A1M： To investigate the prevalence of celiac disease （CD） as well as CD marker antibodies and susceptibility HLA-DQ haplotypes in 134 karyotyped Down＇s syndrome （DS） patients. METHODS： Immunoglobulin A （IgA） ...A1M： To investigate the prevalence of celiac disease （CD） as well as CD marker antibodies and susceptibility HLA-DQ haplotypes in 134 karyotyped Down＇s syndrome （DS） patients. METHODS： Immunoglobulin A （IgA） and G （IgG） type anti-gliadin antibodies （AGA）, IgA type anti-tissue transglutaminase （tTG） antibodies （anti-tTG） with antigen of guinea pig and human source were determined by enzyme-linked immunosorbent assay and endomysium antibodies （EHA） by indirect immunofiuoresence test. HLA-DQA1＊0501/DQB1＊0201 （DQ2） was revealed by polymerase chain reaction. Celiac disease was diagnosed by revised ESPGHAN criteria. RESULTS： 41% of DS patients had AGA, 6.0% IgA anti-tTG with guinea pig antigen, and 3.0 % [gA EMA （all positive for anti-tTG with human tTG）. Subtotal villous atrophy was found in 5 out of 9 DS patients who had agreed to small bowel biopsy. One of them had DQA1＊0S01/DQB1＊0201 and anti-tTG and EMA i.e. typical for CD markers （this case also fulfilled the ESPGHAN diagnostic criteria）, but other four lacked these markers. Three non-biopsied DS patients had also most probably CD because DQA1＊0S01/DQB1＊0201 and IgA anti-tTG （EMA） were detected. Thus, the prevalence of CD among our DS patients population is 3.0 % （95 % of confidence interval [CI]： 0.1-5.9 %）. CONCLUSION： We confirm the increased frequency of CD among DS patients. In addition, we have revealed a subgroup of patients with subtotal villous atrophy but without characteristic for CD immunological and genetic markers. Whether these cases represent CD （with atypical immunopathogenesis） or some other immune enteropathy, requires further investigations.展开更多
We studied the effects of nasal thermosensible gels containing Chinese medicine Xingbi on Bufo gargarizans maxillary mucosal cilia movement and the ciliotoxicity in rats nasal mucosa. The saline water was used as a bl...We studied the effects of nasal thermosensible gels containing Chinese medicine Xingbi on Bufo gargarizans maxillary mucosal cilia movement and the ciliotoxicity in rats nasal mucosa. The saline water was used as a blank control, and 1% hydrochloric acid of methamphetamine Massachusetts was used as the negative control. Compared with normal saline control, the relative percentage of the lasting time of ciliary movement treated with Chinese medicine Xingbi was 94.1%. There was no remarkable pathological change in the tissue slice of nasal mucosa, and no stimulation on nasal mucous membrane was observed. So these data suggest that nasal thermosensible gel of Chinese medicine Xingbi is of high safety. It has no damage to the mucosa of toads and rats and can be used for intranasal administration.展开更多
基金Supported by Estonian Science Foundation grants No. 4437 and 6514.
文摘A1M： To investigate the prevalence of celiac disease （CD） as well as CD marker antibodies and susceptibility HLA-DQ haplotypes in 134 karyotyped Down＇s syndrome （DS） patients. METHODS： Immunoglobulin A （IgA） and G （IgG） type anti-gliadin antibodies （AGA）, IgA type anti-tissue transglutaminase （tTG） antibodies （anti-tTG） with antigen of guinea pig and human source were determined by enzyme-linked immunosorbent assay and endomysium antibodies （EHA） by indirect immunofiuoresence test. HLA-DQA1＊0501/DQB1＊0201 （DQ2） was revealed by polymerase chain reaction. Celiac disease was diagnosed by revised ESPGHAN criteria. RESULTS： 41% of DS patients had AGA, 6.0% IgA anti-tTG with guinea pig antigen, and 3.0 % [gA EMA （all positive for anti-tTG with human tTG）. Subtotal villous atrophy was found in 5 out of 9 DS patients who had agreed to small bowel biopsy. One of them had DQA1＊0S01/DQB1＊0201 and anti-tTG and EMA i.e. typical for CD markers （this case also fulfilled the ESPGHAN diagnostic criteria）, but other four lacked these markers. Three non-biopsied DS patients had also most probably CD because DQA1＊0S01/DQB1＊0201 and IgA anti-tTG （EMA） were detected. Thus, the prevalence of CD among our DS patients population is 3.0 % （95 % of confidence interval [CI]： 0.1-5.9 %）. CONCLUSION： We confirm the increased frequency of CD among DS patients. In addition, we have revealed a subgroup of patients with subtotal villous atrophy but without characteristic for CD immunological and genetic markers. Whether these cases represent CD （with atypical immunopathogenesis） or some other immune enteropathy, requires further investigations.
基金National Natural Science Foundation of China (Grant No.81073117)the construction ofcollege service routines in fujian province key project(Grant No.2008FG-06)
文摘We studied the effects of nasal thermosensible gels containing Chinese medicine Xingbi on Bufo gargarizans maxillary mucosal cilia movement and the ciliotoxicity in rats nasal mucosa. The saline water was used as a blank control, and 1% hydrochloric acid of methamphetamine Massachusetts was used as the negative control. Compared with normal saline control, the relative percentage of the lasting time of ciliary movement treated with Chinese medicine Xingbi was 94.1%. There was no remarkable pathological change in the tissue slice of nasal mucosa, and no stimulation on nasal mucous membrane was observed. So these data suggest that nasal thermosensible gel of Chinese medicine Xingbi is of high safety. It has no damage to the mucosa of toads and rats and can be used for intranasal administration.
