从纺织厂的工业废水排污口取样,分离筛选出了一株嗜热的高效偶氮染料脱色菌PDR2。通过形态学、生理生化特征以及16S r DNA基因序列分析等方法对该菌株进行鉴定,发现该菌为厌氧芽孢杆菌,与Anoxybacillus sp(GQ471935)同源性最高。此外,...从纺织厂的工业废水排污口取样,分离筛选出了一株嗜热的高效偶氮染料脱色菌PDR2。通过形态学、生理生化特征以及16S r DNA基因序列分析等方法对该菌株进行鉴定,发现该菌为厌氧芽孢杆菌,与Anoxybacillus sp(GQ471935)同源性最高。此外,分别考察了菌株PDR2在不同碳源、氮源、p H以及培养温度等条件下对偶氮染料直接黑38脱色性能的影响,在此基础上进一步研究了菌株PDR2对不同浓度直接黑38染料的耐受性。结果表明,菌株PDR2在55℃、p H 6.0且以葡萄糖为碳源,牛肉膏和蛋白胨为混合氮源时其脱色效果最佳,且菌株对偶氮染料直接黑38有着较强的耐受能力,在染料浓度低于0.4 g·L^(-1)时其脱色效果良好。在最佳条件下静置培养24 h时,菌株PDR2对0.1 g·L^(-1)直接黑38染料的脱色率高达92.34%,展出了良好的应用潜力。展开更多
This paper describes the effects of temperature on the complex intermediate processes from the precursor to the fully-crystallized anatase TiO2 nanoparticles in hydrothermal synthesis. The anatase TiO2 nanoparticles w...This paper describes the effects of temperature on the complex intermediate processes from the precursor to the fully-crystallized anatase TiO2 nanoparticles in hydrothermal synthesis. The anatase TiO2 nanoparticles were synthesized in a wide temperature range below 230°C. The composition, morphology, and methylene blue (MB) decoloration characteristics of the obtained products were investigated by X-ray diffraction, Fourier transform infrared spectroscope, X-ray photoelectron spectroscope, and scanning and transmission electron microscope. The dehydrating polycondensation of Ti(IV)-hydrates and the decomposition of (NH4)2Ti3O7 intermediates with the temperature increase lead to the direct formation of anatase TiO2 nanoparticles under the hydrothermal environments. The strong MB decoloration of the hydrothermal products obtained at the low (≤130°C) and high (≥180°C) temperatures are attributed to the adsorption of Ti(IV)-hydrates and the photocatalysis of anatase TiO2 nanoparticles, respectively.展开更多
文摘从纺织厂的工业废水排污口取样,分离筛选出了一株嗜热的高效偶氮染料脱色菌PDR2。通过形态学、生理生化特征以及16S r DNA基因序列分析等方法对该菌株进行鉴定,发现该菌为厌氧芽孢杆菌,与Anoxybacillus sp(GQ471935)同源性最高。此外,分别考察了菌株PDR2在不同碳源、氮源、p H以及培养温度等条件下对偶氮染料直接黑38脱色性能的影响,在此基础上进一步研究了菌株PDR2对不同浓度直接黑38染料的耐受性。结果表明,菌株PDR2在55℃、p H 6.0且以葡萄糖为碳源,牛肉膏和蛋白胨为混合氮源时其脱色效果最佳,且菌株对偶氮染料直接黑38有着较强的耐受能力,在染料浓度低于0.4 g·L^(-1)时其脱色效果良好。在最佳条件下静置培养24 h时,菌株PDR2对0.1 g·L^(-1)直接黑38染料的脱色率高达92.34%,展出了良好的应用潜力。
基金supported by the Ministry of Science and Technology of China (Grant No. 2010CB631004)the Fundamental Research Funds for the Central Universities (Grant Nos. 1112021302, 1106021343, 1116021301)+1 种基金the PAPD and National Natural Science Foundation of China (Grant Nos. 50831004, 11004098, 51171078)the research fund of Key Laboratory for Advanced Technology in Environmental Protection of Jiangsu Province (Grant No. AE201015)
文摘This paper describes the effects of temperature on the complex intermediate processes from the precursor to the fully-crystallized anatase TiO2 nanoparticles in hydrothermal synthesis. The anatase TiO2 nanoparticles were synthesized in a wide temperature range below 230°C. The composition, morphology, and methylene blue (MB) decoloration characteristics of the obtained products were investigated by X-ray diffraction, Fourier transform infrared spectroscope, X-ray photoelectron spectroscope, and scanning and transmission electron microscope. The dehydrating polycondensation of Ti(IV)-hydrates and the decomposition of (NH4)2Ti3O7 intermediates with the temperature increase lead to the direct formation of anatase TiO2 nanoparticles under the hydrothermal environments. The strong MB decoloration of the hydrothermal products obtained at the low (≤130°C) and high (≥180°C) temperatures are attributed to the adsorption of Ti(IV)-hydrates and the photocatalysis of anatase TiO2 nanoparticles, respectively.