Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture.The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(L.vanname...Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture.The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(L.vannamei) were investigated.This involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and gills.Three experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of 25.After 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels.Subsequently,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and 2.Shrimp fed with 40% protein at 25 in salinity(optimal conditions) were used as a control.Regardless of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the control.Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks.Ambient salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 weeks.These findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism.Increased dietary protein level could improve the osmoregulation capacity of L.vannamei with more energy resources allocated to GDH activity and expression.展开更多
Objective:The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.Methods:Human breast cancer cell line MCF-7 was transfected by C...Objective:The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.Methods:Human breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2.SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level,respectively.The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay,while cell cycle was measured by flow cytometry(FCM).Results:The plasmid could reduce the expression of Cyclin E.The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression.The transfected MCF-7 was arrested at G1 phase of cell cycle.Conclusion:The inhibition of Cyclin E can decrease the breast cancer cell's growth,increase its sensation to chemical therapy and slow down its cell cycle.The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.展开更多
The present study was undertaken to assess feeding effect on productive and reproductive performances of pre-natal and post-natal Red Chittagong Cattle （RCC） cows under on-station condition. A total of 16 pregnant R...The present study was undertaken to assess feeding effect on productive and reproductive performances of pre-natal and post-natal Red Chittagong Cattle （RCC） cows under on-station condition. A total of 16 pregnant RCC cows having pregnancy 6-7 months and between 1-3 parities was selected for the study and was allocated randomly into four dietary treatment groups. There are four type diets, i.e., To （standard diet according to National Research Council （NRC）, 1995）, T1 （5% below standard diet）, T2 （5% above standard diet）, T3 （10% above standard diet） and a control treatment T4 （maintained as farm practice）. The energy and protein requirements of experimental cows were determined as per standard developed by NRC. The results revealed that crude protein （CP） and metabolizable energy （ME, MJ/day） requirements among cows of different dietary groups were not significantly different, although CP requirements between T1 and T3 differed significantly. Total dry mater （DM） and ME intake differed significantly （P 〈 0.001） among cows of different dietary groups, but DM intake was significantly lower for cows in farm practice group Z4 compared to the cows of other dietary groups. The total CP intake had no significant variation （P 〉 0.05） among cows of all dietary groups, but CP intake from roughage varied significantly （P 〈 0.001）. Final live weight and total live weight gain had no significant variation for cows of all groups, but there were significant （P 〈 0.05） variation for daily weight gain. Daily weight gain of cows for first four dietary groups were significantly （P 〈 0.05） higher than cows of farm practice groups T4. Weight of calf from birth to 90 days and daily weight gain calves under different dietary groups showed that there were no significant （P 〉 0.05） variation of calf birth weights for all dietary groups, although lower birth weight was found in farm practice groups T4. Gestation length and postpartum heat period of cows of different dietary treatment groups revealed that there was no significant （P 〉 0.05） variation for those traits among cows of different dietary treatment groups, although there seems to be shorter postpartum heat for cows in dietary group T2 and longer period in farm practice group T4. Total and daily milk yield up to 30 days and 90 days of cows had no significant （P 〉 0.05） effect for different dietary treatment groups, although there seems to be slightly better milk production performance for dietary T2 and To, respectively for 30 days and 90 days total and daily milk yield. There were no significant （P 〉 0.05） changes of total and daily weight gain of cows for all dietary groups, however cows of standard dietary group To performed slightly better than those of cows of other dietary groups. Milk composition of cows of different dietary treatment groups showed that there was no significant （P 〉 0.05） variation on milk composition for cows of different dietary groups. Post-natal body condition score （BCS） for the 1st, 3rd and 4th months differed significantly （P 〈 0.05） among different dietary groups, while not found significant difference on the 2nd month. Therefore, it may be concluded that pre-natal feeding has significant effect on body weight gain, birth weight of calves and milk production of dairy cows. Hence, it is suggested that better nutrition may be provided during pre-natal period to harvest good quality of calves and higher milk yield from post-natal period.展开更多
Objective： The aim of our study was to analyze hepatic dysfunction and failure after hepatocellular carcinoma （HCC） resection and relationship of clinical and pathological factors. Methods： Clinical and pathologic...Objective： The aim of our study was to analyze hepatic dysfunction and failure after hepatocellular carcinoma （HCC） resection and relationship of clinical and pathological factors. Methods： Clinical and pathological data of 467 HCC patients was retrospectively reviewed, who underwent liver resection from January 2002 to December 2008 in the Affiliated Hospital of Medical College, Qingdao University, and the post-resectional liver dysfunction and failure risk factors were analyzed by univariate and multivariate analysis. Results： The morbidity of post-resectional liver dysfunction and failure was 1.7% and 2.1%. The post-resectional liver dysfunction and failure after HCC hepatectomy into the statistical analysis： univariate analysis revealed preoperative platelet level （〈 100 × 10^9）, serum albumin level （〈 35 g/L）, serum gamma-Glutamyl transferase （〉 64 U/L）, Child-Pugh classification （B）, MELD score （≥ 9）, intraoperative bleeding （-〉 1000 mL）, blood transfusion were positive factors, multivariate analysis （Logistic） revealed that preoperative platelet level （0.983, 95% CI = 0.971-0.995） and intraoperative blood transfusion （3.145, 95% CI = 1.027-12.028） were independent risk factors for post-resectional liver dysfunction and failure. Conclusion： Prevented liver failure and liver dysfunction occurring after liver resection, it is the key to accurate preoperative assessment of liver function and the patient＇s reserved liver functional, precise hepatectomy and reasonable blockage of hepatic inflow.展开更多
基金Supported by the National Natural Science Foundation of China(Nos. 31001098 and 30771670)the National High Technology R&D Program (863 Program) (No. 2006BAD01A13)+5 种基金the National Basic Research Program of China (973 Program) (No. 2009CB118702)Shanghai Committee of Science and Technology,China (Nos.08DZ1906401,09ZR1409800,10JC1404100)Shanghai Agriculture Science and Technology Key Grant (No.2-1,2009)the Specialized Research Fund for the Doctoral Program of Higher Education of China (No. 200802690012)partially by the E-Institute of Shanghai Municipal Education Commission (No. E03009)the Key and Open Laboratory of Marine and Estuarine Fisheries Resources and Ecology,Ministry of Agriculture
文摘Improvement in the osmoregulation capacity via nutritional supplies is vitally important in shrimp aquaculture.The effects of dietary protein levels on the osmoregulation capacity of the Pacific white shrimp(L.vannamei) were investigated.This involved an examination of growth performance,glutamate dehydrogenase(GDH) and Na+-K+ ATPase mRNA expression,,and GDH activity in muscles and gills.Three experimental diets were formulated,containing 25%,40%,and 50% dietary protein,and fed to the shrimp at a salinity of 25.After 20 days,no significant difference was observed in weight gain,though GDH and Na+-K+ ATPase gene expression and GDH activity increased with higher dietary protein levels.Subsequently,shrimp fed diets with 25% and 50% dietary protein were transferred into tanks with salinities of 38 and 5,respectively,and sampled at weeks 1 and 2.Shrimp fed with 40% protein at 25 in salinity(optimal conditions) were used as a control.Regardless of the salinities,shrimp fed with 50% dietary protein had significantly higher growth performance than other diets;no significant differences were found in comparison with the control.Shrimp fed with 25% dietary protein and maintained at salinities of 38 and 5 had significantly lower weight gain values after 2 weeks.Ambient salinity change also stimulated the hepatosomatic index,which increased in the first week and then recovered to a relatively normal level,as in the control,after 2 weeks.These findings indicate that in white shrimp,the specific protein nutrient and energy demands related to ambient salinity change are associated with protein metabolism.Increased dietary protein level could improve the osmoregulation capacity of L.vannamei with more energy resources allocated to GDH activity and expression.
基金Supported by a grant of National Natural Science Foundation of China(No. 30801116)
文摘Objective:The aim of the study was to investigate the biological character change of breast cancer cell line by inhibiting the Cyclin E expressing level.Methods:Human breast cancer cell line MCF-7 was transfected by Cyclin E siRNA vector pEGFP/CCNE2.SiRNA-induced silencing of Cyclin E was determined at RNA level and protein level,respectively.The proliferation of MCF-7 and its sensitivity to chemical therapy were measured by CCK-8 assay,while cell cycle was measured by flow cytometry(FCM).Results:The plasmid could reduce the expression of Cyclin E.The proliferation ability of MCF-7 was decreased and the sensitivity to chemical therapy was enhanced according to the inhibition of Cyclin E expression.The transfected MCF-7 was arrested at G1 phase of cell cycle.Conclusion:The inhibition of Cyclin E can decrease the breast cancer cell's growth,increase its sensation to chemical therapy and slow down its cell cycle.The Cyclin E siRNA may provide us a practical tool for further study on the gene therapy to breast cancers.
文摘The present study was undertaken to assess feeding effect on productive and reproductive performances of pre-natal and post-natal Red Chittagong Cattle （RCC） cows under on-station condition. A total of 16 pregnant RCC cows having pregnancy 6-7 months and between 1-3 parities was selected for the study and was allocated randomly into four dietary treatment groups. There are four type diets, i.e., To （standard diet according to National Research Council （NRC）, 1995）, T1 （5% below standard diet）, T2 （5% above standard diet）, T3 （10% above standard diet） and a control treatment T4 （maintained as farm practice）. The energy and protein requirements of experimental cows were determined as per standard developed by NRC. The results revealed that crude protein （CP） and metabolizable energy （ME, MJ/day） requirements among cows of different dietary groups were not significantly different, although CP requirements between T1 and T3 differed significantly. Total dry mater （DM） and ME intake differed significantly （P 〈 0.001） among cows of different dietary groups, but DM intake was significantly lower for cows in farm practice group Z4 compared to the cows of other dietary groups. The total CP intake had no significant variation （P 〉 0.05） among cows of all dietary groups, but CP intake from roughage varied significantly （P 〈 0.001）. Final live weight and total live weight gain had no significant variation for cows of all groups, but there were significant （P 〈 0.05） variation for daily weight gain. Daily weight gain of cows for first four dietary groups were significantly （P 〈 0.05） higher than cows of farm practice groups T4. Weight of calf from birth to 90 days and daily weight gain calves under different dietary groups showed that there were no significant （P 〉 0.05） variation of calf birth weights for all dietary groups, although lower birth weight was found in farm practice groups T4. Gestation length and postpartum heat period of cows of different dietary treatment groups revealed that there was no significant （P 〉 0.05） variation for those traits among cows of different dietary treatment groups, although there seems to be shorter postpartum heat for cows in dietary group T2 and longer period in farm practice group T4. Total and daily milk yield up to 30 days and 90 days of cows had no significant （P 〉 0.05） effect for different dietary treatment groups, although there seems to be slightly better milk production performance for dietary T2 and To, respectively for 30 days and 90 days total and daily milk yield. There were no significant （P 〉 0.05） changes of total and daily weight gain of cows for all dietary groups, however cows of standard dietary group To performed slightly better than those of cows of other dietary groups. Milk composition of cows of different dietary treatment groups showed that there was no significant （P 〉 0.05） variation on milk composition for cows of different dietary groups. Post-natal body condition score （BCS） for the 1st, 3rd and 4th months differed significantly （P 〈 0.05） among different dietary groups, while not found significant difference on the 2nd month. Therefore, it may be concluded that pre-natal feeding has significant effect on body weight gain, birth weight of calves and milk production of dairy cows. Hence, it is suggested that better nutrition may be provided during pre-natal period to harvest good quality of calves and higher milk yield from post-natal period.
文摘Objective： The aim of our study was to analyze hepatic dysfunction and failure after hepatocellular carcinoma （HCC） resection and relationship of clinical and pathological factors. Methods： Clinical and pathological data of 467 HCC patients was retrospectively reviewed, who underwent liver resection from January 2002 to December 2008 in the Affiliated Hospital of Medical College, Qingdao University, and the post-resectional liver dysfunction and failure risk factors were analyzed by univariate and multivariate analysis. Results： The morbidity of post-resectional liver dysfunction and failure was 1.7% and 2.1%. The post-resectional liver dysfunction and failure after HCC hepatectomy into the statistical analysis： univariate analysis revealed preoperative platelet level （〈 100 × 10^9）, serum albumin level （〈 35 g/L）, serum gamma-Glutamyl transferase （〉 64 U/L）, Child-Pugh classification （B）, MELD score （≥ 9）, intraoperative bleeding （-〉 1000 mL）, blood transfusion were positive factors, multivariate analysis （Logistic） revealed that preoperative platelet level （0.983, 95% CI = 0.971-0.995） and intraoperative blood transfusion （3.145, 95% CI = 1.027-12.028） were independent risk factors for post-resectional liver dysfunction and failure. Conclusion： Prevented liver failure and liver dysfunction occurring after liver resection, it is the key to accurate preoperative assessment of liver function and the patient＇s reserved liver functional, precise hepatectomy and reasonable blockage of hepatic inflow.
