目的:建立骨形态发生蛋白4(bone morphogenetic protein 4,BMP4)、脱钙骨基质和切断跟腱诱导的异位骨化动物模型,并初步探讨其形成机制,为异位骨化的研究奠定实验基础。方法:制备动物模型:(1)构建BMP4重组腺病毒,实验组裸鼠的一侧腓肠...目的:建立骨形态发生蛋白4(bone morphogenetic protein 4,BMP4)、脱钙骨基质和切断跟腱诱导的异位骨化动物模型,并初步探讨其形成机制,为异位骨化的研究奠定实验基础。方法:制备动物模型:(1)构建BMP4重组腺病毒,实验组裸鼠的一侧腓肠肌内注入50μl1×107pfu BMP4重组腺病毒液,对照组腓肠肌内注入50μl1×107pfu空病毒液。4周后行X线和组织学检查。(2)无菌条件下,股后外侧入路,将50mg脱钙骨基质植入裸鼠股后肌群内,4周后行X线和组织学检查。(3)20只小鼠于跟腱中点行跟腱切断术,10周后行X线和组织学检查。结果:X线和组织学检查显示4周后,注射BMP4重组腺病毒的动物均出现异位骨,注射空病毒组未见异位骨形成。4周后,植入脱钙骨基质的动物均出现异位骨。10周后,行跟腱切断术的动物均在跟腱部位出现异位骨。结论:BMP4、脱钙骨基质和切断跟腱可有效诱导异位骨化,结果稳定可靠。展开更多
AIM:To introduce the importance of foot abductor splint on conservative treatment of congenital talipes equinovarus. METHODS:A total of 48 patients with congenital talipes equinovarus(60 feet)(5 days -6 months) in the...AIM:To introduce the importance of foot abductor splint on conservative treatment of congenital talipes equinovarus. METHODS:A total of 48 patients with congenital talipes equinovarus(60 feet)(5 days -6 months) in the Department of Orthopaedics,Shanghai Children’s Medical Center,from January 2000 to May 2003,were given conservative treatment.Successive plaster reshaping and subcutaneous achillotenotomy was performed with Posenti method.All patients wore foot abductor splint with the course of 2 years.The first 3 months was full time wearing and the others only sleeping time wearing. RESULTS:All cases were followed up(with the mean follow up period of 14.68 months).Results were satisfactory from the evaluation of X ray and clinical situation. Dimglio score type:46 cases were Type Ⅰ,and 2 cases were TypeⅡa.The age when the manipulation and cast started,the maximum dorsiflexion of the ankle when the splint was started,and the period of the full time wearing in the changes of data in X ray imaging were not related to each other.However,the age when the splint was started was significantly associated with the maximum dorsiflexion of the ankle and the tibio calcaneal angle.CONCLUSION:The foot abductor splint is an indispensably important part for conservative treatment of congenital talipes euinovarus,and is effective to prevent the relapse and maintain good clinical results.展开更多
[目的]对比分析合并肩袖损伤的肱二头肌长头腱(long head of biceps tendon,LHBT)病变固定与切断术的疗效.[方法]回顾性分析2018年1月-2020年6月关节镜治疗合并肩袖损伤的LHBT病变30例患者的临床资料.其中,11例行LHBT固定术(固定组),19...[目的]对比分析合并肩袖损伤的肱二头肌长头腱(long head of biceps tendon,LHBT)病变固定与切断术的疗效.[方法]回顾性分析2018年1月-2020年6月关节镜治疗合并肩袖损伤的LHBT病变30例患者的临床资料.其中,11例行LHBT固定术(固定组),19例行LHBT切断术(切断组),两组均行肩袖修补.比较两组近期临床效果.[结果]两组患者均顺利完成手术,无严重并发症,两组手术时间、切口长度、失血量的差异均无统计学意义(P>0.05).与术前相比,末次随访时两组VAS评分均显著降低,而ASES评分显著增加(P<0.05);但是,相应时间点两组间VAS和ASES评分的差异均无统计学意义(P>0.05).单纯切断组出现1例Popeye畸形,术后两组患者屈伸肘部肌力无明显差异,肩关节主被动活动度无显著障碍.[结论]对合并肩袖损伤的LHBT病变治疗,LHBT固定术与LHBT切断术联合肩袖修补均有显著的近期临床疗效,但单纯腱切断术后有Popeye畸形发生可能.展开更多
文摘目的:建立骨形态发生蛋白4(bone morphogenetic protein 4,BMP4)、脱钙骨基质和切断跟腱诱导的异位骨化动物模型,并初步探讨其形成机制,为异位骨化的研究奠定实验基础。方法:制备动物模型:(1)构建BMP4重组腺病毒,实验组裸鼠的一侧腓肠肌内注入50μl1×107pfu BMP4重组腺病毒液,对照组腓肠肌内注入50μl1×107pfu空病毒液。4周后行X线和组织学检查。(2)无菌条件下,股后外侧入路,将50mg脱钙骨基质植入裸鼠股后肌群内,4周后行X线和组织学检查。(3)20只小鼠于跟腱中点行跟腱切断术,10周后行X线和组织学检查。结果:X线和组织学检查显示4周后,注射BMP4重组腺病毒的动物均出现异位骨,注射空病毒组未见异位骨形成。4周后,植入脱钙骨基质的动物均出现异位骨。10周后,行跟腱切断术的动物均在跟腱部位出现异位骨。结论:BMP4、脱钙骨基质和切断跟腱可有效诱导异位骨化,结果稳定可靠。
文摘AIM:To introduce the importance of foot abductor splint on conservative treatment of congenital talipes equinovarus. METHODS:A total of 48 patients with congenital talipes equinovarus(60 feet)(5 days -6 months) in the Department of Orthopaedics,Shanghai Children’s Medical Center,from January 2000 to May 2003,were given conservative treatment.Successive plaster reshaping and subcutaneous achillotenotomy was performed with Posenti method.All patients wore foot abductor splint with the course of 2 years.The first 3 months was full time wearing and the others only sleeping time wearing. RESULTS:All cases were followed up(with the mean follow up period of 14.68 months).Results were satisfactory from the evaluation of X ray and clinical situation. Dimglio score type:46 cases were Type Ⅰ,and 2 cases were TypeⅡa.The age when the manipulation and cast started,the maximum dorsiflexion of the ankle when the splint was started,and the period of the full time wearing in the changes of data in X ray imaging were not related to each other.However,the age when the splint was started was significantly associated with the maximum dorsiflexion of the ankle and the tibio calcaneal angle.CONCLUSION:The foot abductor splint is an indispensably important part for conservative treatment of congenital talipes euinovarus,and is effective to prevent the relapse and maintain good clinical results.
文摘[目的]对比分析合并肩袖损伤的肱二头肌长头腱(long head of biceps tendon,LHBT)病变固定与切断术的疗效.[方法]回顾性分析2018年1月-2020年6月关节镜治疗合并肩袖损伤的LHBT病变30例患者的临床资料.其中,11例行LHBT固定术(固定组),19例行LHBT切断术(切断组),两组均行肩袖修补.比较两组近期临床效果.[结果]两组患者均顺利完成手术,无严重并发症,两组手术时间、切口长度、失血量的差异均无统计学意义(P>0.05).与术前相比,末次随访时两组VAS评分均显著降低,而ASES评分显著增加(P<0.05);但是,相应时间点两组间VAS和ASES评分的差异均无统计学意义(P>0.05).单纯切断组出现1例Popeye畸形,术后两组患者屈伸肘部肌力无明显差异,肩关节主被动活动度无显著障碍.[结论]对合并肩袖损伤的LHBT病变治疗,LHBT固定术与LHBT切断术联合肩袖修补均有显著的近期临床疗效,但单纯腱切断术后有Popeye畸形发生可能.
