Objective: To observe the dynamic changes of fre e iron contents and its relationship to the changes of lipid peroxidation after experimental spinal cord injury (SCI). Methods: Sprague Dawley rats were randomly divide...Objective: To observe the dynamic changes of fre e iron contents and its relationship to the changes of lipid peroxidation after experimental spinal cord injury (SCI). Methods: Sprague Dawley rats were randomly divided into three g roups: Group A (n=6) received no operation; Group B (n=48) received only laminec tomy (sham); and Group C (n=48) received both laminectomy and traumatic injury ( SCI model). The SCI animal models were made by using an modified Allens weight -drop device (50 g.cm) on T_ 12 . Rats were sacrificed at 0.5 ,1,3,6,12,24 hours after injury. The levels of free iron involved in spinal cord segm ents at different time points were measured by bleomycin assay. The malondialdeh yde (MDA) was also measured by the thiobarbituric acid (TBA). Results: After SCI in Group C,the level of free iron showed a significant increase at 0.5 hour compared to Groups B and A,restored to th e control level at 6 h; the level of MDA was increased at 0.5 hour,peaked a t 3 hours,returned to the control level at 12 hours; the concentrations of free iron and lipid peroxidation in injured rats were significantly and positively c orrelated at 0.5 -3 hours. Conclusions: After SCI the levels of free iron are increased qu ickly and might be a major contributor to lipid peroxidation in injured spinal c ord.展开更多
Objective: To observe pressure changes in the spinal canal of the vertebrarium subjected to impact. From the point of view of impact, pressure changes and spinal cord injuries, the relationship between the type of spi...Objective: To observe pressure changes in the spinal canal of the vertebrarium subjected to impact. From the point of view of impact, pressure changes and spinal cord injuries, the relationship between the type of spinal fracture and the severity of spinal cord injuries were analyzed and some experimental data were provided for early evaluation of severity of spinal cord injuries. Methods: An experimental model of spinal burst fracture was made with Type BIM I bio impact machine and techniques of high velocity vertical loading in static pattern and stress shielding were adopted. Vertebral sections T10 L4 taken from fresh cadavers were impacted and pressure changes in the spinal canal were observed. The types and severity of spinal fracture were studied with gross and radiography examination. Results: Great positive pressure wave (wave A) in the spinal canal of the 4 vertebral specimens with burst fracture was recorded. The peak value of pressure was correlated with the severity of posterior column injuries. Generally, the peak value of pressure was low in the samples with posterior column injuries, but high in the samples without injuries. The predominant features of fractures were burst fractures of vertebral body and severe destruction of the skeletal and fiber structure of the spinal canal. Positive and negative pressure waves (wave B) were recorded in 2 vertebral samples in which no significant abnormal changes were found by radiography examination, however, a little liquid effusion in the vertebral body was found by gross examination. Conclusions: The type of pressure wave in the spinal canal is related to the deformation or the destruction of the spinal canal structure. The peak value of the pressure is non linearly related to the obstruction in the spinal canal, but related to posterior column injuries.展开更多
Since the late 20th century owing to the improvement of spinal surgery techniques, the diagnosis and treatment of thoracolumbar fracture have been perfected more and more. Although the advent of modern spinal surgery ...Since the late 20th century owing to the improvement of spinal surgery techniques, the diagnosis and treatment of thoracolumbar fracture have been perfected more and more. Although the advent of modern spinal surgery in China was late, we have gained some advanced achievements owing to various international communications benefited from the open policy. Therefore, it is essential to evaluate the current status and perspective of diagnosis and treatment of thoracolumbar fracture. There are several issues we would like to discuss here.展开更多
文摘Objective: To observe the dynamic changes of fre e iron contents and its relationship to the changes of lipid peroxidation after experimental spinal cord injury (SCI). Methods: Sprague Dawley rats were randomly divided into three g roups: Group A (n=6) received no operation; Group B (n=48) received only laminec tomy (sham); and Group C (n=48) received both laminectomy and traumatic injury ( SCI model). The SCI animal models were made by using an modified Allens weight -drop device (50 g.cm) on T_ 12 . Rats were sacrificed at 0.5 ,1,3,6,12,24 hours after injury. The levels of free iron involved in spinal cord segm ents at different time points were measured by bleomycin assay. The malondialdeh yde (MDA) was also measured by the thiobarbituric acid (TBA). Results: After SCI in Group C,the level of free iron showed a significant increase at 0.5 hour compared to Groups B and A,restored to th e control level at 6 h; the level of MDA was increased at 0.5 hour,peaked a t 3 hours,returned to the control level at 12 hours; the concentrations of free iron and lipid peroxidation in injured rats were significantly and positively c orrelated at 0.5 -3 hours. Conclusions: After SCI the levels of free iron are increased qu ickly and might be a major contributor to lipid peroxidation in injured spinal c ord.
文摘Objective: To observe pressure changes in the spinal canal of the vertebrarium subjected to impact. From the point of view of impact, pressure changes and spinal cord injuries, the relationship between the type of spinal fracture and the severity of spinal cord injuries were analyzed and some experimental data were provided for early evaluation of severity of spinal cord injuries. Methods: An experimental model of spinal burst fracture was made with Type BIM I bio impact machine and techniques of high velocity vertical loading in static pattern and stress shielding were adopted. Vertebral sections T10 L4 taken from fresh cadavers were impacted and pressure changes in the spinal canal were observed. The types and severity of spinal fracture were studied with gross and radiography examination. Results: Great positive pressure wave (wave A) in the spinal canal of the 4 vertebral specimens with burst fracture was recorded. The peak value of pressure was correlated with the severity of posterior column injuries. Generally, the peak value of pressure was low in the samples with posterior column injuries, but high in the samples without injuries. The predominant features of fractures were burst fractures of vertebral body and severe destruction of the skeletal and fiber structure of the spinal canal. Positive and negative pressure waves (wave B) were recorded in 2 vertebral samples in which no significant abnormal changes were found by radiography examination, however, a little liquid effusion in the vertebral body was found by gross examination. Conclusions: The type of pressure wave in the spinal canal is related to the deformation or the destruction of the spinal canal structure. The peak value of the pressure is non linearly related to the obstruction in the spinal canal, but related to posterior column injuries.
文摘Since the late 20th century owing to the improvement of spinal surgery techniques, the diagnosis and treatment of thoracolumbar fracture have been perfected more and more. Although the advent of modern spinal surgery in China was late, we have gained some advanced achievements owing to various international communications benefited from the open policy. Therefore, it is essential to evaluate the current status and perspective of diagnosis and treatment of thoracolumbar fracture. There are several issues we would like to discuss here.
