益智仁提取物原儿茶酸对大鼠局灶性脑缺血再灌注损伤的保护作用

目的应用SD大鼠局灶性脑缺血模型,研究原儿茶酸对脑缺血性再灌注损伤的保护作用。方法健康雄性成年SD大鼠36只,随机分为三组(n=12):假手术组(S组)、脑缺血再灌注组(IR组)和原儿茶酸处理组(PCA组)。采用大脑中动脉阻断法制备大鼠局灶性脑缺血再灌注模型。大鼠腹腔注射PCA,25mg/kg、1次/d、连续7d。PCA组于预处理结束后24h,制备局灶性脑缺血再灌注模型。再灌注24h后,行神经功能评分,随后处死大鼠,取脑组织,测定脑梗死体积百分比,采用PT-PCR法及Western blot测定缺血半暗带NF-κB-p65及Notch1的表达。结果与IR组比较,PCA组脑梗死体积百分比降低,神经功能评分升高,脑组织缺血区半暗带NF-κB-p65及Notch1表达下调(P<0.05)。结论 PCA预处理可能通过抑制脑组织NF-κB-p65及Notch1的表达、减轻了大鼠局灶性脑缺血再灌注的损伤。

对缺血性再灌注脑损伤大鼠采用盐酸托哌酮联合骨髓间充质干细胞移植进行治疗的效果探讨

目的 :探讨对缺血性再灌注脑损伤大鼠采用盐酸托哌酮联合骨髓间充质干细胞移植进行治疗的效果。方法 :将中南大学湘雅医学院实验动物中心提供的48只健康雄性大鼠作为研究对象。将这些研究对象随机分为模型组、干细胞移植组、盐酸托哌酮组及联合治疗组,每组各有12只大鼠。为四组大鼠建立缺血性再灌注脑损伤模型。建模后,对模型组大鼠不使用任何方法进行治疗。对干细胞移植组大鼠采用骨髓间充质干细胞移植进行治疗。对盐酸托哌酮组大鼠采用盐酸托哌酮进行治疗。对联合治疗组大鼠采用盐酸托哌酮联合骨髓间充质干细胞移植进行治疗。然后,比较四组大鼠在接受治疗后的第3 d及第7 d其神经功能缺损的评分、CD34阳性细胞数及脑梗死的体积。结果 :接受治疗后的第3 d及第7 d,与模型组大鼠相比,干细胞移植组大鼠、盐酸托哌酮组大鼠及联合治疗组大鼠神经功能缺损的评分均较低,其CD34阳性细胞数较多,其脑梗死的体积较小(P<0.05)。与接受治疗后的第3d相比,在治疗后的第7d干细胞移植组大鼠、盐酸托哌酮组大鼠及联合治疗组大鼠神经功能缺损的评分均较低,其CD34阳性细胞数较多,其脑梗死的体积较小(P <0.05)。在接受治疗后的第3d及第7d,模型组大鼠神经功能缺损的评分、CD34阳性细胞数、脑梗死的体积相比,差异均无统计学意义(P>0.05)。结论 :对缺血性再灌注脑损伤大鼠采用盐酸托哌酮联合骨髓间充质干细胞移植进行治疗的效果显著,可促进其神经功能的恢复,减小其脑梗死的体积。

EFFECTS OF ELECTROACUPUNCTURE ON EXPRESSION OF INTERCELLULAR ADHESION MOLECULE-1 IN THE RAT OF LOCAL CEREBRAL ISCHEMIA-REPERFUSION

Objective To investigate effects of electroacupuncture （EA） on expression of intercellular adhesion molecule-1 （ICAM-1） in the rat of local cerebral ischemia-reperfusion. Methods Eighty SD rats were randomly divided into a normal control group, a sham operation group, a model group and an EA treatment group, 20 rats in each group. The thread-obstruction method was used for preparation of ischemia-reperfusion model. Zea-Longa rating criteria were used for evaluation of nervous function disorder; Immunohistochemical SABC method was used for detection of ICAM-1 expression in the microvascular endothelial cell of the ischemic brain region, and ELISA method for the soluble ICAM-1 （slCAM-1） content in peripheral blood. Re. suits After cerebral ischemia-reperfusion, both ICAM-1 expression level in the microvascular endethelium cell of the ischemic brain region and slCAM-1 content in the peripheral blood significantly increased in the model group as compared with the normal group and the sham operation group （P〈0.01）; After EA treatment, the ICAM-1 expression level in the microvascular endothelial cell of the ischemic brain region and slCAM-1 content in the peripheral blood were significantly down-regulated in the EA treatment group as com- pared with the model group （P〈 0.05）. Conclusion After cerebral ischemia-reperfusion, the microvascular endothelial cell of the ischemic brain region releases ICAM-1, which induces inflammatory injury of cerebral tissues; EA treatment can decease the expression of ICAM-1, so as to prevent the brain from the injury.

Effects of eye-acupuncture on the expression of brainderived neurotrophic factor in the brain of rats with cerebral ischemia reperfusion

Objective To observe the effects of eye-acupuncture therapy and bodyacupuncture therapy on the expression of brain-deprived neurotrophic factor （BDNF） in rats with cerebral ischemia reperfusion injury （CIRI）. Methods According to random number table, 48 SD rats were randomly divided into 6 groups, including normal control group （group A）, sham operation group （group B）, model group （group C）, eye-acupuncture group （group D）, non-acupoint of eye-acupuncture group （group E） and body-acupuncture group （group F）, eight rats in each group. Artery infarction reperfusion model were prepared by using suture-occluded method. Liver region, upper energizer area, lower energizer area and kidney region were selected in the group D. Acupuncture was carried out at the point located at 3 mm from the acupoint areas in the group E. Qūchí （曲池 LI 11）, Zúsānl （足三里 ST 36） and other acupoints were selected in the group F. Zea Longa scoring method was utilized for scoring the neural functions of rats; real-time PCR was carried out to examine the expression level of BDNF mRNA in the brain 72 h after ischemia reperfusion; western blot was carried out to examine the expression level of BDNF protein in the brain 72 h after ischemia reperfusion. Results The symptoms of neurologic impairments in the rats of the group D were alleviated in comparison to those in the group C （P0.01）, and the difference between the group D and the group F was not statistically significant （P0.05）; Compared with the group C, the mRNA and protein expression levels of BDNF in the brain of rats in the group D and the group F both increased （P0.01）, but the difference between the group D and the group F was not statistically significant （P0.05）. Conclusion The functions of eye-acupuncture and body-acupuncture in improving cerebral ischemia reperfusion injury are similar, and the functional mechanisms for the two different therapies may be related to the up-regulation of BDNF expression in brain and thus promote the repairing of brain tissues.