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脑源性神经营养因子修饰人羊膜间充质干细胞移植改善阿尔茨海默病大鼠认知功能 被引量:4
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作者 王玉香 崔传举 +1 位作者 李彦玲 李艾帆 《中国组织工程研究》 CAS 北大核心 2022年第13期2045-2049,共5页
背景:研究发现脑源性神经营养因子(BDNF)修饰的人间充质干细胞可用于治疗脊髓损伤和创伤性脑损伤等,但对阿尔茨海默病是否有疗效,既往鲜有报道。目的:观察脑源性神经营养因子修饰人羊膜间充质干细胞(human amniotic mesenchymal stem ce... 背景:研究发现脑源性神经营养因子(BDNF)修饰的人间充质干细胞可用于治疗脊髓损伤和创伤性脑损伤等,但对阿尔茨海默病是否有疗效,既往鲜有报道。目的:观察脑源性神经营养因子修饰人羊膜间充质干细胞(human amniotic mesenchymal stem cells,hAMSCs)对阿尔茨海默病大鼠学习记忆能力和海马胆碱乙酰转移酶、基底前脑神经生长因子表达的影响。方法:40只健康雄性成年SD大鼠随机分为对照组、模型组、hAMSCs组、BDNF-hAMSCs组,每组10只。采用双侧海马注射β-淀粉样蛋白25-35构建阿尔茨海默病模型,第14天后侧脑室注射10μL的hAMSCs或10μL的脑源性神经营养因子转染hAMSCs。移植后2周,采用Morris水迷宫测试评价大鼠学习记忆能力,免疫组化法检测海马胆碱乙酰转移酶的表达,RT-PCR检测基底前脑神经生长因子mRNA的表达。结果与结论:①Morris水迷宫测试第3,4,5天时,hAMSCs组和BDNF-hAMSCs组逃避潜伏期均低于模型组(P<0.05);测试第4,5天时,BDNF-hAMSCs组逃避潜伏期低于hAMSCs组(P<0.05);②模型组海马胆碱乙酰转移酶阳性神经元数、基底前脑神经生长因子表达量明显低于对照组(P<0.05);hAMSCs组和BDNF-hAMSCs组海马胆碱乙酰转移酶阳性神经元数、基底前脑神经生长因子表达量均高于模型组(P<0.05),并且BDNF-hAMSCs组高于hAMSCs组(P<0.05);③结果表明,用脑源性神经营养因子修饰hAMSCs可进一步提高hAMSCs治疗阿尔茨海默病的认知功能,并且可以明显提高海马胆碱乙酰转移酶和基底前脑神经生长因子的表达水平。 展开更多
关键词 干细胞 人羊膜间充质干细胞 脑源性神经营养因子 阿尔茨海默病 胆碱乙酰转移酶 脑神经生长因子 认知功能
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17-β雌二醇对血管性痴呆大鼠NGF表达的影响 被引量:1
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作者 姜晓蕊 卢宏 +1 位作者 冯建玉 滕军放 《实用诊断与治疗杂志》 2005年第2期106-108,F002,共4页
目的:研究17-β雌二醇对血管性痴呆大鼠脑组织神经生长因子表达的影响。方法:采用结扎双侧颈总动脉 方法制备慢性前脑缺血动物模型,应用Y迷宫,免疫组化及ELISA法等检测腹腔注射17-β雌二醇60 d后VD大鼠认知功 能以及脑组织中神经生长因... 目的:研究17-β雌二醇对血管性痴呆大鼠脑组织神经生长因子表达的影响。方法:采用结扎双侧颈总动脉 方法制备慢性前脑缺血动物模型,应用Y迷宫,免疫组化及ELISA法等检测腹腔注射17-β雌二醇60 d后VD大鼠认知功 能以及脑组织中神经生长因子含量变化。结果:与正常对照组对比,大鼠学习尝试次数,记忆测试10次的正确次数在造 模60 d后差异有显著性意义(P<0.05),腹腔注射17-β雌二醇60 d后,大鼠认知功显著改善;造模60 d后的大鼠,其脑内 神经生长因子免疫阳性细胞数较正常对照组显著增高,雌二醇组大鼠脑组织内神经生长因子免疫阳性细胞数较模型组 显著增高。神经生长因子在三组大鼠脑内含量的测定结果与之一致。结论:腹腔注射17-β雌二醇可显著改善VD大鼠的 认知功能,增加VD大鼠脑内神经生长因子含量。 展开更多
关键词 17-Β雌二醇 血管性痴呆 脑神经生长因子
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脑力苏胶囊对血管性痴呆小鼠脑组织NGF表达的影响
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作者 梅建伟 张英强 《现代临床医学》 2010年第3期174-175,共2页
目的:研究脑力苏胶囊对血管性痴呆(VD)模型小鼠脑组织神经生长因子(NGF)表达的影响,以探讨脑力苏胶囊治疗VD的部分作用机理。方法:采用结扎双侧颈总动脉方法制备血管性痴呆小鼠动物模型,用酶联免疫吸附法(ELISA法)测定给药后VD小鼠脑组... 目的:研究脑力苏胶囊对血管性痴呆(VD)模型小鼠脑组织神经生长因子(NGF)表达的影响,以探讨脑力苏胶囊治疗VD的部分作用机理。方法:采用结扎双侧颈总动脉方法制备血管性痴呆小鼠动物模型,用酶联免疫吸附法(ELISA法)测定给药后VD小鼠脑组织内神经生长因子(NGF)的含量变化。结果:给药15 d后,VD小鼠脑内神经生长因子(NGF)较模型对照组显著增高。结论:脑力苏胶囊可增加VD大鼠脑内神经生长因子(NGF)的含量,可能与改善VD小鼠的认知功能障碍作用相关。 展开更多
关键词 脑力苏胶囊 血管性痴呆小鼠 脑神经生长因子(NGF)
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桑芪首乌片对局灶性脑缺血再灌注大鼠BDNF及其受体TrkB表达的影响 被引量:1
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作者 顾力华 陈奇刚 +4 位作者 陆家龙 魏丹霞 杨仁华 陈鹏 司林阁 《广州中医药大学学报》 CAS 2020年第2期324-328,共5页
【目的】探讨桑芪首乌片对大鼠脑缺血再灌注损伤后脑源性神经营养因子(BDNF)及其酪氨酸激酶受体B(TrkB)表达的影响。【方法】将70只SD雄性大鼠随机分为假手术对照组,模型对照组,天麻钩藤颗粒对照组(剂量为2.0 g·kg^-1·d^-1),... 【目的】探讨桑芪首乌片对大鼠脑缺血再灌注损伤后脑源性神经营养因子(BDNF)及其酪氨酸激酶受体B(TrkB)表达的影响。【方法】将70只SD雄性大鼠随机分为假手术对照组,模型对照组,天麻钩藤颗粒对照组(剂量为2.0 g·kg^-1·d^-1),尼莫地平片对照组(剂量为7.7 mg·kg-1·d^-1),桑芪首乌片高、中、低剂量组(生药剂量分别为2.8、1.4、0.7 g·kg^-1·d^-1),每组10只。采用线栓法建立局灶性脑缺血再灌注大鼠模型。造模后24 h内开始第1次灌胃给药,连续6周。给药结束后,观察各组大鼠神经行为学评分,采用免疫组织化学染色法观察各组大鼠脑组织BDNF、TrkB的表达。【结果】与模型对照组比较,桑芪首乌片高、中剂量组大鼠给药3周后的神经行为评分明显降低(P<0.05),桑芪首乌片高、中、低剂量组BDNF、TrkB阳性细胞数量明显增加(P<0.05),均呈剂量依赖性。【结论】桑芪首乌片能通过上调脑缺血再灌注大鼠脑组织BDNF、TrkB的表达,从而发挥神经保护作用。 展开更多
关键词 桑芪首乌片 脑缺血再灌注 脑神经源性神经生长因子 酪氨酸激酶B 疾病模型 动物 大鼠
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Cognitive improvement following transvenous adipose-derived mesenchymal stem cell transplantation in a rat model of traumatic brain injury 被引量:7
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作者 Dongfei Li Chun Yang Rongmei Qu Huiying Yang Meichun Yu Hui Tao Jingxing Dai Lin Yuan 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第10期732-737,共6页
The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model... The effects of adipose-derived mesenchymal stem cell (ADMSC) transplantation for the repair of traumatic brain injury remain poorly understood. The present study observed neurological functional changes in a rat model of traumatic brain injury following ADMSC transplantation via the tail vein. Cell transplants were observed in injured cerebral cortex, and expression of brain-derived nerve growth factor was significantly increased in the injured hippocampus following transplantation. Results demonstrated that transvenous ADMSC transplants migrated to the injured cerebral cortex and significantly improved cognitive function. 展开更多
关键词 adipose-derived mesenchymal stem cells traumatic brain injury brain-derived nerve growth factor CORTEX stem cell transplantation neural regeneration
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Changes of Nerve Growth Factor in Amniotic Fluid and Correlation with Ventriculomegaly 被引量:1
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作者 Xiao-yan Xia Xing-hua Huang +1 位作者 Yi-xin Xia Wei-hua Zhang 《Chinese Medical Sciences Journal》 CAS CSCD 2011年第2期109-112,共4页
Objective To detect the change of nerve growth factor (NGF) level in human amniotic fluid during gestation, and to explore the relationship between this change and fetal ventriculo-megaly (VM). Methods The studied sub... Objective To detect the change of nerve growth factor (NGF) level in human amniotic fluid during gestation, and to explore the relationship between this change and fetal ventriculo-megaly (VM). Methods The studied subjects (collected from 2004 to 2007) were divided into four groups, including the second-trimester pregnancy group (n=113), third-trimester pregnancy group (n=110), fetal cerebral VM group (n=12), and healthy control group (n=12) which matched with the VM group in gestational weeks. The amniotic fluid specimens were obtained during amniocentesis or cesarean section. The NGF levels in amniotic fluid were detected with en-zyme-linked immunosorbent assay. Results A significantly negative correlation was found between gestational age and the NGF level in amniotic fluid (r= 0.6149, P<0.0001). The NGF level in patients with fetal VM was significantly lower than that in healthy controls (33.95±29.24 pg/mL vs. 64.73±16.21 pg/mL, P=0.024). Conclusion NGF levels in amniotic fluid may be a sensitive marker for fetal VM. 展开更多
关键词 nerve growth factor amniotic fluid fetal ventriculomegaly
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The expressions of brain-derived neurotrophic factor and nerve growth factor in purified rat choroid plexus epithelial cells in vitro 被引量:1
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作者 CHEN Bo MIAO Xingyu +4 位作者 SHI Wei PU Jingnan LIU Chongxiao GUO Zhenyu WANG Fangru 《Journal of Medical Colleges of PLA(China)》 CAS 2013年第5期257-267,共11页
Objective: To detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro. Methods: Primary and passage choroid plexu... Objective: To detect the expressions of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) in purified rat choroid plexus epithelial cells in vitro. Methods: Primary and passage choroid plexus epithelial cells were obtained from newborn, one-day Spragne-Dawley rats. The expressions of BDNF and NGF were measured by qRT-PCR and Western blottingting. The secretions of BDNF and NGF were detected by ELISA. Cell supematants of primary cells, purified cells and passage 1 cells were harvested. Results: The expression of BDNF in the purified cells was significantly lower than that in the primary cells (P〈0.05), and it in the primary cells and the purified cells was significantly higher than that in the passage 1 cells (P〈0.05). The expression of NGF was significantly higher in the purified cells than in the primary cells and the passage 1 cells (P〈0.05). It in the passage 1 cells was significantly higher than that in the primary cells (P〈0.05). Conclusion: The time of CPECs transplantation for central nervous system diseases should be selected based on their secretory function and features,which could lead to better and more effective treatment. 展开更多
关键词 Brain derived-neurotrophic factor Nerve growth factor Choroid plexus epithefial cells Primary culture Passage culture Serial subcultivation
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HCMV Infection Depress NGF Expression in Human Glioma Cells
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作者 Hai-tao WANG Bin WANG +5 位作者 Zhi-jun LIU Zhi-qiang BAI Ling LI Dong-meng QIAN Zhi-yong YAN Xu-xia SONG 《Virologica Sinica》 SCIE CAS CSCD 2009年第3期209-214,共6页
Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of... Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia ceils by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells. 展开更多
关键词 Human cytomegalovirus (HCMV) U251 cell Nerve growth factor (NGF)
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Upregulated gene expression of local brain-derived neurotrophic factor and nerve growth factor after intracisternal administration of marrow stromal cells in rats with traumatic brain injury 被引量:5
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作者 胡德志 周良辅 +2 位作者 朱剑虹 毛颖 吴雪海 《Chinese Journal of Traumatology》 CAS 2005年第1期23-26,共4页
Objective: To examine the effects of rat marrow stromal cells (rMSCs) on gene expression of local brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) after injection of rMSCs into Cistern Magnum of ... Objective: To examine the effects of rat marrow stromal cells (rMSCs) on gene expression of local brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) after injection of rMSCs into Cistern Magnum of adult rats subjected to traumatic brain injury(TBI).Methods: A modified Feeney’s TBI model was created in 48 adult rats. rMSCs were harvested from 3-month-old rats, and injected into Cistern Magnum in 24 rats subjected to TBI(Group cell transplantation). Saline was given through Cistern Magnum to another 24 rats subjected to TBI (Group saline control). Animals were sacrificed 1, 2 and 3 weeks after intervention, and special brain tissue blocks were dissected for total RNA extraction from each block. BDNF and NGF mRNA were reverse-transcribed into cDNA and further expanded by polymerase chain reaction (PCR). The expression of target genes was evaluated using semi-quantitative methods. Results: Group cell transplantation had higher BDNF and NGF gene expressions than Group saline control during a period of less than 3 weeks (P< 0.05).Conclusions: rMSCs transplantation via Cistern Magnum in rats subjected to traumatic brain injury can enhance expressions of local brain NGF and BDNF to a certain extent. 展开更多
关键词 Marrow stromal cells Brain injuries Reverse-transcription polymerase chain Brain-derived neurotrophic factor Nerve growth factor
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Research progress of the role and mechanism of extracellular signal-regulated protein kinase 5(ERK5) pathway in pathological pain
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作者 Li-na YU Li-hong SUN +1 位作者 Min WANG Min YAN 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2016年第10期733-741,共9页
Extracellular signal-regulated protein kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (MAPK1), is an important member of ERK family, which is a subfamily of the large MAPK family. ERK5 is ex... Extracellular signal-regulated protein kinase 5 (ERK5), also known as big mitogen-activated protein kinase 1 (MAPK1), is an important member of ERK family, which is a subfamily of the large MAPK family. ERK5 is expressed in many tissues, including the dorsal root ganglion (DRG) neurons and the spinal cord. In this review, we focus on elaborating ERK5-associated pathway in pathological pain, in which the ERK5/CREB (cyclic adenosine monophos- phate (cAMP)-response element-binding protein) pathway plays a crucial role in the transduction of pain signal and contributes to pain hypersensitivity. ERK5 activation in the spinal dorsal horn occurs mainly in microglia. The activation of ERK5 can be mediated by N-methyI-D-aspartate (NMDA) receptors. We also elaborate the relationship between ERK5 activation and nerve growth factor-tyrosine kinase A (NGF-TrkA), and the connection between ERK5 activation and brain-derived neurotrophic factor (BDNF) in pathological pain in detail. 展开更多
关键词 Extracellular signal-regulated protein kinase 5 (ERK5) Pain Cyclic adenosine monophosphate (cAMP)-response element-binding protein (CREB) N-methyl-[^-aspartate (NMDA) Nerve growth factor (NGF) Brain-derived neurotrophic factor (BDNF)
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Effects of electroacupuncture on angiogenesis and cortical VEGF and BDNF expression in rats with focal cerebral ischemia
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作者 LI Mengxing WANG Yu +3 位作者 GAO Yunyun YAO Xiaowen LAN Wei TANG Wei 《Journal of Acupuncture and Tuina Science》 CSCD 2022年第2期91-103,共13页
Objective To observe the effect of electroacupuncture(EA)of“concurrent treatment of the brain and heart”on angiogenesis and cortical vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF... Objective To observe the effect of electroacupuncture(EA)of“concurrent treatment of the brain and heart”on angiogenesis and cortical vascular endothelial growth factor(VEGF)and brain-derived neurotrophic factor(BDNF)in rats with focal cerebral ischemia,and to explore the mechanism of EA in cerebral ischemia treatment.Methods A total of 108 Sprague-Dawley rats,27 rats were randomly selected as the sham-operation group,and the rest rats received the right middle cerebral artery occlusion operation for model preparation firstly,and then were divided into a model group,a traditional acupoint group,and a concurrent treatment of the brain and heart group,with 27 rats in each group.In the sham-operation group,only the carotid artery was isolated.EA at Shuigou(CV26),Quchi(LI11),Hegu(LI4),and Zusanli(ST36)in the traditional acupoint group,and EA at Fengfu(GV16),Baihui(GV20),Xinshu(BL15),and Neiguan(PC6)in the concurrent treatment of the brain and heart group were performed 4 h after the operation,once a day,for 14 consecutive days.Rats in the sham-operation group and the model group were identically fixed without any treatment.Before and after treatment,the modified neurological severity score(mNSS),regional cerebral blood flow(rCBF),and CD34 positive expression by immunohistochemistry were measured.The positive protein expression levels of VEGF and BDNF were detected by immunofluorescence,and the mRNA expression levels of VEGF and BDNF were detected by quantitative reverse transcription-polymerase chain reaction(qRT-PCR).Results Compared with the sham-operation group,the mNSS,rCBF,and ischemic side cortical micro-vessel density(MVD)decreased,and the protein and mRNA expression levels of VEGF and BDNF increased in the model group(P<0.01).Compared with the model group,the mNSS of the two EA groups decreased,and the mNSS of the concurrent treatment of the brain and heart group was lower than that of the traditional acupoint group on the 14th day(P<0.05).Compared with the model group,the rCBF in the two EA groups increased,and the rCBF reached the highest on the 14th day(P<0.05 or P<0.01),and the rCBF in the concurrent treatment of the brain and heart group was higher than that in the traditional acupoint group(P<0.05);the MVD of the two EA groups was higher than that of the model group,and the MVD of the concurrent treatment of the brain and heart group was higher than that of the traditional acupoint group on the 7th and 14th days(P<0.05 or P<0.01).Compared with the model group,the protein and mRNA expression levels of VEGF and BDNF in the two EA groups increased(P<0.01).The VEGF expression level was the highest on the 7th day in the concurrent treatment of the brain and heart group(P<0.05),and the BDNF expression level was higher on the 7th and 14th days than on the 3rd day(P<0.05).The mRNA expression levels of VEGF and BDNF in both EA groups reached the highest on the 7th day(P<0.05 or P<0.01).Conclusion EA therapy can up-regulate the VEGF and BDNF expression levels and increase the rCBF in the cortex of rats with focal cerebral ischemia,which may be one mechanism of EA in the cerebral ischemia treatment.The therapeutic effect is accumulated with the effective time,and the concurrent treatment of the brain and heart group is superior to the traditional acupoint group in promoting angiogenesis. 展开更多
关键词 Acupuncture Therapy ELECTROACUPUNCTURE Brain Ischemia Ischemic Stroke Cerebrovascular Circulation Vascular Endothelial Growth Factors Brain-derived Neurotrophic Factor RATS
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