The mycotoxin, patulin （4-hydroxy-4H-furo [3, 2c] pyran-2 [6H]-one）, is produced by a number of fungi common to fruit and vegetable-based products, most notably apples. Patulin contamination within apple products po...The mycotoxin, patulin （4-hydroxy-4H-furo [3, 2c] pyran-2 [6H]-one）, is produced by a number of fungi common to fruit and vegetable-based products, most notably apples. Patulin contamination within apple products poses a serious health risk to consumers. Studies done on laboratory animals have demonstrated that patulin has a broad spectrum of toxicity, including mutagen city and carcinogenicity. The aim of the experiment was studying influence of selectively acting activated carbon powder--Ercarbon SH （Erbsloh, Germany） which is special produced for lowering HMF （hydroxy methyl furfural）, on reduction of patulin content in clear apple juice. Industrial apple row material with some damaged parts was pressed, juice was pasteurized at 95 ℃ during 2 min. After cooling on 55 ℃, enzymatic treated and clarified juice were filtered by 0.45 [am pore sizes membrane filter, Apple clear juice sample was divided for five parts. The samples of apple juice were diluted to 11.5° Brix and contacted with concentrations of 2, 2.5, 3 and 3.5 g/L activated carbon powder for 30 min. After filtration in the experimental samples, putulin was quantitatively determined by HPLC （high performance liquid chromatography with UV） detector at 276 nm. The research revealed that the best results were achieved by treatment with activated carbon in its powder form at concentration of 2.5 g/L with 30 min contact time.展开更多
Seperation and purifitation of human chorionic gonadotropion (HCG) in theurine sample of early pregnant women by D3520 resin adsorption chromatographyis repaied. The crude product obtained by DEAE - Cellulose 23 and ...Seperation and purifitation of human chorionic gonadotropion (HCG) in theurine sample of early pregnant women by D3520 resin adsorption chromatographyis repaied. The crude product obtained by DEAE - Cellulose 23 and DEAE Sephadex A50 column chromatography showed a high activity of HCG. Furtherpurifikation of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000～ 6500 IU/mg. The preparation meets therequiTements of the pyrogn test in Chinese Law of Pharmacopoeia.展开更多
Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discove...Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discovery of new drugs. However, TCMs usually contain hundreds or even thousands of components and only a few of these compounds are responsible for the pharmacological effects. The large number of components in TCMs makes the analysis and screening for bioactive compounds extremely difficult. Therefore, strategies for analysis and screening of bioactive compounds in TCMs have long been a focus of studies on TCMs. Cell membrane chromatography （CMC） has become a useful tool for studying the bioactive compounds of TCMs. This review summarizes the principles, characteristics, and application of CMC, and the possible problems faced when working with TCMs. This method will likely become increasingly important for screening and analysis of hioactive compounds in TCMs.展开更多
文摘The mycotoxin, patulin （4-hydroxy-4H-furo [3, 2c] pyran-2 [6H]-one）, is produced by a number of fungi common to fruit and vegetable-based products, most notably apples. Patulin contamination within apple products poses a serious health risk to consumers. Studies done on laboratory animals have demonstrated that patulin has a broad spectrum of toxicity, including mutagen city and carcinogenicity. The aim of the experiment was studying influence of selectively acting activated carbon powder--Ercarbon SH （Erbsloh, Germany） which is special produced for lowering HMF （hydroxy methyl furfural）, on reduction of patulin content in clear apple juice. Industrial apple row material with some damaged parts was pressed, juice was pasteurized at 95 ℃ during 2 min. After cooling on 55 ℃, enzymatic treated and clarified juice were filtered by 0.45 [am pore sizes membrane filter, Apple clear juice sample was divided for five parts. The samples of apple juice were diluted to 11.5° Brix and contacted with concentrations of 2, 2.5, 3 and 3.5 g/L activated carbon powder for 30 min. After filtration in the experimental samples, putulin was quantitatively determined by HPLC （high performance liquid chromatography with UV） detector at 276 nm. The research revealed that the best results were achieved by treatment with activated carbon in its powder form at concentration of 2.5 g/L with 30 min contact time.
文摘Seperation and purifitation of human chorionic gonadotropion (HCG) in theurine sample of early pregnant women by D3520 resin adsorption chromatographyis repaied. The crude product obtained by DEAE - Cellulose 23 and DEAE Sephadex A50 column chromatography showed a high activity of HCG. Furtherpurifikation of the sample by gel filtration chromatography on a Sephadex G75 column gives a final preparation of 6000～ 6500 IU/mg. The preparation meets therequiTements of the pyrogn test in Chinese Law of Pharmacopoeia.
基金Scientific and Technological Innovation Project Foundation of Shanxi China (Grant No. 20090321099)
文摘Traditional Chinese medicines （TCMs） have a long history of clinical testing and reliable therapeutic efficacy. They have attracted international attention as excellent sources of bioactive compounds for the discovery of new drugs. However, TCMs usually contain hundreds or even thousands of components and only a few of these compounds are responsible for the pharmacological effects. The large number of components in TCMs makes the analysis and screening for bioactive compounds extremely difficult. Therefore, strategies for analysis and screening of bioactive compounds in TCMs have long been a focus of studies on TCMs. Cell membrane chromatography （CMC） has become a useful tool for studying the bioactive compounds of TCMs. This review summarizes the principles, characteristics, and application of CMC, and the possible problems faced when working with TCMs. This method will likely become increasingly important for screening and analysis of hioactive compounds in TCMs.
