AIM: To investigate changes in numbers and proliferative function of splenic lymphocytes in patients with hypersplenism due to portal hypertension (PH), to provide evidence for further study of immune status of the sp...AIM: To investigate changes in numbers and proliferative function of splenic lymphocytes in patients with hypersplenism due to portal hypertension (PH), to provide evidence for further study of immune status of the spleen during PH. METHODS: Twelve spleens from patients with hypersplenism due to PH served as the PH group, and four spleens from cases of traumatic spleen rupture were regarded as the control group. After weighing the spleen, lymphocytes were separated and counted using a cell counting plate to calculate the lymphocyte count per gram of spleen tissue (relative quantity) and total lymphocyte count in whole spleen (absolute quantity). The immunohistochemical SP method was used to observe the density and distribution of lymphocytes in the spleen. The MTT method was used to observe changes in lymphocyte proliferative function. RESULTS: As compared to the control group, the splenic lymphocytes in the PH group showed that: (1) There was no difference in distribution but a significant decreasein density; (2) the number of lymphocytes per gram of spleen (relative quantity) decreased significantly (0.822 ± 0.157) × 108 vs (1.174 ± 0.254) × 108, P < 0.01]; (3) with the significant increase in the weight of the PH spleen (832.6 ± 278.2 g vs 211.7 ± 85.6 g, P < 0.01), the total quantity of lymphocytes (absolute quantity) increased significantly (0.685 ± 0.072) × 1011 vs (0.366 ± 0.057) × 1011, P < 0.01]; and (4) the proliferative function of lymphocytes was enhanced: T lymphocytes, (0.022 ± 0.005 vs 0.015 ± 0.003, P < 0.05), and B lymphocytes (0.034 ± 0.006 vs 0.023 ± 0.001, P < 0.01). CONCLUSION: Although lymphocyte density in the spleen decreased in patients with PH, the total quantity of lymphocytes increased because spleen weight increased greatly, along with the proliferating function. With respect to changes in lymphocytes, PH spleens may still have immune function, although it may be disordered. However, complete evaluation of the immune function of the spleen in PH requires more research.展开更多
The spleen could be considered a neglected organ.To date,it has been deemed an ancillary organ in portal hypertension or an organ localization in lymphoproliferative diseases,even though it has had significant attenti...The spleen could be considered a neglected organ.To date,it has been deemed an ancillary organ in portal hypertension or an organ localization in lymphoproliferative diseases,even though it has had significant attention in infectious diseases for some time.Now,it is thought to be central in regulating the immune system,a metabolic asset and involved in endocrine function with regard to nonalcoholic fatty liver disease.The main mechanisms involved in this complex network will be critically discussed in this article.展开更多
The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PC...The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PCF or/and DEX for a given time, the proliferative response of thymocytes and splenoeytes to ConA were measured bv MTF assay; the subpopulations of thymocytes and splenic T lymphoeytes was analyzed by flow cytomety; the cytotoxicity of natural killer cells was measured by Llactate dehydrogenase （LDH） assay; the phagocytosis of rat peritoneal macrophages was measured by Neutral red assay and the Bel-2 protein expression of macrophages was detected by imrnunocytoehemical stain. The proliferative ability of rat thymocytes and splenocytes induced with ConA was enhanced and the depression of lymphoproliferation caused by DEX was reversed by PCF. The percentages of mouse thymic L3 T4^- Lyt-2^- and Lyt-2^＋ subpopulations and splenic Lyt-2 ^＋ cells were decreased and the percentage of splenic L3 T4^ ＋ cells was increased by PCF. The NK cytotoxicity, phagocytosis of macraphages and Bcl-2 protein expression of macrophages were enhanced and the decrease of NK cytotoxicity and Bel-2 protein expression of maerophages caused by DEX were reversed by PCF. PCF could not only enhance the normal immunity function, but also reverse the imrnunosuppression induced by DEX.展开更多
基金The Support Project for talented man in new century from Ministry of Education of People’s Republic of China 2004, No. NCET-04-0932the Project of Tackle Key Problems in Science and Technology of Shaanxi Province, No. 2004K14-G1(4), 2006K14-G2(4)
文摘AIM: To investigate changes in numbers and proliferative function of splenic lymphocytes in patients with hypersplenism due to portal hypertension (PH), to provide evidence for further study of immune status of the spleen during PH. METHODS: Twelve spleens from patients with hypersplenism due to PH served as the PH group, and four spleens from cases of traumatic spleen rupture were regarded as the control group. After weighing the spleen, lymphocytes were separated and counted using a cell counting plate to calculate the lymphocyte count per gram of spleen tissue (relative quantity) and total lymphocyte count in whole spleen (absolute quantity). The immunohistochemical SP method was used to observe the density and distribution of lymphocytes in the spleen. The MTT method was used to observe changes in lymphocyte proliferative function. RESULTS: As compared to the control group, the splenic lymphocytes in the PH group showed that: (1) There was no difference in distribution but a significant decreasein density; (2) the number of lymphocytes per gram of spleen (relative quantity) decreased significantly (0.822 ± 0.157) × 108 vs (1.174 ± 0.254) × 108, P < 0.01]; (3) with the significant increase in the weight of the PH spleen (832.6 ± 278.2 g vs 211.7 ± 85.6 g, P < 0.01), the total quantity of lymphocytes (absolute quantity) increased significantly (0.685 ± 0.072) × 1011 vs (0.366 ± 0.057) × 1011, P < 0.01]; and (4) the proliferative function of lymphocytes was enhanced: T lymphocytes, (0.022 ± 0.005 vs 0.015 ± 0.003, P < 0.05), and B lymphocytes (0.034 ± 0.006 vs 0.023 ± 0.001, P < 0.01). CONCLUSION: Although lymphocyte density in the spleen decreased in patients with PH, the total quantity of lymphocytes increased because spleen weight increased greatly, along with the proliferating function. With respect to changes in lymphocytes, PH spleens may still have immune function, although it may be disordered. However, complete evaluation of the immune function of the spleen in PH requires more research.
文摘The spleen could be considered a neglected organ.To date,it has been deemed an ancillary organ in portal hypertension or an organ localization in lymphoproliferative diseases,even though it has had significant attention in infectious diseases for some time.Now,it is thought to be central in regulating the immune system,a metabolic asset and involved in endocrine function with regard to nonalcoholic fatty liver disease.The main mechanisms involved in this complex network will be critically discussed in this article.
文摘The aim of this study was to investigate the effects of polypeptides from Chlamys farreri （PCF） on immunoeytes or immunocytes treated with dexamethasone （DEX） in vitro. After incubating immunoeytes with 25 mg/L PCF or/and DEX for a given time, the proliferative response of thymocytes and splenoeytes to ConA were measured bv MTF assay; the subpopulations of thymocytes and splenic T lymphoeytes was analyzed by flow cytomety; the cytotoxicity of natural killer cells was measured by Llactate dehydrogenase （LDH） assay; the phagocytosis of rat peritoneal macrophages was measured by Neutral red assay and the Bel-2 protein expression of macrophages was detected by imrnunocytoehemical stain. The proliferative ability of rat thymocytes and splenocytes induced with ConA was enhanced and the depression of lymphoproliferation caused by DEX was reversed by PCF. The percentages of mouse thymic L3 T4^- Lyt-2^- and Lyt-2^＋ subpopulations and splenic Lyt-2 ^＋ cells were decreased and the percentage of splenic L3 T4^ ＋ cells was increased by PCF. The NK cytotoxicity, phagocytosis of macraphages and Bcl-2 protein expression of macrophages were enhanced and the decrease of NK cytotoxicity and Bel-2 protein expression of maerophages caused by DEX were reversed by PCF. PCF could not only enhance the normal immunity function, but also reverse the imrnunosuppression induced by DEX.
