The effects of La 3+ on outward K+ channels at plasma membrane in vicia mesophyll cells were studied by using the whole-cell patch-clamp recording mode. It was shown that La 3+ on both sides of plasma membrane blocked...The effects of La 3+ on outward K+ channels at plasma membrane in vicia mesophyll cells were studied by using the whole-cell patch-clamp recording mode. It was shown that La 3+ on both sides of plasma membrane blocked outward K+ currents in a concentration-dependent manner. The dose response was fitted by the Michalis- Menten relationship characterized by an inhibitory constant K i of (76.59±9.28) μmol/L(outside membrane) and (5.80±0.62)×10 -15 mol/L(inside membrane) respectively, suggesting that intra-cellular La 3+ had a stronger inhibitory effect on outward K+ currents than extra-cellular La 3+ did. Since K+ remains the dominant osmoticum in mesophyll cells, the inhibition of outward K+ currents by La 3+ may contribute greatly to preventing the K+ loss and promoting turgor maintenance. These results implied that rare earth elements might have potential practical values in regulating plant water status and strengthening plant drought endurance.展开更多
目的研究Long Evans大鼠不同发育阶段视网膜神经节细胞(Retinal Ganglion Cells, RGCs)电生理学特性,认识视觉发育过程中视网膜神经元成熟的细胞内机制.方法取出生后3～31 d Long Evans大鼠,制备视网膜片,寻找RGCs行膜片钳全细胞记录,...目的研究Long Evans大鼠不同发育阶段视网膜神经节细胞(Retinal Ganglion Cells, RGCs)电生理学特性,认识视觉发育过程中视网膜神经元成熟的细胞内机制.方法取出生后3～31 d Long Evans大鼠,制备视网膜片,寻找RGCs行膜片钳全细胞记录,给予不同强度去极化脉冲,诱发动作电位.结果 33只Long Evans大鼠共获得94个RGCs,按动作电位类型RGCs分为单锋型、瞬变型和持续型,在视觉发育不同阶段,3种类型的RGCs所占比例不同,在电生理特性上有显著差异.结论在视觉发育过程中,RGCs的电学特性逐渐成熟,动作电位的幅度、频率存在差异,提示不同类型的RGCs在编码及传递时间、空间视觉信息中具有不同作用.展开更多
文摘The effects of La 3+ on outward K+ channels at plasma membrane in vicia mesophyll cells were studied by using the whole-cell patch-clamp recording mode. It was shown that La 3+ on both sides of plasma membrane blocked outward K+ currents in a concentration-dependent manner. The dose response was fitted by the Michalis- Menten relationship characterized by an inhibitory constant K i of (76.59±9.28) μmol/L(outside membrane) and (5.80±0.62)×10 -15 mol/L(inside membrane) respectively, suggesting that intra-cellular La 3+ had a stronger inhibitory effect on outward K+ currents than extra-cellular La 3+ did. Since K+ remains the dominant osmoticum in mesophyll cells, the inhibition of outward K+ currents by La 3+ may contribute greatly to preventing the K+ loss and promoting turgor maintenance. These results implied that rare earth elements might have potential practical values in regulating plant water status and strengthening plant drought endurance.
文摘目的研究Long Evans大鼠不同发育阶段视网膜神经节细胞(Retinal Ganglion Cells, RGCs)电生理学特性,认识视觉发育过程中视网膜神经元成熟的细胞内机制.方法取出生后3～31 d Long Evans大鼠,制备视网膜片,寻找RGCs行膜片钳全细胞记录,给予不同强度去极化脉冲,诱发动作电位.结果 33只Long Evans大鼠共获得94个RGCs,按动作电位类型RGCs分为单锋型、瞬变型和持续型,在视觉发育不同阶段,3种类型的RGCs所占比例不同,在电生理特性上有显著差异.结论在视觉发育过程中,RGCs的电学特性逐渐成熟,动作电位的幅度、频率存在差异,提示不同类型的RGCs在编码及传递时间、空间视觉信息中具有不同作用.
