Mercaptobenzothiozol self-assembled monolayer modified electrode was fabricated successfully,and cylic voltammetry and scanning electronic microscopy were used to characterize the SAMs modified electrode.The electroch...Mercaptobenzothiozol self-assembled monolayer modified electrode was fabricated successfully,and cylic voltammetry and scanning electronic microscopy were used to characterize the SAMs modified electrode.The electrochemical behaviors of emtol and P-benzenediol were studied on the SAMs in Britton-Robinson buffer solution.The results of the experiments indicate that metol and p-benzenediol can be catalyzed on the SAMs.They can not only be determined singleness but also be determined in mixture simultaneously and doesn’t interfere with each other in a large range of concentration.This method has been used to determine metol and P-benzenediol in waste photographic imaging agent with satisfactory results.展开更多
A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-et...A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.展开更多
文摘Mercaptobenzothiozol self-assembled monolayer modified electrode was fabricated successfully,and cylic voltammetry and scanning electronic microscopy were used to characterize the SAMs modified electrode.The electrochemical behaviors of emtol and P-benzenediol were studied on the SAMs in Britton-Robinson buffer solution.The results of the experiments indicate that metol and p-benzenediol can be catalyzed on the SAMs.They can not only be determined singleness but also be determined in mixture simultaneously and doesn’t interfere with each other in a large range of concentration.This method has been used to determine metol and P-benzenediol in waste photographic imaging agent with satisfactory results.
文摘A NOS gene electrochemical biosensor was prepared by covalently immobilizing of NOS ssDNA sequence on the mercaptoacetic acid monolayer self-assembled gold electrode with the help of N-（3-dimethylaminopropyl）-N’-ethylcarbodiimide hydrochloride（EDC）and N-hydroxysuccinimide（NHS）.By using methylene blue as the electrochemical indicator,the increase of cathodic peak current was in linear with the concentration of complementary ssDNA in the concentration range of 5.0×10-8 1.0×10-4 mol/L with the linear regression equation as ΔI pc=1.10lgc+8.25（n=8,γ=0.998 5）and the detection limit as 3.60×10-8 mol/L（3σ）.
