Zinc finger in the cerebellum 1 （Zicl） is known to regulate neurogenesis and myogenesis in the develop- mental stage and widely used as one of the brown adipocyte-specific markers. In this study, we examined the eff...Zinc finger in the cerebellum 1 （Zicl） is known to regulate neurogenesis and myogenesis in the develop- mental stage and widely used as one of the brown adipocyte-specific markers. In this study, we examined the effect of Zicl on brown adipogenesis. Overexpression of Zicl attenuated the lipid accumulation and the expressions of PPAR72 and C/EBPα in C3H10T1/2 mesenchymal stem cells. The mRNA levels of BAT-specific thermogenic genes （PRDM16, PGC-1α and UCP1） and fatty acid oxidation regulatory genes （PPARα, CPT1α, CPT1β and COX7α1） were suppressed in Zicl-overexpressed cells. Moreover, overexpression of Zicl reduced the mitochondrial oxidative phosphorylation （OXPHOS） regulatory proteins including ATP5α, UQCRC2, SDHB and NDUFB5. These results indicate a potential role of Zicl in the regulation of brown adipogenesis via inhibiting adipogenesis, fatty acid oxidation and mitochondrial OXPHOS.展开更多
基金supported by the Strategic Priority Research Program(XDB13030000)Key Research Program(KJZD-EW-L01-3)+1 种基金the One Hundred Talents Program of the Chinese Academy of Sciences and the Ministry of Science and Technology of China(2012CBA01301 and 2012CB944701,respectively)the National Natural Science Foundation of China(31171131,81370951)
文摘Zinc finger in the cerebellum 1 （Zicl） is known to regulate neurogenesis and myogenesis in the develop- mental stage and widely used as one of the brown adipocyte-specific markers. In this study, we examined the effect of Zicl on brown adipogenesis. Overexpression of Zicl attenuated the lipid accumulation and the expressions of PPAR72 and C/EBPα in C3H10T1/2 mesenchymal stem cells. The mRNA levels of BAT-specific thermogenic genes （PRDM16, PGC-1α and UCP1） and fatty acid oxidation regulatory genes （PPARα, CPT1α, CPT1β and COX7α1） were suppressed in Zicl-overexpressed cells. Moreover, overexpression of Zicl reduced the mitochondrial oxidative phosphorylation （OXPHOS） regulatory proteins including ATP5α, UQCRC2, SDHB and NDUFB5. These results indicate a potential role of Zicl in the regulation of brown adipogenesis via inhibiting adipogenesis, fatty acid oxidation and mitochondrial OXPHOS.
