Pyropia haitanensis has prominent stress-resistance characteristics and is endemic to China. Studies into the stress responses in these algae could provide valuable information on the stress-response mechanisms in the...Pyropia haitanensis has prominent stress-resistance characteristics and is endemic to China. Studies into the stress responses in these algae could provide valuable information on the stress-response mechanisms in the intertidal Rhodophyta. Here, the effects of salinity and light intensity on the quantum yield of photosystem II in Py. haitanensis were investigated using pulse-amplitude-modulation fluorometry. Total RNA and genomic DNA of the samples under different stress conditions were isolated. By normalizing to the genomic DNA quantity, the RNA content in each sample was evaluated. The cDNA was synthesized and the expression levels of seven potential internal control genes were evaluated using qRT-PCR method. Then, we used geNorm, a common statistical algorithm, to analyze the qRT-PCR data of seven reference genes. Potential genes that may constantly be expressed under different conditions were selected, and these genes showed stable expression levels in samples under a salinity treatment, while tubulin, glyceraldehyde- 3-phosphate dehydrogenase and actin showed stability in samples stressed by strong light. Based on the results of the pulse amplitude-modulation fluorometry, an absolute quantification was performed to obtain gene copy numbers in certain stress-treated samples. The stably expressed genes as determined by the absolute quantification in certain samples conformed to the results of the geNorm screening. Based on the results of the software analysis and absolute quantification, we proposed that elongation factor 3 and 18S ribosomal RNA could be used as internal control genes when the Py. haitanensis blades were subjected to salinity stress, and that a-tubulin and 18S ribosomal RNA could be used as the internal control genes when the stress was from strong light. In general, our findings provide a convenient reference for the selection of internal control genes when designing experiments related to stress responses in Py. haitanensis.展开更多
As a new three-dimensional(3-D)modulation,Polarization Quadrature Amplitude Modulation(PQAM) can be regarded as the combination of Pulse amplitude modulation(PAM) and Quadrature Amplitude Modulation(QAM) Modulation.It...As a new three-dimensional(3-D)modulation,Polarization Quadrature Amplitude Modulation(PQAM) can be regarded as the combination of Pulse amplitude modulation(PAM) and Quadrature Amplitude Modulation(QAM) Modulation.It can better improve the digital communication efficiency and reduce the Symbol error rate(SER) of the system than one-dimensional or two-dimensional modulation scheme.How to design a feasible constellation is the most concerned problem of PQAM currently.This paper first studies the relationship between the SER theoretical value of PQAM and the distribution of M and N,proposes a new M,N allocation scheme.Secondly,a new and straightforward design method of constructing higher-level 3-D signal constellations,which can be matched with the PQAM,and the constellation can divided into three different structures according to the ary for PQAM.Finally,the simulation results show that:in PQAM system,the modulation scheme and the constellation mapping scheme are proposed in this paper which can effectively reduce the system SER and improve the anti-noise performance of the system.展开更多
Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for...Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.展开更多
Based on the proposed partly equidifferent mapping and its specific Differential Amplitude and Pulse Position Modulation(DAPPM) demodulation, a modified FSO scheme for turbulent channel is designed and analyzed. The n...Based on the proposed partly equidifferent mapping and its specific Differential Amplitude and Pulse Position Modulation(DAPPM) demodulation, a modified FSO scheme for turbulent channel is designed and analyzed. The novel Low Density Parity Check(LDPC) coded 4×4 and 4×8 DAPPM Free-Space Optical communication(FSO) system is constructed. The Monte Carlo simulation results show approximately 2d B transmit power reduction against classical LDPC-DAPPM at the identical Bit-Error-Rate in strong turbulent channel. The proposed partly equidifferent mapping is compatible with other modulations, so it enables widespread adoption in other coded FSO systems.展开更多
Over the last decades, the usage of PAM （pulse amplitude modulated） fluorometers for assessment of chlorophyll-a fluorescence variations became widely applied on marine macroalgae physiology and ecophysiology resear...Over the last decades, the usage of PAM （pulse amplitude modulated） fluorometers for assessment of chlorophyll-a fluorescence variations became widely applied on marine macroalgae physiology and ecophysiology researches. Due to the increased use of these methods, a large number of studies, mainly relating to macroalgae ecology and physiology were worldwide reported. In this context, it was also created a mismatch of concepts about fluorescence of the chlorophyll-a and its application. Under this background, this study compile and summarize the state of the art knowledge regarding to the chlorophyll fluorescence, contextualizing the use of the PAM method with the main factors regulating photosynthesis （light, temperature, salinity nitrogen and phosphorus） in marine macroalgae. Moreover, this study also references the most used terms and shows some examples found in literature about the applicability of fluorescence parameters. The herein findings and the discussed examples, helps to emphasize the importance of fluorescence usage, that highlights the understanding of photosynthetic responses in macroalgal physiology and ecology.展开更多
Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for power...Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.展开更多
A cost-effective ultra-dense wavelength-division-multiplexed passive optical network(UD-WDM PON) with speed of 12.5 Gbit/s and channel spacing of 12.5 GHz is proposed and demonstrated. The distributed feedback(DFB) la...A cost-effective ultra-dense wavelength-division-multiplexed passive optical network(UD-WDM PON) with speed of 12.5 Gbit/s and channel spacing of 12.5 GHz is proposed and demonstrated. The distributed feedback(DFB) lasers modulated in 4-level pulse amplitude modulation(4-PAM) format are used for downstream links, and the reflective semiconductor optical amplifiers(RSOAs) together with an optical frequency comb modulated in quadrature phase shift keying(QPSK) format are used for upstream links. We can achieve the error-free transmission of the upstream signals with speed of 12.5 Gbit/s even after 20 km single-mode fiber(SMF). The power penalty obtained by using the frequency comb generator instead of a tunable laser is around 0.5 d B. By using 11 DFB lasers and a set of intensity and phase modulators, it is possible to provide the seed light for 297 optical network units(ONUs) within the C-band.展开更多
基金Supported by the National Natural Science Foundation of China(Nos.41476140,41306151,41676157,41506172)the Strategic Leading Science and Technology Projects of Chinese Academy of Sciences(No.XDA11020404)+1 种基金the China Postdoctoral Science Foundation(No.2015M582153)the Science and Technology Plan of Jiangsu Province(No.BE2016330)
文摘Pyropia haitanensis has prominent stress-resistance characteristics and is endemic to China. Studies into the stress responses in these algae could provide valuable information on the stress-response mechanisms in the intertidal Rhodophyta. Here, the effects of salinity and light intensity on the quantum yield of photosystem II in Py. haitanensis were investigated using pulse-amplitude-modulation fluorometry. Total RNA and genomic DNA of the samples under different stress conditions were isolated. By normalizing to the genomic DNA quantity, the RNA content in each sample was evaluated. The cDNA was synthesized and the expression levels of seven potential internal control genes were evaluated using qRT-PCR method. Then, we used geNorm, a common statistical algorithm, to analyze the qRT-PCR data of seven reference genes. Potential genes that may constantly be expressed under different conditions were selected, and these genes showed stable expression levels in samples under a salinity treatment, while tubulin, glyceraldehyde- 3-phosphate dehydrogenase and actin showed stability in samples stressed by strong light. Based on the results of the pulse amplitude-modulation fluorometry, an absolute quantification was performed to obtain gene copy numbers in certain stress-treated samples. The stably expressed genes as determined by the absolute quantification in certain samples conformed to the results of the geNorm screening. Based on the results of the software analysis and absolute quantification, we proposed that elongation factor 3 and 18S ribosomal RNA could be used as internal control genes when the Py. haitanensis blades were subjected to salinity stress, and that a-tubulin and 18S ribosomal RNA could be used as the internal control genes when the stress was from strong light. In general, our findings provide a convenient reference for the selection of internal control genes when designing experiments related to stress responses in Py. haitanensis.
基金supported in part by the National Natural Science Foundation of China (61561039, 61271177, and 61461044)
文摘As a new three-dimensional(3-D)modulation,Polarization Quadrature Amplitude Modulation(PQAM) can be regarded as the combination of Pulse amplitude modulation(PAM) and Quadrature Amplitude Modulation(QAM) Modulation.It can better improve the digital communication efficiency and reduce the Symbol error rate(SER) of the system than one-dimensional or two-dimensional modulation scheme.How to design a feasible constellation is the most concerned problem of PQAM currently.This paper first studies the relationship between the SER theoretical value of PQAM and the distribution of M and N,proposes a new M,N allocation scheme.Secondly,a new and straightforward design method of constructing higher-level 3-D signal constellations,which can be matched with the PQAM,and the constellation can divided into three different structures according to the ary for PQAM.Finally,the simulation results show that:in PQAM system,the modulation scheme and the constellation mapping scheme are proposed in this paper which can effectively reduce the system SER and improve the anti-noise performance of the system.
文摘Since the poor performance of orthogonal binary Pulse Position Modulation (PPM) compared with binary Pulse Amplitude Modulation (PAM), this paper presents a new modulation scheme named Pulse Width Modulation (PWM) for Impulse Radio Ultra-WideBand (IR-UWB) communication systems. This modulation scheme uses pulses with equal amplitude and different widths to carry different information. The receiver employs differences between similarity coefficients among these pulses to distinguish different information. Both theoretical analysis and simulation results verify that this novel scheme has a Signal to Noise Ratio (SNR) gain of about 1.75 dB compared with or- thogonal binary PPM, and has an SNR loss of about 1.4 dB compared with binary PAM. Although both the theoretical analysis and simulations are based on time-hopping multiple access, this modulation scheme can also be applied to other accessing techniques of UWB communication systems.
基金supported by the National High-tech R&D Program (863 Program) 2013AA041003the Natural Science Foundation of China under Grants 51165033the Science and Technology Department of Jiangxi Province of China under grant 20151BBE50046,20142BBE50035 and 20151BAB207052
文摘Based on the proposed partly equidifferent mapping and its specific Differential Amplitude and Pulse Position Modulation(DAPPM) demodulation, a modified FSO scheme for turbulent channel is designed and analyzed. The novel Low Density Parity Check(LDPC) coded 4×4 and 4×8 DAPPM Free-Space Optical communication(FSO) system is constructed. The Monte Carlo simulation results show approximately 2d B transmit power reduction against classical LDPC-DAPPM at the identical Bit-Error-Rate in strong turbulent channel. The proposed partly equidifferent mapping is compatible with other modulations, so it enables widespread adoption in other coded FSO systems.
文摘Over the last decades, the usage of PAM （pulse amplitude modulated） fluorometers for assessment of chlorophyll-a fluorescence variations became widely applied on marine macroalgae physiology and ecophysiology researches. Due to the increased use of these methods, a large number of studies, mainly relating to macroalgae ecology and physiology were worldwide reported. In this context, it was also created a mismatch of concepts about fluorescence of the chlorophyll-a and its application. Under this background, this study compile and summarize the state of the art knowledge regarding to the chlorophyll fluorescence, contextualizing the use of the PAM method with the main factors regulating photosynthesis （light, temperature, salinity nitrogen and phosphorus） in marine macroalgae. Moreover, this study also references the most used terms and shows some examples found in literature about the applicability of fluorescence parameters. The herein findings and the discussed examples, helps to emphasize the importance of fluorescence usage, that highlights the understanding of photosynthetic responses in macroalgal physiology and ecology.
文摘Converters with pulse width modulation are used for connections between the direct current （DC） and alternating current （AC） networks, e.g., in uninterrupted power supply systems, AC electromotor drives, for powering induction furnaces, in audio technique. Spectrum of signals sampled by pulse amplitude modulation and output voltage spectrum of the converter with pulse width modulation have similar properties. Spectrum of signals sampled by pulse amplitude modulation contains a harmonic of frequency equal to the frequency of the modulating signal and the harmonics of frequencies equal to the sum of frequency of the modulating signal and multiples of the sampling frequency. The output voltage spectrum of the converter with bipolar pulse width modulation contains harmonic of frequency equal to the frequency of the modulating signal and harmonics of frequencies equal to sum of the frequency of the modulating signal and multiples of the frequency of the carrier signal. It also contains harmonics of frequencies equal to the sum of the multiples of the frequency of the modulating signal and the multiples of the carrier signal. The comparison analysis was carried out for the harmonics of the output voltage of the converter with bipolar pulse width modulation in time domain. The dependency of the amplitudes and frequency spectrum on the wave forms of the carrier signal and modulating signal was shown. Similarity of the output voltage spectrum of the converter and signal spectrum sampled by the pulse width modulation was also shown. Key words： Output voltage converter with bipolar pulse width modulation, spectral analysis, Fourier series, carrier signal, reference signal.
基金supported by the National Natural Science Foundation of China(No.61475015)
文摘A cost-effective ultra-dense wavelength-division-multiplexed passive optical network(UD-WDM PON) with speed of 12.5 Gbit/s and channel spacing of 12.5 GHz is proposed and demonstrated. The distributed feedback(DFB) lasers modulated in 4-level pulse amplitude modulation(4-PAM) format are used for downstream links, and the reflective semiconductor optical amplifiers(RSOAs) together with an optical frequency comb modulated in quadrature phase shift keying(QPSK) format are used for upstream links. We can achieve the error-free transmission of the upstream signals with speed of 12.5 Gbit/s even after 20 km single-mode fiber(SMF). The power penalty obtained by using the frequency comb generator instead of a tunable laser is around 0.5 d B. By using 11 DFB lasers and a set of intensity and phase modulators, it is possible to provide the seed light for 297 optical network units(ONUs) within the C-band.
