为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,...为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.展开更多
Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alt...Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alternative for generating bioproducts with a higher added value through bioconversion by microorganisms. The aim of this study was to screen parameters, such as pH （4, 5, 6, 7 and 8） and the initial glycerol concentration at 30 ± ℃ with agitation at 150 rpm for bioemulsifier and lipid synthesis in a submerged medium by Yarrowia lipolytica IMUFRJ 50678 from crude glycerin. The best conditions for bioemulsifier production were 30 ± ℃ at pH： 6 with 50 g/L of initial substrate, which produced 2.7 g/L of lipids, from which the optimum 300.5 mg/L of triglycerides was produced over 48 h of microorganism growth.展开更多
文摘为研究多基因调控下微藻油脂合成调控的分子机制,对acs2-cis1-dof基因共调控的莱茵衣藻藻株DLC进行转录组测序及分析,获得749个差异表达基因.通过京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)聚类分析发现,有51个差异表达基因参与碳水化合物代谢,15个差异表达基因参与脂质代谢,差异基因主要富集在甘油酯代谢、脂肪酸降解、糖酵解及糖异生、淀粉和蔗糖代谢等通路中.采用BODIPY 505/515荧光染色法检测发现,热激后莱茵衣藻藻株DLC细胞内的甘油三酯质量较野生型提高了193%,通过检测吸光度发现藻细胞的淀粉质量降低了45%,说明基因共调控不仅增强了脂质合成代谢,还使得糖代谢中的碳流重新定向.研究工作为探究基因共调控下脂质合成代谢的分子机理以及微藻产油基因工程提供理论参考.
文摘Transesterification is the most common production process for biodiesel. From this reaction, a glycerin phase is produced that is impure, thus lowering market value. However, because it is rich in carbon, it is an alternative for generating bioproducts with a higher added value through bioconversion by microorganisms. The aim of this study was to screen parameters, such as pH （4, 5, 6, 7 and 8） and the initial glycerol concentration at 30 ± ℃ with agitation at 150 rpm for bioemulsifier and lipid synthesis in a submerged medium by Yarrowia lipolytica IMUFRJ 50678 from crude glycerin. The best conditions for bioemulsifier production were 30 ± ℃ at pH： 6 with 50 g/L of initial substrate, which produced 2.7 g/L of lipids, from which the optimum 300.5 mg/L of triglycerides was produced over 48 h of microorganism growth.
