Porcine reproductive and respiratory syndrome virus(PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation(APA) is a mechanism that diversifies gene expression, which is impo...Porcine reproductive and respiratory syndrome virus(PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation(APA) is a mechanism that diversifies gene expression, which is important for tumorigenesis, development, and cell differentiation. However, it is unclear whether APA plays a role in the course of PRRSV infection. To address this issue, in this study we carried out a whole-genome transcriptome analysis of PRRSVinfected Marc-145 African green monkey kidney cells and identified 185 APA switching genes and 393 differentially expressed genes(DEGs). Most of these genes were involved in cellular process, metabolism, and biological regulation, and there was some overlap between the two gene sets. DEGs were found to be more directly involved in the antiviral response than APA genes. These findings provide insight into the dynamics of host gene regulation during PRRSV infection and a basis for elucidating the pathogenesis of PRRSV.展开更多
基金传染病预防控制国家重点实验室基金(No.2008SKLID1022011SKLID211)+2 种基金the SKLID development Grant"十一五"国家科技重大专项(No.2009ZX10004-101)China Mega-Project for Infectious Disease~~
基金supported by the Natural Science Foundation of Guangdong Province(2014A030312011)Guangzhou Science and Technology Plan(201804020039)
文摘Porcine reproductive and respiratory syndrome virus(PRRSV) causes substantial economic losses to the global pig industry. Alternative polyadenylation(APA) is a mechanism that diversifies gene expression, which is important for tumorigenesis, development, and cell differentiation. However, it is unclear whether APA plays a role in the course of PRRSV infection. To address this issue, in this study we carried out a whole-genome transcriptome analysis of PRRSVinfected Marc-145 African green monkey kidney cells and identified 185 APA switching genes and 393 differentially expressed genes(DEGs). Most of these genes were involved in cellular process, metabolism, and biological regulation, and there was some overlap between the two gene sets. DEGs were found to be more directly involved in the antiviral response than APA genes. These findings provide insight into the dynamics of host gene regulation during PRRSV infection and a basis for elucidating the pathogenesis of PRRSV.
