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培美曲塞联合奥沙利铂治疗肺癌的疗效与机制研究 被引量:6
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作者 王英 《中国现代医学杂志》 CAS 2019年第19期53-57,共5页
目的研究培美曲塞联合奥沙利铂调控NOD1/NF-κB/R1P2信号治疗肺癌的机制。方法选取2014年1月—2017年1月天津市第五中心医院收治的晚期肺腺癌患者80例。随机分为两组:观察组接受培美曲塞联合奥沙利铂化疗;对照组接受培美曲塞联合顺铂化... 目的研究培美曲塞联合奥沙利铂调控NOD1/NF-κB/R1P2信号治疗肺癌的机制。方法选取2014年1月—2017年1月天津市第五中心医院收治的晚期肺腺癌患者80例。随机分为两组:观察组接受培美曲塞联合奥沙利铂化疗;对照组接受培美曲塞联合顺铂化疗。化疗2个疗程后评价疗效和不良反应,采用酶联免疫吸附试验(ELISA)检测患者血清NOD1、NF-κB、R1P2及Caspase-3蛋白含量;采用实时聚合酶链反应(real-time PCR)检测肺腺癌组织NOD1、NF-κB、R1P2及Caspase-3 mRNA表达。结果观察组培美曲塞联合奥沙利铂化疗的效果优于对照组(P<0.05);观察组培美曲塞联合奥沙利铂化疗的不良反应与对照组比较,差异有统计学意义(P <0.05),观察组低于对照组;与化疗前比较,化疗后观察组和对照组患者血清NOD1、NF-κB、R1P2和Caspase-3蛋白含量升高,癌组织NOD1、NF-κB、R1P2及Caspase-3m RNA表达升高(P<0.05);与对照组比较,观察组患者血清NOD1、NF-κB、R1P2及Caspase-3蛋白含量升高,癌组织NOD1、NF-κB、R1P2及Caspase-3 mRNA表达升高(P<0.05)。结论培美曲塞联合奥沙利铂治疗肺腺癌与联合顺铂比较,其疗效更高、不良反应更小,其机制可能与调控NOD1/NF-κB/R1P2信号有关。 展开更多
关键词 腺癌/肺肿瘤 培美曲塞/药物疗法 联合 奥沙利铂/药物疗法 联合 NOD1/NF-κB/R1P2
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裸鼠糖尿病合并肺腺癌模型建立
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作者 邵倩 李建彬 +5 位作者 刘珊珊 王玮 梅慧 张英杰 徐敏 任爱军 《中华肿瘤防治杂志》 CAS 北大核心 2015年第24期1874-1878,共5页
目的 糖尿病和肺癌均为常见病、多发病,发病率逐年升高,糖尿病合并肺癌的发病率亦明显升高。本研究通过制备裸鼠糖尿病、肺腺癌和糖尿病合并肺腺癌模型,探讨糖尿病和肺癌之间的关系。方法 选择4~6周龄雄性裸鼠54只,体质量18~22g,随机分... 目的 糖尿病和肺癌均为常见病、多发病,发病率逐年升高,糖尿病合并肺癌的发病率亦明显升高。本研究通过制备裸鼠糖尿病、肺腺癌和糖尿病合并肺腺癌模型,探讨糖尿病和肺癌之间的关系。方法 选择4~6周龄雄性裸鼠54只,体质量18~22g,随机分为正常对照组(12只)、糖尿病组(12只)、肺腺癌组(15只)和糖尿病合并肺腺癌组(15只)4组。制备糖尿病模型:裸鼠腹腔内注射链脲佐菌素(STZ)150mg/kg(根据预实验结果得出)。制备肺腺癌模型:收集培养的A549细胞,计数,调整细胞悬液浓度为1×107个/mL,以每只0.2mL接种于裸小鼠右侧腋窝皮下,正常对照组腹腔内及腋下均注射等量生理盐水。待糖尿病造模成功并稳定后,再在裸鼠腋部皮下注射人肺腺癌细胞株,制备糖尿病合并肺腺癌模型。结果 裸鼠糖尿病、肺腺癌、糖尿病合并肺腺癌模型均造模成功,其中体质量、血糖在正常对照组、肺腺癌组、糖尿病组及糖尿病合并肺腺癌组开始时差异无统计学意义;造模成功后,肺腺癌组、糖尿病组及糖尿病合并肺腺癌组体质量在放疗前、放疗后1和2个月明显低于正常对照组,P〈0.05;血糖在糖尿病组、糖尿病合并肺腺癌组明显高于正常对照组、肺腺癌组,P〈0.05;至放疗前,正常对照组、糖尿病组、肺腺癌组、糖尿病合并肺腺癌组各组自然死亡率差异无统计学意义,P〉0.05,均死亡1只。肿瘤标志物癌胚抗原(CEA)在肺腺癌组及糖尿病合并肺腺癌组放疗前后差异有统计学意义,P〈0.05;且明显高于正常对照组及糖尿病组,P〈0.05。结论 裸鼠糖尿病、肺腺癌和糖尿病合并肺腺癌模型的成功建立,为临床糖尿病、肺腺癌及糖尿病合并肺腺癌的治疗提供实验依据。 展开更多
关键词 糖尿病 腺癌/肺 裸鼠 模型
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Deep learning model based on PET/CT and combination with Cox proportional hazard model for predicting progression of lung invasive adenocarcinoma after surgery
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作者 LI Yingci WU Dongbo GONG Feifei 《中国医学影像技术》 CSCD 北大核心 2024年第8期1194-1198,共5页
Objective To observe the efficacy of deep learning(DL)model based on PET/CT and its combination with Cox proportional hazard model for predicting progressive disease(PD)of lung invasive adenocarcinoma within 5 years a... Objective To observe the efficacy of deep learning(DL)model based on PET/CT and its combination with Cox proportional hazard model for predicting progressive disease(PD)of lung invasive adenocarcinoma within 5 years after surgery.Methods The clinical,PET/CT and 5-year follow-up data of 250 patients with lung invasive adenocarcinoma were retrospectively analyzed.According to PD or not,the patients were divided into the PD group(n=71)and non-PD group(n=179).The basic data and PET/CT findings were compared between groups,among which the quantitative variables being significant different between groups were transformed to categorical variables using receiver operating characteristic(ROC)curve and corresponding cut-off value.Multivariant Cox proportional hazard model was used to select independent predicting factors of PD of lung invasive adenocarcinoma within 5 years after surgery.The patients were divided into training,validation and test sets at the ratio of 6∶2∶2,and PET/CT data in training set and validation set were used to train model and tuning parameters to build the PET/CT DL model,and the combination model was built in serial connection of DL model and the predictive factors.In test set,the efficacy of each model for predicting PD of lung invasive adenocarcinoma within 5 years after surgery was assessed and compared using the area under the curve(AUC).Results Patients'gender and smoking status,as well as the long diameter,SUV max and SUV mean of lesions measured on PET images,the long diameter,short diameter and type of lesions showed on CT were statistically different between groups(all P<0.05).Smoking(HR=1.787[1.053,3.031],P=0.031)and lesion SUV max>4.15(HR=5.249[1.062,25.945],P=0.042)were both predictors of PD of lung invasive adenocarcinoma within 5 years after surgery.In test set,the AUC of PET/CT DL model for predicting PD was 0.847,of the combination model was 0.890,of the latter was higher than of the former(P=0.036).Conclusion DL model based on PET/CT had high efficacy for predicting PD of lung invasive adenocarcinoma within 5 years after surgery.Combining with Cox proportional hazard model could further improve its predicting efficacy. 展开更多
关键词 adenocarcinoma of lung positron-emission tomography and computed tomography deep learning disease progression
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Comparative Study of CT Images of Cerebral Metastasis from Pulmonary Squamous Cell Carcinoma and Adenocarcinoma
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作者 高德培 丁莹莹 谭静 《The Chinese-German Journal of Clinical Oncology》 CAS 2004年第1期34-36,66,共4页
Objective: To explore the di?erence of CT images of metastatic tumors to the brain of lung squamous cell cancer and lung glandular cancer. Methods: Forty-?ve cases of lung squamous cell cancer an... Objective: To explore the di?erence of CT images of metastatic tumors to the brain of lung squamous cell cancer and lung glandular cancer. Methods: Forty-?ve cases of lung squamous cell cancer and 59 cases of lung glandular cancer have gone through pathological con?rmation and all the 104 cases have been put under enhanced scanning on the basis of ?at scanning. The shape and density of the metastatic tumors, encephaledema around the tumors, and the reinforcement change after the enhancement were analyzed. Results: The CT scanning images of all the cases showed that, under ?at scanning, the density of the metastatic tumors to the brain of lung squamous cell cancer was over the slightly-high level and by no means even. Again, under ?at scanning, some 10 cases of metastatic tumors to the brain of lung glandular cancer showed a slightly-high density and some 49 other cases a low density and sack-like low density. Forty-?ve cases of metastatic tumors to the brain of lung squamous cell cancer were with hydroncus while some 30 cases of that of lung glandular cancer were in the same condition. Most of the two types of metastatic tumors, amounting to 97 cases, took place in supratentorial manner, and only 7 cases took place in subtentorial manner. Conclusion: The CT images of metastatic tumors to the brain of lung squamous cell cancer and lung glandular cancer are obviously di?erent from each other. As for the lung squamous cell cancer, the ?at scanning density of the tumor is over the slightly-high level with unevenness. There is relatively obvious reinforcement and obvious hydroncus. As for the lung glandular cancer, the metastatic tumor to the brain shows a low density and sack-like low density under ?at scanning. There is no obvious reinforcement and relatively slight hydroncus. 展开更多
关键词 tumor lung metastatic tumor brain tomography X-ray computer
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Characterization of Two Human Lung Adenocarcinoma Cell Lines by Reciprocal Chromosome Painting 被引量:4
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作者 彭昆靖 王金焕 +3 位作者 苏伟婷 王熙才 杨凤堂 佴文惠 《Zoological Research》 CAS CSCD 北大核心 2010年第2期113-121,共9页
Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established fro... Lung cancer is a leading cause of cancer death worldwide. Some lung cancer patients correlate with a gas of radon besides smoking. To search for common chromosomal aberrations in lung cancer cell lines established from patients induced by different factors, a combined approach of chromosome sorting, forward and reverse chromosome painting was used to characterize karyotypes of two lung adenocarcinoma cell lines: A549 and GLC-82 with the latter line derived from a patient who has suffered long-term exposure to environmental radon gas pollution. The chromosome painting results revealed that complex chromosomal rearrangements occurred in these two lung adenocarcinoma cell lines. Thirteen and twenty-four abnormal chromosomes were identified An A549 and GLC-82 cell lines, respectively. Almost half of abnormal chromosomes in these two cell lines were formed by non-reciprocal translocations, the others were derived from deletions and duplication/or amplification in some chromosomal regions. Furthermore, two apparently common breakpoints, HSA8q24 and 12q14 were found in these two lung cancer cell lines. 展开更多
关键词 Lung adenocarcinoma cell lines Chromosome sorting Chromosome painting Cytogenetic characterization
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Synergistic effect of all-trans-ret inoic acid and arsenic trioxide on growth inhibition and apoptosis in human hepatoma, breast cancer, and lung cancer cells in vitro 被引量:24
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作者 Le-Min Lin Bao-Xin Li +2 位作者 Jian-Bing Xiao Dan-Hua Lin Bao-Feng Yang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第36期5633-5637,共5页
AIM: To investigate the effect of all-trans-retinoic acid (ATRA) on arsenic trioxide (As2O3)-induced apoptosis of human hepatoma, breast cancer, and lung cancer cells in an attempt to find a better combination th... AIM: To investigate the effect of all-trans-retinoic acid (ATRA) on arsenic trioxide (As2O3)-induced apoptosis of human hepatoma, breast cancer, and lung cancer cells in an attempt to find a better combination therapy for solid tumors. METHODS: Human hepatoma cell lines HepG2, Hep3B, human breast cancer cell line MCF-7, and human lung adenocarcinoma cell line AGZY-83-a were treated with As203 together with ATRA. Cell survival fraction was determined by MTT assay, cell viability and apoptosis were measured by annexin V-fluorescein isothiocyanate (FITC) and PI staining, and intracellular glutathione (GSH) and glutathione-S-transferase (GST) activities were determined using commercial kits. RESULTS: Cytotoxicity of ATRA was low. ATRA (0.1, 1, and 10 μmol/L) could synergistically potentiate As2O3 to exert a dose-dependent inhibition of growth and to induce apoptosis in each of the cell lines. HepG2 and Hep3B with low intracellular GSH or GST activities were remarkably sensitive to As2O3 or As2O3+ATRA, while AGZY-83-a with higher GSH or GST activities was less sensitive to As2O3 or As2O3+ATRA. Treatment with 2 μmol/L As2O3 for 72 h significantly decreased intracellular GSH and GST levels in each of the cell lines, and 1 μmol/L ATRA alone reduced minimal intracellular GSH and GST levels. ATRA potentiated the effect of As2O3 on intracellular GSH levels, but intracellular GST levels were not significantly affected by the combination of As2O3 and ATRA for 72 h as compared to As2O3 alone.CONCLUSION: ATRA can strongly potentiate As2O3- induced growth-inhibition and apoptosis in each of the cell lines, and two drugs can produce a significant synergic effect. The sensitivity to As2O3 or As2O3+ATRA is inversely proportional to intracellular GSH or GST levels in each of the cell lines. The GSH redox system may be the possible mechanism by which ATRA synergistically potentiates As203 to exert a dose-dependent inhibition of growth and to induce apoptosis. 2005 The WJG Press and Elsevier Inc. All rights reserved. 展开更多
关键词 Arsenic trioxide AII-trans-retinoic acid Hepatocellular carcinoma Cancer of breast and lung GLUTATHIONE Glutathione-S-tra nsferase
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In vitro study of radiosensitization by β-Elemene in A549 cell line from adenocarcinoma of lung 被引量:18
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作者 Hao Jiang Shenglin Ma Jianguo Feng 《The Chinese-German Journal of Clinical Oncology》 CAS 2009年第1期12-15,共4页
Objective: To explore the effect and possible mechanism in vitro of radiosensitization by β-Elemene in A549 cell line from adenocarcinoma of lung. Methods: The A549 cell line from adenocarcinoma of lung was chosen ... Objective: To explore the effect and possible mechanism in vitro of radiosensitization by β-Elemene in A549 cell line from adenocarcinoma of lung. Methods: The A549 cell line from adenocarcinoma of lung was chosen for the study to determine the inhibition ratio by using MTT assay. Morphologic change, growth curve, cloning efficiency, divisional index were observed. Change of cell cycle and apoptosis rate were analyzed by FCM and the expressions of gene P53 and Bcl-2 were detected. Results: Reproductive activity of the group which was under irradiation and β-Elemene was significantly suppressed and its cloning efficiency and divisional index also declined. The apoptosis rate of the group which was under irradiation and β-Elemene was significantly higher at 48 h and 72 h, which was analyzed by FCM. The expression of P53 without Bcl-2 was observed in the group under irradiation and β-Elemene and the group under β-Elemene only at the 48th hour point, while the expression of Bcl-2 without p53 was observed in the group under irradiation only and the control group. Conclusion: β-Elemene is good at radiosensitization and its mechanism may be relevant to the up-regulation of P53, down-regulation of Bcl-2 and inducing apoptosis. 展开更多
关键词 ELEMENE lung neoplasm RADIOTHERAPY
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Inhibition of expression of vascular endothelial growth factor and its receptors in pulmonary adenocarcinoma cell by TNP-470 in combination with gemcitabine 被引量:5
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作者 WANG Xue-fen TU Ling-fang +1 位作者 WANG Li-hong ZHOU Jian-ying 《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 SCIE CAS CSCD 2006年第10期837-843,共7页
Angiogenesis is required for solid tumor growth and facilitates tumor progression and metastasis. The inhibition effects of O-(chloroacetyl-carbamoyl) fumagillol (TNP-470), an angiogenesis inhibitor, and gemcitabi... Angiogenesis is required for solid tumor growth and facilitates tumor progression and metastasis. The inhibition effects of O-(chloroacetyl-carbamoyl) fumagillol (TNP-470), an angiogenesis inhibitor, and gemcitabine, a chemotherapeutic agent, on expression of growth factors were investigated using human pulmonary adenocarcinoma cell line, A549. The A549 cells were divided into four groups: control group, 10^-6 mg/ml gemcitabine treated group, 10^-4 mg/ml TNP-470 treated group and gemcitabine+TNP-470 treated group. The mRNA and protein expression of vascular endothelial growth factor (VEGF) and its receptors, FMS-like tyrosine kinase-l (FLT-1) and kinase insert domain-containing receptor (KDR), in different groups were measured. The growth of A549 cell cultured with gemcitabine or TNP-470 was inhibited in an almost dose-dependent manner. Although gemcitabine (10^-6 mg/ml) alone and TNP-470 (10^-4 mg/ml) alone had no effect on the mRNA and protein expression of VEGF and its receptors (FLT-1, KDR) in A549 cells compared to the control (P〉0.05), 10^-6 mg/ml gemcitabine in combination with 10^-4 mg/ml TNP-470 had significant effect (P〈0.01). Moreover, combination of the two drugs significantly inhibited the mRNA expression of VEGF, FLT-1 and KDR compared to either drug alone (P〈0.05). This study suggests that combined treatment with TNP-470 plus gemcitabine may augment the antiangiogenic and antineoplastic effects in lung cancer cells in vitro. 展开更多
关键词 TNP-470 GEMCITABINE VEGF FLT-1 KDR
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Correlation of the quantifiable parameters of blood flow pattern derived with dynamic CT in solitary bronchogenic adenocarcinoma 被引量:8
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作者 Shenjiang Li Xiangsheng Xiao +3 位作者 Shiyuan Liu Huimin Li Chengzhou Li Chenshi Zhang 《The Chinese-German Journal of Clinical Oncology》 CAS 2007年第4期345-349,共5页
Objective:To evaluate the correlation of the quantifiable parameters of blood flow pattern derived with dynamic CT in solitary bronchogenic adenocarcinoma(SBA).Methods:46 patients with solitary bronchogenic adenocarci... Objective:To evaluate the correlation of the quantifiable parameters of blood flow pattern derived with dynamic CT in solitary bronchogenic adenocarcinoma(SBA).Methods:46 patients with solitary bronchogenic adenocarcinomas (SBA)(diameter≤4 cm)underwent multi-location dynamic contrast material-enhanced(nonionic contrast material was administrated via the antecubital vein at a rate of 4 mL/s by using an autoinjector 90 mL,4×5 mm or 4×2.5 mm scanning mode with stable table were performed)serial CT.Precontrast and postcontrast attenuation on every scan was recorded.Perfusion (PBA),peak height(PHBA),ratio of peak height of the SPN to that of the aorta(BA-to-A ratio)and mean transit time(MTT)were calculated.The correlation between peak height of the aorta(PHA)and parameters of the SBA(PHBA,BA-to-A ratio,PBA,and MTT)and those among parameters of the SBA were assessed by means of linear regression analysis.Regression equation among parameters of the SBA were obtain by means of stepwise regression.Results:The correlation between the SBA peak height(PHBA,36.78 HU±12.02)and the aortic peak height(PHA)was significant(r=0.506,P<0.0001).No significant cor- relation was found between the BA-to-Apeak height ratio(15.33%±4.55)and the aortic peak height(r=0.130,P=0.388> 0.05)as it was between the SBA perfusion(PBA,31.86 mL/min/100 g±9.74)and the aortic peak height(r=0.049,P=0.749 >0.05).The SBA perfusion correlated with the PHBA and the BA-to-A peak height ratio(r=0.394,P=0.007<0.05;r=0.407, P=0.005<0.05).The PHBA correlated positively with the BA-to-A peak height ratio(r=0.781,P<0.0001).Mean transit time was 14.84 s±5.52.PBA=18.500+0.872×BA-to-A ratio.BA-to-A ratio=4.467+0.295×PHBA.Conclusion:The linear correlation between the SBA perfusion and BA-to-Aratio and that between BA-to-Aratio and PHBA can be expressed by equation. It is possible to design a simpler scanning procedure of investigation of bronchogenic adenocarcinoma angiogenesis. 展开更多
关键词 bronchogenic adenocarcinoma blood flow pattern CT quantifiable parameters linear correlation
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Breast metastasis from lung cancer:a report of two cases and literature review 被引量:3
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作者 Li Wang Shu-Ling Wang Hong-Hong Shen Feng-Ting Niu Yun Niu 《Cancer Biology & Medicine》 CAS CSCD 2014年第3期208-215,共8页
Breast metastasis from extra-mammary malignancy is rare. An incidence of 0.4% to 1.3% has been reported in literature. The primary malignancies that most commonly metastasize to the breast are leukemia, lymphoma, and ... Breast metastasis from extra-mammary malignancy is rare. An incidence of 0.4% to 1.3% has been reported in literature. The primary malignancies that most commonly metastasize to the breast are leukemia, lymphoma, and malignant melanoma. In this report, two cases of pulmonary metastasis to the breast were presented. A 40-year-old female manifested a right breast mass of 2-month duration. After physical examination was performed, a poorly defined mass was noted in the upper outer quadrant of the right breast. Another 49-year-old female manifested right breast mass of 5-day duration. A poorly defined mass was noted in the lower inner quadrant of the right breast. Mammography results also revealed breast cancer. The patients underwent local excision. After histological and immunohistochemical analyses were conducted, a primary lung carcinoma that metastasized to the breast was diagnosed. An accurate differentiation of metastasis to the breast from primary breast cancer is very important because the treatment and prognosis of the two differ significantly. 展开更多
关键词 Lung cancer breast metastasis immunohistochemistry thyroid transcription factor-1(TTF1)
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OVER-EXPRESSION OF THE RAB5 GENE IN HUMAN LUNG ADENOCARCINOMA CELLS WITH HIGH METASTATIC POTENTIAL 被引量:5
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作者 李钰 孟祥文 +3 位作者 冯会臣 张贵寅 刘春祥 李璞 《Chinese Medical Sciences Journal》 CAS CSCD 1999年第2期96-101,共6页
INTRODUCTIONMetastasisisthemajorcauseofdeathfrommanyneoplasias.Butthemolecularmechanismin-volvedinthemetasta... INTRODUCTIONMetastasisisthemajorcauseofdeathfrommanyneoplasias.Butthemolecularmechanismin-volvedinthemetastaticprocessisstilu... 展开更多
关键词 neoplastic metastasis lung cancer RAB5A gene
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ADENOSQUAMOUS LUNG CARCINOMA:CLINICAL CHARACTERISTICS,SURGICAL TREAMENT AND PROGNOSIS 被引量:4
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作者 贲勇 于洪泉 +4 位作者 王振杰 苗齐 任华 张志庸 李泽坚 《Chinese Medical Sciences Journal》 CAS CSCD 2000年第4期238-240,共3页
Objective.The effectiveness of surgical resection of adenosquamous carcinoma of the lung remains poorly defined because of the histology’s relatively low frequency, the failure in most published series to separate ad... Objective.The effectiveness of surgical resection of adenosquamous carcinoma of the lung remains poorly defined because of the histology’s relatively low frequency, the failure in most published series to separate adenosquamous carcinoma from the other variants of non-small cell lung carcinoma.To define the effectiveness of surgical treatment of adenosquamous carcinoma,we have retrospectively reviewed our hospital experience over a 12-year period. Methods.Retrospectively reviewed 22 cases of adenosquamous carcinoma who were surgically treated,except one patient,in the PUMCH from Jan.1985 to Aug.1997.This series constitutes the 19% of a total of 1 245 patients with all types of surgical treatment for the primary lung cancer during the same time. Results.The adenosquanous carcinoma was mostly presented in the old patients with a mean age of 60 years and mostly located in the peripheral of lung(n=20).The overall 5-year survival was 23%.Those with stage Ⅰ tumors survival was only 18%(n=13), stage Ⅱ 5%. The survival in stage Ⅲ tumors was not longer than 25 months and in stage Ⅳ survival was not longer than 12 months. Conclusion.Our results suggest that adenosquamous carcinoma of lung was a virulent tumor,which exhibited highly aggressive biological behavior with early lymph nodes metastasis(46%) and its prognosis was worse than that of both squamous cell carcinoma and adenocarcinoma. 展开更多
关键词 lung cancer adenosquamous carcinoma
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Enhanced chemosensitivity of p73α gene transferred into H1299 cell line of human lung adenocarcinoma 被引量:2
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作者 何勇 范士志 +2 位作者 Kalkunte S Srivenugopal 蒋耀光 秦川 《Journal of Medical Colleges of PLA(China)》 CAS 2004年第3期151-156,共6页
Objective: To study the effects of transferred wild type p73α gene on the sensitivity to the chemotherapeutic agents and the growth of p53-null H1299 cells of human lung adenocarcinoma. Methods: The pcDNA3-HA-p73α p... Objective: To study the effects of transferred wild type p73α gene on the sensitivity to the chemotherapeutic agents and the growth of p53-null H1299 cells of human lung adenocarcinoma. Methods: The pcDNA3-HA-p73α plasmid was transferred into the cultured p53-null H1299 cells of human lung adenocarcinoma with the mediation of Dosper liposome; The cells resistant to G418 were selected. The expression of p73α gene in the cells was examined with Western blot. MTT assay was used to analyze the response of the transfected cells to cis-dichlorodiamine platinum (cDDP) and adriamycin (ADM). The rate of drug-induced apoptosis of the transfected cells was determined with flow cytometry and DNA fragmentation assay. The changes of the biological behaviors were observed with colony formation assay. Results: The transfected H1299 cells of human lung adenocarcinoma over-expressed p73α protein stably. MTT assay showed that the IC 50 values of cDDP and ADM were reduced by approximately 7 fold and 130 fold respectively in the transfected cells as compared with the untransfected ones. Lower concentration of the chemotherapeutic agents (1.25 μmol/L of cDDP and 0.05 μmol/L of ADM) could be employed to suppress markedly the growth of the transfected H1299 cells. The apoptotic rate induced by cDDP was increased from 10.1% to 38 4% (P<0.01) and that of ADM from 12.1% to 49.3% (P<0.01). The clonogenecity after the administration of chemotherapeutic agents was significantly lower in the transfected H1299 cells than in the parental cells (P<0.01). The sensitive enhancement ratios were 1.8 and 2.6 for cDDP and ADM respectively. Conclusion: The transfection of H1299 cells with wild type p73α gene results in an increase of the sensitivity of the cells to chemotherapeutic agents. 展开更多
关键词 p73α gene lung adenocarcinoma gene therapy CHEMOSENSITIVITY
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Application of video-assisted thoracic surgery in the standard operation for thoracic tumors 被引量:9
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作者 Ju-Wei Mu Gui-Yu Chen +22 位作者 Ke-Lin Sun Da-Wei Wang Bai-Hua Zhang Ning Li Fang Lv You-Sheng Mao Qi Xue Shu-Geng Gao Jun Zhao Da-Li Wang Zhi-Shan Li Wen-Dong Lei Yu-Shun Gao Liangze Zhang Jin-Feng Huang Kang Shao Kai Su Kun Yang Liang Zhao Fei-Yue Feng Yong-Gang Wang Jian Li Jie He 《Cancer Biology & Medicine》 SCIE CAS CSCD 2013年第1期28-35,共8页
Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed. These patients underwent... Objective: To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors. Methods: The data of 1,790 consecutive patients were retrospectively reviewed. These patients underwent VATS pulmonary resections, VATS esophagectomies, and VATS resections of mediastinal tumors or biopsies at the Cancer Institute & Hospital, Chinese Academy of Medical Sciences between January 2009 and January 2012. Results: There were 33 patients converted to open thoracotomy (OT, 1.84%). The overall morbidity and mortality rate was 2.79% (50/1790) and 0.28% (5/1790), respectively. The overall hospitalization and chest tube duration were shorter in the VATS lobectomy group (n=949) than in the open thoracotomy (OT) lobectomy group (n=753). There were no significant differences in morbidity rate, mortality rate and operation time between the two groups. In the esophageal cancer patients, no significant difference was found in the number of nodal dissection, chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS esophagectomy group (n=8 1) and open esophagectomy group (n=81). However, the operation time was longer in the VATS esophagectomy group. In the thymoma patients, there was no significant difference in the chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS thymectomy group (n=41) and open thymectomy group (n=41). However, the operation time was longer in the VATS group. The median tumor size in the VATS thymectomy group was comparable with that in the OT group. Conclusions: In early-stage (Ⅰ/Ⅱ) non-small cell lung cancer patients who underwent lobectomies, VATS is comparable with the OT approach with similar short-term outcomes. In patients with resectable esophageal cancer, VATS esophagectomy is comparable with OT esophagectomy with similar morbidity and mortality. VATS thymectomy for Masaoka stage I and II thymoma is feasible and safe, and tumor size is not contraindicated. Longer follow-ups are needed to determine the oncologic equivalency of VATS lobectomy, esophagectomy, and thymectomy for thymoma vs. OT. 展开更多
关键词 Video-assisted thoracic surgery (VATS) non-small cell lung cancer (NSCLC) esophageal cancer THYMOMA
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Empirical studies about quercetin increasing chemosensitivity on human lung adenocarcinoma cell line A549 被引量:1
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作者 Xuejun Zhan Runxiang Zhang +3 位作者 Yanping Xu Shuhua Yang Daze Xie Liwei Tan 《The Chinese-German Journal of Clinical Oncology》 CAS 2012年第7期380-383,共4页
Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) ... Objective: The present study was designed to investigate whether quercetin exerts increasing chemosensitivity on human lung adenocarcinoma cells when quercetin combined with cisplatin (DDP) and vincristine (VCR) in vitro respectively and its possible antitumor mechanism. To provide experimental proof for clinical combination application. Methods: Using intermittent administration of high dose VCR, human lung adenocarcinoma sensitive cell line (A549/S) was induced to VCR- resistant human lung adenocarcinoma cell line (A549NCR). MTT assay was adapted for examing the 50% inhibition (IC50) value of DDP and VCR on A549/S and A549/VCR when quercetin combined with DDP and VCR respectively. Results: IC50 of DDP on A549/S and A549/VCR was 10.18 and 12.35 mg/L, and the IC50 of VCR on the two cell lines was 1.21 and 12.77 rag/L, respectively. The resistance fold of A549/VCR on VCR and DDP was 10.55 and 121, respectively. When quercetin at concentration of 50, 100 and 200 pmol/L in combination with DDP and VCR respectively, the IC50 of DDP and VCR on A549/S and A549/VCR were obvious decreased (P 〈 0.05 - P 〈 0.01). Conclusion: The experiment results suggested that quercetin could increase the chemosensitivity and partly revise the resistance of A549NCR. 展开更多
关键词 quercetin (Que) A549 lung adenocarcinoma cell line cisplatin (DDP) vincristine (VCR) increase chemosen-sitivity
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Prognostic value of site-specific metastases in pancreatic adenocarcinoma: A Surveillance Epidemiology and End Results database analysis 被引量:14
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作者 Hani Oweira Ulf Petrausch +7 位作者 Daniel Helbling Jan Schmidt Meinrad Mannhart Arianeb Mehrabi Othmar Schob Anwar Giryes Michael Decker Omar Abdel-Rahman 《World Journal of Gastroenterology》 SCIE CAS 2017年第10期1872-1880,共9页
To evaluate the prognostic value of site-specific metastases among patients with metastatic pancreatic carcinoma registered within the Surveillance, Epidemiology and End Results (SEER) database.METHODSSEER database (2... To evaluate the prognostic value of site-specific metastases among patients with metastatic pancreatic carcinoma registered within the Surveillance, Epidemiology and End Results (SEER) database.METHODSSEER database (2010-2013) has been queried through SEER*Stat program to determine the presentation, treatment outcomes and prognostic outcomes of metastatic pancreatic adenocarcinoma according to the site of metastasis. In this study, metastatic pancreatic adenocarcinoma patients were classified according to the site of metastases (liver, lung, bone, brain and distant lymph nodes). We utilized chi-square test to compare the clinicopathological characteristics among different sites of metastases. We used Kaplan-Meier analysis and log-rank testing for survival comparisons. We employed Cox proportional model to perform multivariate analyses of the patient population; and accordingly hazard ratios with corresponding 95%CI were generated. Statistical significance was considered if a two-tailed P value < 0.05 was achieved.RESULTSA total of 13233 patients with stage IV pancreatic cancer and known sites of distant metastases were identified in the period from 2010-2013 and they were included into the current analysis. Patients with isolated distant nodal involvement or lung metastases have better overall and pancreatic cancer-specific survival compared to patients with isolated liver metastases (for overall survival: lung vs liver metastases: P < 0.0001; distant nodal vs liver metastases: P < 0.0001) (for pancreatic cancer-specific survival: lung vs liver metastases: P < 0.0001; distant nodal vs liver metastases: P < 0.0001). Multivariate analysis revealed that age < 65 years, white race, being married, female gender; surgery to the primary tumor and surgery to the metastatic disease were associated with better overall survival and pancreatic cancer-specific survival.CONCLUSIONPancreatic adenocarcinoma patients with isolated liver metastases have worse outcomes compared to patients with isolated lung or distant nodal metastases. Further research is needed to identify the highly selected subset of patients who may benefit from local treatment of the primary tumor and/or metastatic disease. 展开更多
关键词 Pancreatic cancer Liver metastases Lung metastases Bone metastases Surveillance Epidemiology and End Results database
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Clinical Observation on Treatment of NonParvicellular Carcinoma of the Lung with Jin Fu Kang Oral Liquid 被引量:1
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作者 刘嘉湘 施志明 +6 位作者 徐振晔 朱晏伟 赵丽红 李和根 高虹 陈善香 刘煜 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2000年第2期96-100,共5页
Jin Fu Kang Oral Liquid ([symbol: see text]), made of traditional Chinese drugs for supplementing qi and nourishing yin, was developed according to the common symptoms in lung carcinoma with deficiency of both qi and ... Jin Fu Kang Oral Liquid ([symbol: see text]), made of traditional Chinese drugs for supplementing qi and nourishing yin, was developed according to the common symptoms in lung carcinoma with deficiency of both qi and yin. Of the 96 cases in the Jin Fu Kang group, 1 case got complete remission (CR) after treatment, 8 cases partial remission (PR), 52 cases no change (NC), PR + NC covering 63.5%. Of the 52 cases in the group of Jin Fu Kang plus chemotherapy, 11 cases got PR after treatment, 26 cases NC, PR + NC covering 71.2%. Of the 25 cases in the chemotherapy group, 4 cases got PR after treatment, 11 cases NC, PR + NC covering 60.0%. The results show that the therapeutic effectiveness in the Jin Fu Kang group and the group of Jin Fu Kang plus chemotherapy was better than that in the chemotherapy group. The one-year survival rate and the two-year survival rate after treatment in the Jin Fu Kang group were 67.3% and 67.3% respectively; 66.7% and 66.7% in the group of Jin Fu Kang plus chemotherapy; and 40.3% and 0.0% in the chemotherapy group. The improvement of clinical symptoms, increase of body weight and improvement of health situation (KPS marks) after treatment in both the Jin Fu Kang group and the group of Jin Fu Kang plus chemotherapy were better than that in the chemotherapy group. Some indicators of immunology and hemogram after treatment were greatly improved in the Jin Fu Kang group, worse in the chemotherapy group, but no obvious improvement in the group of Jin Fu Kang plus chemotherapy. 展开更多
关键词 ADENOCARCINOMA Carcinoma Non-Small-Cell Lung Carcinoma Squamous Cell Drugs Chinese Herbal Humans Lung Neoplasms
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Associations Between Epidermal Growth Factor Receptor Gene Mutation and Serum Tumor Markers in Advanced Lung Adenocarcinomas: A Retrospective Study 被引量:2
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作者 Ying-qiu Pan Wei-wu Shi +3 位作者 Dan-ping Xu Hui-hui Xu Mei-ying Zhou Wei-hua Yan 《Chinese Medical Sciences Journal》 CAS CSCD 2014年第3期156-161,共6页
Objective To investigate the associations between epidermal growth factor receptor (EGFR) gene mutations and serum tumor markers in advanced lung adenocarcinomas. Methods We investigated the association between EGF... Objective To investigate the associations between epidermal growth factor receptor (EGFR) gene mutations and serum tumor markers in advanced lung adenocarcinomas. Methods We investigated the association between EGFR gene mutations and clinical features, including serum tumor marker levels, in 97 advanced lung adenocarcinomas patients who did not undergo the treatment of EGlaR tyrosine kinase inhibitors. EGFR gene mutation was detected by real-time PCR at exons 18, 19, 20, and 21. Serum tumor marker concentrations were analyzed by chemiluminescence assay kit at the same time. Results EGFR gene mutations were detected in 42 (43%) advanced lung adenocarcinoma patients. Gender (P=0.003), smoking status (P=0.001), and abnormal serum status of carcinoembryonic antigen (CEA, P=0.028) were significantly associated with EGFR gene mutation incidence. Multivariate analysis showed the abnormal CEA level in serum was independently associated with the incidence of EGFR gene mutation (P=0.046) with an odds ratio of 2.613 (95% Ch 1.018-6.710). However, receiver operating characteristic (ROC) curve analysis revealed CEA was not an ideal predictive marker for EGFR gene mutation status in advanced lung adenocarcinoma (the area under the ROC curve was 0.608, P=0.069). Conclusions EGFR gene mutation status is significantly associated with serum CEA status in advanced lung adenocarcinmoas. However, serum CEA is not an ideal predictor for EGFR mutation. 展开更多
关键词 advanced lung adenocarcinomas epidermal growth factor receptor gene MUTATION epidermal growth factor receptor tyrosine kinase inhibitor carcinoembryonic antigen
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Phase Ⅲ Clinical Trials of the Cell Differentiation Agent-2 (CDA-2): Therapeutic Efficacy on Breast Cancer, Non-Small Cell Lung Cancer and Primary Hepatoma 被引量:3
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作者 Fengyi Feng Qing Lu +24 位作者 Changquan Ling Yang Zhang Fengzhan Qin Huaqing Wang Wenxia Huang Shunchang Jiao Qiang Chen Mingzhong Li Yunzhong Zhu Meizhen Zhou Jun Ren Yetao Gao Jingpo Zhao Rongsheng Zheng Wenhua Zhao Zhiqiang Meng Fang Li Qizhong Zhang Dongli Zhao Liyan Xu Yongqiang Zhang Yanjun Zhang Zhenjiu Wang Shuonqi Liu Ming C. Liau 《Chinese Journal of Clinical Oncology》 CSCD 2005年第4期706-716,共11页
OBJECTIVE The objective of this study was to explore the effect of CDA-2, a selective inhibitor of abnormal methylation enzymes in cancer cells, on the therapeutic efficacy of cytotoxic chemotherapy. METHODS Advanced ... OBJECTIVE The objective of this study was to explore the effect of CDA-2, a selective inhibitor of abnormal methylation enzymes in cancer cells, on the therapeutic efficacy of cytotoxic chemotherapy. METHODS Advanced cancer patients, all of whom had previously undergone chemotherapy, were randomly divided into 2 groups, one receiving chemotherapy only as the control group, and the other receiving CDA-2 in addition to chemotherapy as the combination group. The therapeutic efficacies and the toxic maniestations of the 2 groups were compared based on the WHO criteria. RESULTS Of 454 cancer patients enrolled in phase Ⅲ clinical trials of CDA-2, 80, 188, and 186 were breast cancer, NSCLC, and primary hepatoma patients, respectively. Among them 378 patients completed treatments according to the protocols. The results showed that the overall effective rate of the combination group was 2.6 fold that of the control group, 4.8 fold in the case of breast cancer, 2.3 fold in the case of primary hepatoma, and 2.2 fold in the case of NSCLC. Surprisingly, the combination therapy appeared to work better for stage Ⅳ than stage Ⅲ patients. CDA-2 did not contribute additional toxicity. On the contrary, it reduced toxic manifestations of chemotherapy, particularly regarding white blood cells, nausea and vomiting. CONCLUSION Modulation of abnormal methylation enzymes by CDA-2 is definitely helpful to supplement chemotherapy. It significantly increased the therapeutic efficacy and reduced the toxic manifestation of cytotoxic chemotherapy on breast cancer and NSCLC. 展开更多
关键词 abnormal methylation enzymes DNA hypomethylation differentiation therapy adjuvant chemotherapy
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A complicated case of lung cancer and pulmonary tuberculosis initially presented with painful scapular metastasis in other side 被引量:1
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作者 Hassan-Ali Mohebbi Amin Saburi +1 位作者 Mohammad-Hasan Bagheri Ehsan Saburi 《The Chinese-German Journal of Clinical Oncology》 CAS 2012年第5期294-296,共3页
We report a 43-year-old man who presented with a right painful shoulder mass and bilateral lung masses in computed tomography(CT).Scapular mass was excised and pathology report demonstrated high-grade metastatic tumor... We report a 43-year-old man who presented with a right painful shoulder mass and bilateral lung masses in computed tomography(CT).Scapular mass was excised and pathology report demonstrated high-grade metastatic tumor.Same side lung biopsy and histopathological study characterized tuberculosis but biopsy of the left lung lesion identified adenocarcinoma of the lung.The final diagnosis was right scapular metastatic lesion from left lung adenocarcinoma.Musculoskeletal symptoms are commonly encountered in lung malignancies due to paraneoplastic syndrome or hematogenous metastasis but scapular metastasis on the other side as the presentation of lung cancer is extremely rare. 展开更多
关键词 lung cancer (LC) SCAPULA tuberculosis METASTASIS ADENOCARCINOMA
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