP activity and body weight,Amy activity and body weight and a negative significant difference ( P <0.05) for SOD activity and body weight.Stepwise Regression Analysis showed that activity of LDH could be used for the prediction of milk yield,AKP,CAT,Amy for body weight,CAT,Amy for length of belly hair.So it is expected to use activity of LDH,AKP,CAT,Amy as biochemical genetic marker in the selection of milk yield,body weight and length of belly hair.
基金Supported by Special Fund for Animal Husbandry Science and Technology in Sichuan Province~~
文摘[Objective] This study was conducted to understand goat germplasm char- acteristics. [Method] With some 24-month-old males and females Tibet goats as experimental materials, a slaughter experiment was carried out to determine their slaughter performance, muscle quality and muscle nutritional components. [Result] The body weights of males and females before slaughter were (20.16±1.26) and (19.33±4.86) kg, respectively; the dressing percentages of males and females were (43.86±1.74)% and (44.08±5.46)%, respectively; the meat percentages were (32.43± 1.52)% and (32.72±3.97)%, respectively; and the meat production rates of carcass were (73.28±0.09)% and (73.16±0.85)%, respectively. There were less differences in carcass measurements, carcass segmentation, muscle quality, muscle fiber charac- teristics, muscle nutritional components, muscle mineral contents and heavy metal contents between males and females, while eye muscle area and hind leg meat weight of males were significantly higher than those of females. In muscles of males and females, 18 amino acids were detected totally, EAA/TAA values were (39.23±0.24)% and (38.70±0.15)%, respectively, and EAA/NEAA values were (64.73± 0.65)% and (63.17±0.41)%, respectively. [Conclusion] This study lays a foundation for overall understanding of germplasm characteristics of Tibetan goats.
文摘This study aimed to study the germplasm characteristics of Tibetan goat distributed in Sichuan. Three male and three female goats of twelve-month-old Ti-betan goat were selected, and they were slaughtered for analysis of slaughter per-formance, muscle quality and muscle nutrients. The results showed that the pre-slaughter body weights, dressing percentages, meat percentages and carcass meat production rates of male and female Tibetan goats were (14.33±1.53) and (12.50±2.18) kg, (42.11±5.26)% and (43.85±4.51)%, (31.34±3.36)% and (32.84±3.16)%, and (72.34±0.54)% and (72.30±0.78)%, respectively. There were smal differences in car-cass size, carcass segmentation, muscle fiber properties, muscle quality, muscle nu-tritional composition, muscle minerals contents and muscle heavy metals contents between male and female Tibetan goats. A total of 18 kinds of amino acids were detected in the muscle of male and female Tibetan goats. The EAA/TAA ratios and EAA/NEAA ratios of male and female Tibetan goats were (39.18&#177;0.38)%and (38.70±0.72)%, and (64.31±1.03)% and (63.21±1.92)%, respectively.
文摘The oxygen evolution, thermal dissipation, and photochemical energy storage of three hybrid poplar clones, namely the triploid clone B342, the diploid clone B11 [(Populus alba×P. glandulosa)×(P.tomentosa×P.bolleana)], and the triploid clone B346 [(P.tomentosa×P. bolleana)×(P. alba×P.glandulosa)], under light stress were studied using photoacoustics. The oxygen evolution signal and photochemical energy storage varied negatively with the pretreatment_PFD (photon flux density), whereas the thermal signal varied positively with the pretreatment_PFD. Photochemical energy storage was reallocated to PSⅡ more than to PSⅠ, while the photochemical energy storage in PSⅠ was more stable than that in PSⅡ when subjected to light stress. The inhibitors streptomycin (SM), dithiothreitol (DTT) and sodium fluoride (NaF) could all affect the oxygen evolution signal. Clones B11 and B342 were more resistant to light stress than clone B346.
文摘To make digital watermarking accomplish several goals, a new method for simultaneously embedding multiple watermarks into the same audio signal is proposed. First, the original audio signal is segmented into frames of appointed lengths and all the element watermarks are encoded to achieve a mixed watermark. Then, the binary bits in the mixed watermark are embedded into the audio frames with the echo hiding technique. The watermark extraction can be performed without an original audio signal. Furthermore, in order to enhance the extraction accuracy and the robustness of the proposed algorithm against common signal manipulations, the autocorrelafion of the power cepstrum is utilized to estimate the echo delays in the watermarked audio frames to extract the mixed watermark and the corresponding decoding method is applied to achieve the element watermarks. Computer simulation results indicate that the proposed scheme has great robustness against common signal manipulations of Mp3 compressing, re-sampling, re-quantizing, low-pass filtering and white noise addition.
文摘Akebia is a wild fruit originating in the Yangtse river valley and the surrounding areas. It includes mainly three species (subspecies): Akebia trifoliata, Akebia trifoliata subsp, australis, and Akebia quinata. It has many good traits, such as large fruit size, early fruit-setting and high yield, excellent eating quality, and good environmental adaptability. However, there are some shortcomings with the fruit, including thick skin, excessive number of seeds, and relatively small proportion of edible flesh. Akebia is also a traditional Chinese medicine, which has the functions of relieving internal heat and diuresis, dredging venation and improving blood circulation, cleansing facial chloasma, detoxication and anti-cancer activities. Akebia can be used as fresh fruit, for processing, and for pharmaceutical purposes. We have been undertaking a series of research on the domestication and utilization of Akebia, including cultivar selection, techniques for cultivation, storage and processing, and related basic studies. A Chinese trade name “Hehuanguo” and an English trade name “Joyfruit” have been registered for Akebia to facilitate its commercial development.
基金Supported by the National Basic Research Program of China (973 Program, 2006CB504101)the National Natural Science Foundation of China (30393131)
文摘Objective: To explore the mechanism of native Tibetan fetuses adaptation to hypoxia, we tried to find the different expression genes about mitochondrial function in the native Tibetan placents. Methods: In this study, the placents of native Tibetan and the high-altitude Hart (ha-Hart) were collected. After the total RNA extraction, the finally synthesized cDNAs were hybridized to mitochondrial array to find the altered expression genes between them. Then, the cytochrome c oxidase 17 (Coxl7), dynactin 2 (DCTN2, also known as p50), and vascular endothelial growth factor receptor (VEGFR, also known as KDR) were chosen from the altered expression genes to further verify the array results using the SYBR Green real-time PCR. Because the altered expression genes (such as Cybb and Cox 17) in the array results related to the activities of COXI and COXIV, the placental mitochondria activities of COXI and COXIV were measured to find their changes in the hypoxia. Results: By a standard of≥1.5 or ≤0.67, there were 24 different expressed genes between the native Tibetan and the ha-Han placents, including 3 up-regulated genes and 21 down-regulated genes. These genes were related to energy metabolism, signal transduction, cell proliferation, electron transport, cell adhesion, nucleotide-excision repair. The array results of Cox17, DCTN2 and KDR were further verified by the real-time RT-PCR. Through the mitochondria respiration measurements, the activity of COXI in the native Tibetan placents were higher than that of ha-Han, there was no difference in COXIV activity between them. Conclusion: The altered mitochondrial related genes in the native Tibetan placents may have a role in the high altitude adaptation for fetuses through changing the activity of mitochondrial COX.
基金Project(2006BAC07B03) supported by the National Key Technology R & D Program of ChinaProject(2006G040-A) supported by the Foundation of the Science and Technology Section of Ministry of RailwayProject(2008yb044) supported by the Foundation of Excellent Doctoral Dissertation of Central South University
文摘To protect trains against strong cross-wind along Qinghai-Tibet railway, a strong wind speed monitoring and warning system was developed. And to obtain high-precision wind speed short-term forecasting values for the system to make more accurate scheduling decision, two optimization algorithms were proposed. Using them to make calculative examples for actual wind speed time series from the 18th meteorological station, the results show that: the optimization algorithm based on wavelet analysis method and improved time series analysis method can attain high-precision multi-step forecasting values, the mean relative errors of one-step, three-step, five-step and ten-step forecasting are only 0.30%, 0.75%, 1.15% and 1.65%, respectively. The optimization algorithm based on wavelet analysis method and Kalman time series analysis method can obtain high-precision one-step forecasting values, the mean relative error of one-step forecasting is reduced by 61.67% to 0.115%. The two optimization algorithms both maintain the modeling simple character, and can attain prediction explicit equations after modeling calculation.
文摘In order to better design, fabricate and control pear handling machine, we should take into account mechanical and rheological properties of pear fruits as related to handling process. The changes in rheological properties of pears stored at 5, 15, 25 ℃ and variable (fluctuating) temperature for 12 days were evaluated in terms of elasticity and viscosity parameters using creep tests. The elasticity and viscosity parameters in creep tests in general decreased with increase in storage time both under constant and variable storage conditions. For the variable storage condition, a bulk mean temperature calculated to account for a series combination of storage time and temperature to which the pears subjected. The changes in rheological properties due to variable storage temperature were described as a function of storage time. The result indicated that except the viscosity parameter of the Maxwell component of the four-element model, it was possible to describe the changes in rheological properties as a function of storage time, which are better physical parameters to estimate the quality of pears.
基金Supported by Special Fund for Construction of Modern Agricultural Industry Technology System of the Ministry of Agriculture(CARS-22-SYZ26)Innovation Ability Construction Project of Science and Technology Department of Guizhou Province(QKHYSCX[2012]4003)Fund of Guizhou Academy of Agricultural Sciences[QNKH(Fund)2011017]~~
文摘In order to investigate the resistance of female silkworm moths to cold storage and their copulation and oviposition after cold storage, copulation and ovipo- sition experiments of Liangguang 2 silkworms and Guican 1 female silkworm moths were conduced under cold storage at 5 ~C for different durations. The results indi- cated that, after cold storage at 5 ~C with relative humidity of 75%, the resistance of female silkworm moths to cold storage varied with different varieties; to be specific, 9-Fu and 7.Xiang, parent silkworm varieties of Liangguang 2 with ancestry of mul- tivoltinism, were relatively resistant to cold storage, and their copulation capability was still at a high level after cold storage for 72 h. Oviposition capability also varied with different varieties and durations; to be specific, under 5 ~C conditions, between two parent silkworm varieties of Liangguang 2, female silkworm moths of 9-Fu achieved the best results within cold storage duration of 24 h, female silkworm moths of 7.Xiang achieved the best results within cold storage duration of 72 h; be- tween two parent silkworm varieties of Guican 1, female silkworm moths of Xinhang achieved the best results without cold storage, female silkworm moths of 826 achieved the best results within cold storage duration of 48 h.
文摘[ Objective] The aim was to study the antioxidative index of the compound beverage of winter jujube and peanut meal, discuss the im-pact of the storage conditions on its antioxidative activity and provide theoretical basis for the utilization of winter jujube and peanut meal resources. [Method] A kind of compound beverage was prepared with winter jujube and peanut meal, and its antioxidative activity was detected and its best storage conditions were determined. [ Result] The capacity of the compound beverage stored for 20 d to scavenge · OH, DPPH · and nitroso was the highest at freeze conditions, then followed by the refrigeration conditions, room temperature storage and sunlight. The total reducing capacity of the products was the highest under freezing conditions while the lowest under sunlight when some active components were destroyed. [ Conclusion] It is the best to store the compound beverage of winter jujube and peanut meal at freeze conditions and better at the refrigeration conditions. The storage of compound beverage should avoid direct sunlight. Key words Winter jujube; Peanut meal; Compound beverage; Antioxidation; China
文摘Chemical application after harvest is an important method to preserve strawberry fruit quality and extend shelf life. The strawberry fruits harvested at red stage were treated with three different sources of calcium, i.e., calcium nitrate, calcium gluconate and calcium chloride at calcium concentration of 0% (distilled water), 0.5%, 1.0% and 1.5%. The strawberry fruits were dipped for 30 s. The calcium sources, calcium concentration and the interaction of calcium sources and calcium concentration significantly affected the storage performance of strawberry fruits. All the three sources and concentrations were effective in decreasing the storage associated changes, i.e., weight loss, decline in marketable fruits, loss of organoleptic quality, increase in total soluble solids (TSS), decline in reducing sugars, total sugars, acidity and increase in sugar/acid ratio in strawberry fruit, thus extending the shelf life. Calcium gluconate at 1.5% calcium concentration was more effective. Treatment of strawberry fruit with 1.5% calcium from calcium gluconate source resulted in the highest number of marketable fruit (100%), extended shelf life (10 d), and total soluble solid (7.80%) with the minimum weight loss (5.45%). Calcium dips result in higher calcium concentration in strawberry fruits, which delays ripening in fruits by maintaining the structure and function of cell walls and membranes. It can be concluded that calcium gluconate at 1.5% concentration is an effective calcium treatment to retain the quality and extend the shelf life of strawberry fruit.
文摘The purpose of this paper is to evaluate the possibility of implementation of alternative renewable energy micro-generation installations in dairy farm milk collection posts, from the technical, economical and environmental perspectives. Work was performed through dairy farm milk collection post energy audits. Weather data was obtained from CLIMAAT and MacSIMAR projects and introduced in a renewable energy solutions calculator (HOMER). Moreover, a system design from the available portfolio of micro-generation devices was defined and a cost benefit analysis of the selected alternatives was performed. From the analyzed solutions, the set of PV (20 kW) and Wind (3 kW) with no batteries revealed to be the best one. The sensitivity analysis indicated that wind micro-turbines should be installed except if the solar radiation is maximum and that in no case the use of batteries is an advantage. For systems requiring a certain degree of energy (in this case with an average of 350 kW/day), the implementation of micro generation systems, like the ones evaluated, will never be economically feasible in terms of Total Economic Value, even with generous feed-in tariff schemes. It is preferable that grid operators make investments in renewable energy production.
基金funded by the State Key Development Program of Basic Research of China(973 Plan,Grant No.2012CB026101)the National Science and Technology Support Plan(Grant Nos.2014BAG05B01,2014BAG05B05)
文摘Knowledge of the spatial distribution of permafrost and the effects of climate on ground temperature are important for land use and infrastructure development on the Qinghai-Tibet Plateau(QTP). Different permafrost models have been developed to simulate the ground temperature and active layer thickness(ALT). In this study, Temperature at Top of Permafrost(TTOP) model, Kudryavtsev model and modified Stefan solution were evaluated against detailed field measurements at four distinct field sites in the Wudaoliang Basin to better understand the applicability of permafrost models. Field data from 2012 to 2014 showed that there were notable differences in observed ground temperatures and ALTs within and among the sites. The TTOP model is relatively simple, however, when driven by averaged input values, it produced more accurate permafrost surface temperature(Tps) than the Kudryavtsev model. The modified Stefan solution resulted in a satisfactory accuracy of 90%, which was better than the Kudryavtsev model for estimating ALTs. The modified Stefan solution had the potential of being applied to climate-change studies in the future. Furthermore, additional field investigations over longer periods focusing on hydrology, which has significant influence on permafrost thaw, are necessary. These efforts should employ advanced measurement techniques to obtain adequate and extensive local parameters that will help improve model accuracy.
基金supported in part by Natural Science Foundation of China under (Grant No. U1536112) National Key Technology Research and Development Program of China (Grant No. 2012BAH94F02)+1 种基金National High-tech R&D Program of China (863 Program) under Grant No. 2013AA102301 Project of New Generation Broad band Wireless Network under Grant No. 2014ZX03006003
文摘With the popularity of smartphones and the rapid development of mobile internet, smartphone becomes an important tool that store sensitive data of owner. Encryption naturally becomes a necessary means of protection. In certain situations, this is inadequate, as user may be coerced to hand over decryption keys or passwords of sensitive APP(Ali Pay) on mobile device. Therefore, only encryption cannot protect sensitive APP and privacy data stored on user's smartphone. To address these obstacles, we design a protection system called Mobi Gemini. It enables automatic uninstalling service that can immediately uninstall multiple APP at same time, and also enabling plausibly deniable encryption(PDE) on mobile devices by hiding encrypted volume within random data in free space of cache partition. We improve the key store way of previous PDE schemes on mobile device. The evaluation results show that the scheme introduces a few overhead compared with original android system enabling full disk encryption.