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利用小鼠骨骼肌细胞持续表达单链胰岛素类似物进行1型糖尿病模型鼠的长效基因治疗 被引量:1
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作者 杨平 苏若珂 +2 位作者 邓璐 杨月瑶 王刚 《四川动物》 北大核心 2019年第6期607-615,共9页
目的探讨Pluronic L64-电脉冲(L/E)体系介导的单链胰岛素类似物基因在骨骼肌组织的传递表达对1型糖尿病小鼠的治疗效果和影响。方法将胰岛素基因中的C肽区域换成(GGGGS)3,克隆到pcDNA3.1(+)质粒中,构建载体pcDNA-INS。使用链脲佐菌素腹... 目的探讨Pluronic L64-电脉冲(L/E)体系介导的单链胰岛素类似物基因在骨骼肌组织的传递表达对1型糖尿病小鼠的治疗效果和影响。方法将胰岛素基因中的C肽区域换成(GGGGS)3,克隆到pcDNA3.1(+)质粒中,构建载体pcDNA-INS。使用链脲佐菌素腹腔注射雄性BALB/c小鼠建立1型糖尿病小鼠模型,并分为L/E联合pcDNA3.1(+)空质粒治疗的L/E-pcDNA3.1(+)组、仅使用pcDNA-INS裸质粒治疗的pcDNA-INS组和L/E联合pcDNA-INS裸质粒治疗的L/E-pcDNA-INS组。模型小鼠在胫骨前肌注射相关质粒进行治疗,且根据治疗方案在注射1 h后进行Pluronic L64-电脉冲处理,并以生理盐水处理的正常小鼠(NC)组作为对照。记录各组小鼠的血糖、体质量、血清胰岛素含量、生存率、免疫组化、外形特征及组织病理等指标。结果 (1)pcDNA-INS质粒通过酶切和测序鉴定无误;(2)与NC组相比,模型小鼠血糖显著上升,体质量显著下降(P<0.05),且胰岛遭到严重破坏;(3)L/E-pcDNA-INS组的血糖水平和血清胰岛素含量显著缓解,与NC组较为接近,pcDNA-INS组的血糖缓解和胰岛素补偿远不及L/E-pcDNA-INS组(P<0.05),且与NC组之间的差异一直有统计学意义(P<0.05)。第4周免疫组化结果显示,L/E-pcDNA-INS组的胰腺胰岛素表达较少,但肌肉胰岛素表达量较高,且该组生存率较高,与NC组均为100%(12/12),L/E-pcDNA-INS组的病理结果和外形特征与NC组小鼠最为接近,L/E-pcDNA3.1(+)组与pcDNA-INS组在不同部位均有不同程度的病变发生,且鼠毛疏少粗糙,体形较小。结论 Pluronic L64-电脉冲体系与单链胰岛素类似物基因联合治疗1型糖尿病的效果较好,且无明显不良影响,可为相关研究提供实验依据。 展开更多
关键词 胰岛素类似物 PLURONIC L64-电脉冲体系 1型糖尿病小鼠 骨骼肌基因治疗
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Design of ERT system 被引量:1
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作者 朱建平 王保良 +1 位作者 黄志尧 李海青 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 SCIE EI CAS CSCD 2005年第12期1446-1448,共3页
A new electrical resistance tomography (ERT) system, based on the bi-directional current pulse technique, is de- veloped. Polarization effects at the electrodes are eliminated. During each half cycle of the excitation... A new electrical resistance tomography (ERT) system, based on the bi-directional current pulse technique, is de- veloped. Polarization effects at the electrodes are eliminated. During each half cycle of the excitation process, direct current (DC) is used as the excitation source and filtering is not needed. Moreover, using optimized data collection protocol, the number of current injections for a 16-electrode system is reduced to 14 for 104 independent readings of an image. Experimental results showed that the system is effective using the sensitivity coefficient algorithm for image reconstruction. 展开更多
关键词 Electrical resistance tomography (ERT) Direct current (DC) EXCITATION RECONSTRUCTION
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A ground reflection model for I-UWB signal propagation
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作者 汪洋 张钦宇 +2 位作者 张乃通 刘法 张继良 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 2008年第1期23-26,共4页
Impulse systems do not undergo the multi-path destructive interference that manifests itself as Rayleigh fading prevalent in continuous wave systems, but rather show up as delayed replications of the direct impulse. I... Impulse systems do not undergo the multi-path destructive interference that manifests itself as Rayleigh fading prevalent in continuous wave systems, but rather show up as delayed replications of the direct impulse. In this paper, the model for impulse signals propagation over a plane earth is proposed. The condition for direct pulse and its replication overlapping each other was investigated. The model described here also takes into account the polarization of the transmission signals and the reflection coefficient of the plane, which was always neglected by previous approaches. The simulation result shows that the path loss can be characterized as 3 zones with different path loss exponents as the distance between transmitter and receiver (T-R) increasing. 展开更多
关键词 UWB path loss 2-ray model muhipath
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Impulsive Synchronization of Laser Plasma System
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作者 LI Yang LIAO Xiao-Feng LI Chuan-Dong CHEN Guo 《Communications in Theoretical Physics》 SCIE CAS CSCD 2007年第6期1067-1072,共6页
The issue of impulsive synchronization of the coupled chaotic laser plasma system is investigated. A new framework for impulsive synchronization of such chaotic systems is presented, which makcs the synchronization er... The issue of impulsive synchronization of the coupled chaotic laser plasma system is investigated. A new framework for impulsive synchronization of such chaotic systems is presented, which makcs the synchronization error system a linear impulsive control system. We derive some suffcient conditions for the synchronization of a laser plasma system via impulsive control with the varying impulsive intervals, which allows us to derive the impulsive synchronization law easily. To illustrate the effectiveness of the proposed results, two numerical examples are given. 展开更多
关键词 CHAOS impulsive stabilization impulsive control impulsive synchronization
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国际主要股票市场的波动风险溢出效应及其动态演绎过程研究 被引量:2
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作者 周东海 陈滨霞 蒋远营 《数理统计与管理》 CSSCI 北大核心 2022年第5期927-950,共24页
从已实现波动溢出的角度考察金融市场风险传染机制,可追溯高频、高阶矩金融风险在国际金融市场中的传染途径与溢出方向。本文先从“传统经济基础理论”、“金融风险传染猜想”两个层面分析股市波动风险的溢出效应。接着引入时变参数(TVP... 从已实现波动溢出的角度考察金融市场风险传染机制,可追溯高频、高阶矩金融风险在国际金融市场中的传染途径与溢出方向。本文先从“传统经济基础理论”、“金融风险传染猜想”两个层面分析股市波动风险的溢出效应。接着引入时变参数(TVP)因子和随机波动(SV)因子,从时差效应和信息冲击效应等角度对美国、日本、香港、沪市的日内已实现波动率(RV)时间序列数据进行建模。通过多项稳健性检验发现,证券市场之间的波动具有显著的风险溢出效应和金融传染效应,且存在时变性与结构突变特征。在全球风险传递链中,美国市场在波动传递中占据主导地位,冲击会迅速外溢到其他市场,其中波动风险会最大程度影响到日本。中国内地股市不仅对外围市场波动信息反应迅速而强烈,而且能够显著影响外围市场。中国香港是亚洲股票市场波动外溢的中心地区之一,与内地股市之间的短期双向溢出效应最为显著。本文新提出的时变脉冲函数体系,对市场风险传染有很强的全局刻画能力,特别是在港股跃居全球股市前三甲的预判中取得了很满意结论。时变脉冲体系可以推广应用到金融市场和宏观计量经济等的多个领域。 展开更多
关键词 已实现波动 溢出效应 时变参数VAR模型 时变脉冲体系 稳健性检验
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