An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home ...An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home built HR ESI TOF MS was applied to detect fat soluble components in Danshen( Salvia miltiorrhiza Bage ) directly. Compared with the method of HPLC ESI MS(High Performance Liquid Chromatograph Electrospray ionization Mass Spectrometry) method, HR ESI TOF MS method is more accurate, quick, simple and reliable, so the method can become an effective tool for the quality control of TCM.展开更多
Paecilomyces is a genus closely related to Penicillium. Most species of Paecilomyces are common soilborne saprobes, a section in this genus is known primarily as parasites of insects. Some species of another section a...Paecilomyces is a genus closely related to Penicillium. Most species of Paecilomyces are common soilborne saprobes, a section in this genus is known primarily as parasites of insects. Some species of another section are quite tolerant of high temperatures.展开更多
Aldo-keto reductase 1D1(AKR1D1) deficiency,a rare but life-threatening form of bile acid deficiency,has not been previously described in China.Here,we describe the first two primary 4-3-oxosteroid 5β-reductase defici...Aldo-keto reductase 1D1(AKR1D1) deficiency,a rare but life-threatening form of bile acid deficiency,has not been previously described in China.Here,we describe the first two primary 4-3-oxosteroid 5β-reductase deficiency patients in China's Mainland diagnosed by fast atom bombardment-mass spectroscopy of urinary bile acids and confirmed by genetic analysis.A high proportion of atypical 3-oxo-4-bile acids in the urine indicated a deficiency in 4-3-oxosteroid 5β-reductase.All of the coding exons and adjacent intronic sequence of the AKR1D1 gene were sequenced using peripheral lymphocyte genomic DNA of two patients and one of the patient's parents.One patient exhibited compound heterozygous mutations:c.396C>A and c.722A>T,while the other was heterozygous for the mutation c.797G>A.Based on these mutations,a diagnosis of primary 4-3-oxosteroid 5β-reductase deficiency could be confirmed.With ursodeoxycholic acid treatment and fat-soluble vitamin supplements,liver function tests normalized rapidly,and the degree of hepatomegaly was markedly reduced in both patients.展开更多
文摘An excellent analytical tool for the separation and analysis of TCM(Traditional Chinese Medicine) by using HR ESI TOF MS(High Resolution Electrospray Ionization Time of flight Mass Spectrometry) was developed. A home built HR ESI TOF MS was applied to detect fat soluble components in Danshen( Salvia miltiorrhiza Bage ) directly. Compared with the method of HPLC ESI MS(High Performance Liquid Chromatograph Electrospray ionization Mass Spectrometry) method, HR ESI TOF MS method is more accurate, quick, simple and reliable, so the method can become an effective tool for the quality control of TCM.
基金Supported by industrial fementation lab. in Yunnan University and National Natural Science Foundation of China (No. 30270011)
文摘Paecilomyces is a genus closely related to Penicillium. Most species of Paecilomyces are common soilborne saprobes, a section in this genus is known primarily as parasites of insects. Some species of another section are quite tolerant of high temperatures.
基金Supported by National Natural Science Foundation of China, No.81070281
文摘Aldo-keto reductase 1D1(AKR1D1) deficiency,a rare but life-threatening form of bile acid deficiency,has not been previously described in China.Here,we describe the first two primary 4-3-oxosteroid 5β-reductase deficiency patients in China's Mainland diagnosed by fast atom bombardment-mass spectroscopy of urinary bile acids and confirmed by genetic analysis.A high proportion of atypical 3-oxo-4-bile acids in the urine indicated a deficiency in 4-3-oxosteroid 5β-reductase.All of the coding exons and adjacent intronic sequence of the AKR1D1 gene were sequenced using peripheral lymphocyte genomic DNA of two patients and one of the patient's parents.One patient exhibited compound heterozygous mutations:c.396C>A and c.722A>T,while the other was heterozygous for the mutation c.797G>A.Based on these mutations,a diagnosis of primary 4-3-oxosteroid 5β-reductase deficiency could be confirmed.With ursodeoxycholic acid treatment and fat-soluble vitamin supplements,liver function tests normalized rapidly,and the degree of hepatomegaly was markedly reduced in both patients.
