采用平板透明圈法从不同来源土壤样品中分离得到31株脂肪酶生产菌株，经复筛得到一株脂肪酶产量较高的菌株Mhy-1。根据培养特征、生理生化分析及16SrDNA序列同源性分析，初步鉴定菌株Mhy一1为杀鲑气单胞菌（Aeroraonas salmonicida），...采用平板透明圈法从不同来源土壤样品中分离得到31株脂肪酶生产菌株，经复筛得到一株脂肪酶产量较高的菌株Mhy-1。根据培养特征、生理生化分析及16SrDNA序列同源性分析，初步鉴定菌株Mhy一1为杀鲑气单胞菌（Aeroraonas salmonicida），该菌的最适生长温度及初始pH分别为35℃和pH7．0，最适产酶温度及初始pH分别为30℃和pH9．0。采用硫酸铵盐析、DEAE—Sepharose fast flow阴离子交换层析从菌株Mhy-1的发酵上清液中分离纯化了脂肪酶，酶的分子量约为34ku，最适作用温度为45qC，最适作用pH8．0。该酶在pH7．0—9．0和温度30—70℃的范围内稳定。在70℃条件下能维持80％以上酶活力，是一热稳定酶，纯化后的酶可用于生物柴油的催化合成。展开更多
The interest for lipase production is due to the ability of this enzyme to catalyze some reactions, such as the transesterification. Although industrial biodiesel is produced chemically, there are several problems ass...The interest for lipase production is due to the ability of this enzyme to catalyze some reactions, such as the transesterification. Although industrial biodiesel is produced chemically, there are several problems associated with this technology that can be prevented through the use of lipases. The present work aimed to select microorganisms with potential for production of lipase with transesterification activity. The lipase from Burkholderia cepacia was the one with the most promising results for this type of reaction, showing results of hydrolytic activity at 37 ℃and pH 8.0. The pH and volume of crude enzyme extract that showed favorable for synthesis ofbiodiesel is at about pH 6.0 and 3.75 mL, respectively, which represents approximately 42% of water in the system, ensuring the conversion of nearly 60% to biodiesel.展开更多
文摘采用平板透明圈法从不同来源土壤样品中分离得到31株脂肪酶生产菌株，经复筛得到一株脂肪酶产量较高的菌株Mhy-1。根据培养特征、生理生化分析及16SrDNA序列同源性分析，初步鉴定菌株Mhy一1为杀鲑气单胞菌（Aeroraonas salmonicida），该菌的最适生长温度及初始pH分别为35℃和pH7．0，最适产酶温度及初始pH分别为30℃和pH9．0。采用硫酸铵盐析、DEAE—Sepharose fast flow阴离子交换层析从菌株Mhy-1的发酵上清液中分离纯化了脂肪酶，酶的分子量约为34ku，最适作用温度为45qC，最适作用pH8．0。该酶在pH7．0—9．0和温度30—70℃的范围内稳定。在70℃条件下能维持80％以上酶活力，是一热稳定酶，纯化后的酶可用于生物柴油的催化合成。
文摘The interest for lipase production is due to the ability of this enzyme to catalyze some reactions, such as the transesterification. Although industrial biodiesel is produced chemically, there are several problems associated with this technology that can be prevented through the use of lipases. The present work aimed to select microorganisms with potential for production of lipase with transesterification activity. The lipase from Burkholderia cepacia was the one with the most promising results for this type of reaction, showing results of hydrolytic activity at 37 ℃and pH 8.0. The pH and volume of crude enzyme extract that showed favorable for synthesis ofbiodiesel is at about pH 6.0 and 3.75 mL, respectively, which represents approximately 42% of water in the system, ensuring the conversion of nearly 60% to biodiesel.
