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脂肪酸组分分析在不动杆菌鉴定中的应用 被引量:10
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作者 张晓霞 王直强 +2 位作者 李世贵 顾金刚 姜瑞波 《生物技术通报》 CAS CSCD 北大核心 2009年第6期151-154,共4页
以不动杆菌属(Acinetobacter)中的A.baumannii、A.haemolyticus、A.johnsonii、A.junii、A.lwoffii模式菌株为参比菌株,对中国农业微生物菌种保藏管理中心(ACCC)所保藏的6株不动杆菌进行脂肪酸组分分析及16S rRNA基因系统进化分析。结... 以不动杆菌属(Acinetobacter)中的A.baumannii、A.haemolyticus、A.johnsonii、A.junii、A.lwoffii模式菌株为参比菌株,对中国农业微生物菌种保藏管理中心(ACCC)所保藏的6株不动杆菌进行脂肪酸组分分析及16S rRNA基因系统进化分析。结果表明,利用脂肪酸分析可将6株菌完全准确地鉴定到属的水平上,这一点与16S rRNA基因分析结果一致;在种的水平上,16S rRNA基因进化分析与脂肪酸组分分析的结果可互为补充,相互印证。同时,通过对不动杆菌部分模式菌株及供试菌株的脂肪酸组分分析,报道了不动杆菌的特征性脂肪酸为C18:1 w9c、C16:00和C16:1w7c/C16:1w6c。 展开更多
关键词 不动杆菌 脂肪酸组分分析 16SrDNA系统进化分析
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Inheritance of Fat and Fatty Acid Compositions in Peanut (Arachis hypogaea L.) 被引量:5
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作者 张新友 祝水金 +6 位作者 韩锁义 徐静 刘华 汤丰收 董文召 臧秀旺 张忠信 《Agricultural Science & Technology》 CAS 2011年第7期943-946,974,共5页
[Objective] The aim was to explore the inheritance of fat and fatty acid compositions in peanut.[Method] Genetic and correlation analyses were performed using a mixed genetic model of major gene plus polygene with 215... [Objective] The aim was to explore the inheritance of fat and fatty acid compositions in peanut.[Method] Genetic and correlation analyses were performed using a mixed genetic model of major gene plus polygene with 215 F9 RILs and their parents ZHENG 8903 and YUHUA No.4.[Result] The optimal genetic model of the fat content was two linked complementary major gene plus polygene inheritance model.The heritability of major genes was 22.88%.Oleic,linoleic,and palmitic acid content were controlled by two major genes and the heritability was 75.11%,75.00% and 60.95%,respectively.Stearic and arachidic acid content were controlled by two major genes plus polygenes,the heritability of major genes was 26.43% and 33.17%,respectively.O/L ratio and behenic acid content were controlled by three major genes,the heritability was 70.65% and 30.70%,respectively.[Conclusion] The result in this study was that accumulating polygenes should be emphasized in the improvement of fat content,while utilization of major genes is enhanced in improvement of oleic and linoleic acids. 展开更多
关键词 PEANUT FAT Fatty acid composition INHERITANCE
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一种准确、简便测定CRISPR/Cas9基因编辑效率的方法 被引量:10
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作者 朱丽颖 郑月萍 +2 位作者 徐雪珍 段芊芊 韩妮莎 《江苏农业学报》 CSCD 北大核心 2020年第2期299-305,共7页
CRISPR/Cas9基因编辑技术是近年来发展迅速的一项基因定点编辑技术,但对特定CRISPR/Cas9基因编辑技术的编辑效率进行有效评估的方法仍十分匮乏,本研究的目的是建立一种准确、简便测定CRISPR/Cas9基因编辑效率的方法。在我们的方法中,将... CRISPR/Cas9基因编辑技术是近年来发展迅速的一项基因定点编辑技术,但对特定CRISPR/Cas9基因编辑技术的编辑效率进行有效评估的方法仍十分匮乏,本研究的目的是建立一种准确、简便测定CRISPR/Cas9基因编辑效率的方法。在我们的方法中,将与种子专一表达启动子(2S3)连接的mCherry荧光蛋白报告基因作为选择基因,并以编码脂肪酸脱氢酶FAD2的基因为靶序列;然后对经CRISPR/Cas9编辑的拟南芥种子进行脂肪酸组分分析,根据其组分变化计算该方法的编辑效率。结果显示,利用mCherry荧光蛋白报告基因的种子专一表达特性,可简便快速地筛选阳性的初级转化子和后代的无转基因突变子;而种子油脂分析法能快速、准确地鉴定CRISPR/Cas9编辑的突变体,可以精确地检测出核酸长度小至单个碱基对的突变,用该方法获得的CRISPR/Cas9基因编辑效率(54.5%~74.1%)远高于基于聚丙烯酰氨凝胶电泳法获得的编辑效率(3.7%~15.4%)。说明,该方法检测CRISPR/Cas9基因编辑效率的高效性与可行性。 展开更多
关键词 CRISPR/Cas9 基因编辑 突变体筛选 脂肪酸组分分析
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Analysis of fatty acid composition of sea cucumber Apostichopus japonicus using multivariate statistics 被引量:2
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作者 徐勤增 高菲 +1 位作者 许强 杨红生 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 2014年第6期1314-1319,共6页
Fatty acids(FAs) provide energy and also can be used to trace trophic relationships among organisms.Sea cucumber Apostichopus japonicus goes into a state of aestivation during warm summer months.We examined fatty acid... Fatty acids(FAs) provide energy and also can be used to trace trophic relationships among organisms.Sea cucumber Apostichopus japonicus goes into a state of aestivation during warm summer months.We examined fatty acid profiles in aestivated and non-aestivated A.japonicus using multivariate analyses(PERMANOVA,MDS,ANOSIM,and SIMPER).The results indicate that the fatty acid profiles of aestivated and non-aestivated sea cucumbers differed significantly.The FAs that were produced by bacteria and brown kelp contributed the most to the differences in the fatty acid composition of aestivated and nonaestivated sea cucumbers.Aestivated sea cucumbers may synthesize FAs from heterotrophic bacteria during early aestivation,and long chain FAs such as eicosapentaenoic(EPA) and docosahexaenoic acid(DHA) that produced from intestinal degradation,are digested during deep aestivation.Specific changes in the fatty acid composition of A.japonicus during aestivation needs more detailed study in the future. 展开更多
关键词 fatty acid (FAs) Apostichopusjaponicus AESTIVATION multivariate analysis
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