To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods ： Deproteinized bone （ 10 mm） and leaching liquor were made...To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods ： Deproteinized bone （ 10 mm） and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test. Results ： No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramusclar implantation and cytotoxity were observed. Conclusions： The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering.展开更多
Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone m...Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone marrow stromal cells(BMSC)were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope,scanning electron microscope,MTT assay,flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth,adhesion in the material, cell cycle and cell viability. Results:The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation,differentiation and secretion function of BMSCs. Conclusions:The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.展开更多
文摘To evaluate the biological safety of manufactured heterologous deproteinized bone and to provide an experimental basis for clinical applications. Methods ： Deproteinized bone （ 10 mm） and leaching liquor were made from pig ribs with a series of physical and chemical methods, then were evaluated through acute and subacute toxicity test, hemolysis test, pyrogen test, intracutaneous test, intramuscular implantation test and cytotoxity test. Results ： No obvious toxicity, hemolysis, pyrogenic characteristics, skin irritation, inflammatory reaction after intramusclar implantation and cytotoxity were observed. Conclusions： The heterologous deproteinized bone has good biological safety and meets all the demands of scaffold material for tissue engineering.
文摘Objective:To study cellular compatibility of improved scaffold material with deproteinized heterogeneous bone and provide experimental basis on choosing the scaffold material in bone tissue engineering. Methods:Bone marrow stromal cells(BMSC)were co-cultured with heterogeneous deproteinized bone in vitro. The contrast phase microscope,scanning electron microscope,MTT assay,flow cytometry were performed and the BGP content and ALP activities were detected in order to observe the cell growth,adhesion in the material, cell cycle and cell viability. Results:The scaffold material of deproteinized heterogeneous bone had no inhibitory effect on cellular proliferation,differentiation and secretion function of BMSCs. Conclusions:The established heterogeneous deproteinized bone has good biocompatibility with BMSCs and is a potentially ideal scaffold material for bone tissue engineering.
