燃料油和原油是海洋环境中石油污染的主要类型,其对海洋生物的毒性作用是评价海洋环境质量的基础,为了解两种石油污染在同一环境条件下对同一海洋生物的毒性差异,研究了0#柴油和原油水溶性成分(Water accommodated fraction,WAF)对黑鲷(...燃料油和原油是海洋环境中石油污染的主要类型,其对海洋生物的毒性作用是评价海洋环境质量的基础,为了解两种石油污染在同一环境条件下对同一海洋生物的毒性差异,研究了0#柴油和原油水溶性成分(Water accommodated fraction,WAF)对黑鲷(Sparus macrocephlus)肝脏7-乙氧基异吩噁唑酮-脱乙基酶(EROD)活性及细胞色素P4501A1(CYP1A1)m RNA表达量的影响.结果显示,在富集阶段(15 d),第1天0#柴油和原油WAF 0.06 mg/L和0.03 mg/L实验组肝脏的EROD活性、CYP1A1 m RNA表达量均显著增加(P<0.05),随后0#柴油和原油WAF两个浓度实验组肝脏的EROD活性均继续上升,分别在第5天、第10天达到峰值,随后下降;0#柴油WAF两个浓度实验组肝脏的CYP1A1 m RNA表达量在第1天后即开始下降,原油WAF两个浓度实验组肝脏的CYP1A1 m RNA表达量在第5天达到峰值后开始下降.在释放阶段(10 d)结束后,除了原油WAF 0.03 mg/L实验组外,0#柴油WAF的两个浓度实验组和原油WAF 0.06 mg/L实验组肝脏的EROD活性、CYP1A1 m RNA表达量均仍然显著高于对照组水平(P<0.05).本研究表明,两种石油污染物WAF对黑鲷肝脏的EROD活性、CYP1A1 m RNA诱导存在差异,0#柴油WAF实验组EROD活性、CYP1A1 m RNA表达量均高于原油WAF实验组,且0#柴油WAF实验组EROD活性、CYP1A1 m RNA表达量下降时间早于原油实验组,原油实验条件下黑鲷肝脏的EROD活性、CYP1A1 m RNA表达量的恢复能力高于0#柴油.展开更多
建立了全血中脱乙基扎来普隆液相色谱-串联质谱检验法。对p H、淋洗液、缓冲液等条件进行优化,实验选用HLB柱,p H 9硼酸盐缓冲溶液,氨水-甲醇水为淋洗液,乙腈为洗脱液,选用ZORBAX Eclipse Plus C18色谱柱,以A相0.1%甲酸和B相乙腈作为流...建立了全血中脱乙基扎来普隆液相色谱-串联质谱检验法。对p H、淋洗液、缓冲液等条件进行优化,实验选用HLB柱,p H 9硼酸盐缓冲溶液,氨水-甲醇水为淋洗液,乙腈为洗脱液,选用ZORBAX Eclipse Plus C18色谱柱,以A相0.1%甲酸和B相乙腈作为流动相,进行梯度洗脱。采用液相色谱-串联质谱仪的电喷雾电离,正离子模式扫描,MRM模式检测脱乙基扎来普隆。在最优条件下,全血中脱乙基扎来普隆质量浓度在0.1～100 ng/m L范围内有良好线性关系,保留时间为1.95 min。回归方程为y=9971.2ρ-1 813.8,检出限0.1 ng/m L。回收率90%以上,日内与日间精密度均小于10%。方法适用于全血中的脱乙基扎来普隆检测。展开更多
The prepared TiO2 suspension by sol-gel and hydrothermal method is spread on conducting glass substrates to prepare the photoelectrodes.The degradation of RhB at different bias under visible irradiation is researched....The prepared TiO2 suspension by sol-gel and hydrothermal method is spread on conducting glass substrates to prepare the photoelectrodes.The degradation of RhB at different bias under visible irradiation is researched.By the examination of UV-visible spectroscopy、degraded intermediates and removal yield of total organic carbon,the effect of bias on the photoelectrocatalytic degradation of RhB is investigated.It is found that N-dealkylation process predominates at positive bias(+0.6V vs SCE).Five N-de-ethylated intermediates are detected by HPLC.The removal yield of TOC is low,though dye RhB also discolors.But a rather facile cleavage of the whole conjugated chromophore structure of RhB at negative bias of-0.6 V vs SCE leads to the high mineralization yield.展开更多
文摘燃料油和原油是海洋环境中石油污染的主要类型,其对海洋生物的毒性作用是评价海洋环境质量的基础,为了解两种石油污染在同一环境条件下对同一海洋生物的毒性差异,研究了0#柴油和原油水溶性成分(Water accommodated fraction,WAF)对黑鲷(Sparus macrocephlus)肝脏7-乙氧基异吩噁唑酮-脱乙基酶(EROD)活性及细胞色素P4501A1(CYP1A1)m RNA表达量的影响.结果显示,在富集阶段(15 d),第1天0#柴油和原油WAF 0.06 mg/L和0.03 mg/L实验组肝脏的EROD活性、CYP1A1 m RNA表达量均显著增加(P<0.05),随后0#柴油和原油WAF两个浓度实验组肝脏的EROD活性均继续上升,分别在第5天、第10天达到峰值,随后下降;0#柴油WAF两个浓度实验组肝脏的CYP1A1 m RNA表达量在第1天后即开始下降,原油WAF两个浓度实验组肝脏的CYP1A1 m RNA表达量在第5天达到峰值后开始下降.在释放阶段(10 d)结束后,除了原油WAF 0.03 mg/L实验组外,0#柴油WAF的两个浓度实验组和原油WAF 0.06 mg/L实验组肝脏的EROD活性、CYP1A1 m RNA表达量均仍然显著高于对照组水平(P<0.05).本研究表明,两种石油污染物WAF对黑鲷肝脏的EROD活性、CYP1A1 m RNA诱导存在差异,0#柴油WAF实验组EROD活性、CYP1A1 m RNA表达量均高于原油WAF实验组,且0#柴油WAF实验组EROD活性、CYP1A1 m RNA表达量下降时间早于原油实验组,原油实验条件下黑鲷肝脏的EROD活性、CYP1A1 m RNA表达量的恢复能力高于0#柴油.
文摘The prepared TiO2 suspension by sol-gel and hydrothermal method is spread on conducting glass substrates to prepare the photoelectrodes.The degradation of RhB at different bias under visible irradiation is researched.By the examination of UV-visible spectroscopy、degraded intermediates and removal yield of total organic carbon,the effect of bias on the photoelectrocatalytic degradation of RhB is investigated.It is found that N-dealkylation process predominates at positive bias(+0.6V vs SCE).Five N-de-ethylated intermediates are detected by HPLC.The removal yield of TOC is low,though dye RhB also discolors.But a rather facile cleavage of the whole conjugated chromophore structure of RhB at negative bias of-0.6 V vs SCE leads to the high mineralization yield.
