Barium titanate nano-powders were synthesized under defined conditions. The surface of these particles was successfully modified by coating with urea. The characteristics of these composite particles were studied by X...Barium titanate nano-powders were synthesized under defined conditions. The surface of these particles was successfully modified by coating with urea. The characteristics of these composite particles were studied by X-ray diffraction,transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. The electro-rheological (ER) effects of these particles suspended in methyl-silicone oil were measured. The particle,methyl-silicon oil ratio was 30%-35% weight percent. The experimental results indicate that these ER particles exhibit a remarkable ER effect. The ER fluid shows Bingham characteristics and the static shearing stress increases with an increase of the electric field strength. The highest static shearing stress under a 4 MV/m electric field is 13.2 kPa at room temperature,an increase of about 8.7 kPa compared to untreated BaTiO(C2O4)2 powders.展开更多
The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-i...The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-induced diabetic rats. Three days after diabetes induction, ZCME was administered orally for six weeks （700 mg/kg bw/day）. Serum glucose and lipid profile were evaluated. Reduced glutathione （GSH）, catalase （CAT） and thiobarbituric acid reactive substances （TBARS） were measured in heart tissue. The results showed increased levels of blood glucose, total cholesterol （TC）, LDL cholesterol （LDL-C） and triglyceride （TG） in the diabetic rats. On the other hand, the level of HDL cholesterol （HDL-C） decreased. Compared to the control normal rats, the level of TBARS in heart tissue was markedly increased while GSH and CAT were significantly modified in the diabetic rats. Oral administration of ZCME has normalized serum glucose and lipid profile. TBARS were significantly reduced in heart while CAT and GSH were markedly normalized. Myocardium sections showed the absence of histological changes observed in the diabetic rats. The study suggests that Zygophyllum cornutum may provide a useful therapeutic option in the reversal of metabolic disturbances and oxidative stress-induced cardiac dysfunction in diabetes mellitus.展开更多
OBJECTIVE: To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones. METHODS: Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were se...OBJECTIVE: To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones. METHODS: Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed. RESULTS: Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine. CONCLUSION: These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.展开更多
To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out ...To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance determining region) gyrA, gyrB, parC and parE were amplified by PCR Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed Results Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum展开更多
OBJECTIVE:To investigate the dynamic changes of urinary nephrin,and the relationship between it and urinary albumin excretion rate(UAER) in a diabetic rat model,as well the effects of yiqiyangyinhuayutongluo recipe.ME...OBJECTIVE:To investigate the dynamic changes of urinary nephrin,and the relationship between it and urinary albumin excretion rate(UAER) in a diabetic rat model,as well the effects of yiqiyangyinhuayutongluo recipe.METHODS:Diabetic model was induced by high fat diet combined with low-dose Streptozotocin(STZ) in rats.Normal group(NG),model group(MG),and yiqiyangyinhuayutongluo recipe treated group(YHTG) were set.Gastrointestinal Yiqiyangyinhuayutongluo recipe was administered once daily for 32 w.At the end of the 2nd w(2w),8w,16w,and 32w,fasting blood glucose(FBG),UAER and 24h urinary nephrin(U-nephrin) were detected.RESULTS:Compared with NG,FBG in MG increased notably(P<0.05).Compared with MG,FBG of YHTG reduced slowly,and the difference was significant(P<0.05) since 16w.U-nephrin and UAER in MG increased significantly from 2w,peaked at 16w,lessened in different degree at 32w,but were still higher than NG.The correlation analysis showed that there was a significant positive correlation between U-nephrin and UAER at different time,the correlation coefficient as r>0.9,and P<0.05.Compared with MG,U-nephrin and UAER in YHTG decreased markedly(P<0.05) except for U-nephrin at 8w.CONCLUSIONS:U-nephrin and UAER in diabetic rat model have a positive linear correlation.Yiqiyangyinhuayutongluo recipe can reduce UAER markedly,and preventing the lose of nephrin in urine maybe one of the mechanisms.展开更多
基金Projects BK2005019 supported by the National Science Foundation of Jiangsu Province2005B032 by the Scientific Research Foundation of China University of Mining & Technology
文摘Barium titanate nano-powders were synthesized under defined conditions. The surface of these particles was successfully modified by coating with urea. The characteristics of these composite particles were studied by X-ray diffraction,transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy. The electro-rheological (ER) effects of these particles suspended in methyl-silicone oil were measured. The particle,methyl-silicon oil ratio was 30%-35% weight percent. The experimental results indicate that these ER particles exhibit a remarkable ER effect. The ER fluid shows Bingham characteristics and the static shearing stress increases with an increase of the electric field strength. The highest static shearing stress under a 4 MV/m electric field is 13.2 kPa at room temperature,an increase of about 8.7 kPa compared to untreated BaTiO(C2O4)2 powders.
文摘The present study depicts the therapeutic effect of Zygophyllum cornutum methanolic extract （ZCME） on metabolic disturbances, oxidative stress in heart and myocardium histological changes in streptozotocin （STZ）-induced diabetic rats. Three days after diabetes induction, ZCME was administered orally for six weeks （700 mg/kg bw/day）. Serum glucose and lipid profile were evaluated. Reduced glutathione （GSH）, catalase （CAT） and thiobarbituric acid reactive substances （TBARS） were measured in heart tissue. The results showed increased levels of blood glucose, total cholesterol （TC）, LDL cholesterol （LDL-C） and triglyceride （TG） in the diabetic rats. On the other hand, the level of HDL cholesterol （HDL-C） decreased. Compared to the control normal rats, the level of TBARS in heart tissue was markedly increased while GSH and CAT were significantly modified in the diabetic rats. Oral administration of ZCME has normalized serum glucose and lipid profile. TBARS were significantly reduced in heart while CAT and GSH were markedly normalized. Myocardium sections showed the absence of histological changes observed in the diabetic rats. The study suggests that Zygophyllum cornutum may provide a useful therapeutic option in the reversal of metabolic disturbances and oxidative stress-induced cardiac dysfunction in diabetes mellitus.
文摘OBJECTIVE: To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones. METHODS: Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed. RESULTS: Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine. CONCLUSION: These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.
基金ThisstudywassupportedbyagrantfromtheEducationCommitteeofHunanProvince (No 0 0A0 0 9)
文摘To study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones Methods Thirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance determining region) gyrA, gyrB, parC and parE were amplified by PCR Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed Results Sequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine Conclusion These results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum
基金Supported by the Natural Science Foundation of Hebei (No.C2008001074)
文摘OBJECTIVE:To investigate the dynamic changes of urinary nephrin,and the relationship between it and urinary albumin excretion rate(UAER) in a diabetic rat model,as well the effects of yiqiyangyinhuayutongluo recipe.METHODS:Diabetic model was induced by high fat diet combined with low-dose Streptozotocin(STZ) in rats.Normal group(NG),model group(MG),and yiqiyangyinhuayutongluo recipe treated group(YHTG) were set.Gastrointestinal Yiqiyangyinhuayutongluo recipe was administered once daily for 32 w.At the end of the 2nd w(2w),8w,16w,and 32w,fasting blood glucose(FBG),UAER and 24h urinary nephrin(U-nephrin) were detected.RESULTS:Compared with NG,FBG in MG increased notably(P<0.05).Compared with MG,FBG of YHTG reduced slowly,and the difference was significant(P<0.05) since 16w.U-nephrin and UAER in MG increased significantly from 2w,peaked at 16w,lessened in different degree at 32w,but were still higher than NG.The correlation analysis showed that there was a significant positive correlation between U-nephrin and UAER at different time,the correlation coefficient as r>0.9,and P<0.05.Compared with MG,U-nephrin and UAER in YHTG decreased markedly(P<0.05) except for U-nephrin at 8w.CONCLUSIONS:U-nephrin and UAER in diabetic rat model have a positive linear correlation.Yiqiyangyinhuayutongluo recipe can reduce UAER markedly,and preventing the lose of nephrin in urine maybe one of the mechanisms.
