Members of the Rho family of GTPases are key regulators of the actin cytoskeleton. In particular, activated Racl stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor （PDGF）. Abl-...Members of the Rho family of GTPases are key regulators of the actin cytoskeleton. In particular, activated Racl stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor （PDGF）. Abl-interactor （Abi）- 1 and βP1X, a guanine nucleotide exchange factor for Racl, localise at these Rac1-induced actin structures and play important roles in the induction of membrane dorsal ruffling in response to PDGF in fibroblasts. Here, we demonstrate a novel interaction between Abi-1 and βPIX using the yeast two-hybrid system, in vitro pull-down assays, and in vivo co-immunoprecipitation experiments. In vitro, the C-terminal fragment of βPIX interacted with Abi-1, while in vivo the N-terminal fragment of βPIX interacted with Abi-1. The biological function of this interaction was investigated in mouse fibroblasts in response to PDGF stimulation. Abi-1 and βPIX co-localised in the cytoplasm and to membrane dorsal ruffles after PDGF treatment. We show that the co-expression of Abi-1 and truncated forms of βPIX in mouse fibroblasts blocked PDGF-induced membrane dorsal ruffles. Together, these results show that the interaction between Abi-1 and βPIX is involved in the formation of growth factor-induced membrane dorsal ruffles.展开更多
Metal-organic framework(MOF) thin films are multilayer materials ranging from nanometers to micrometers in thickness,physically or chemically adhesive to a(functionalized) substrate and,in an ideal case,exhibiting low...Metal-organic framework(MOF) thin films are multilayer materials ranging from nanometers to micrometers in thickness,physically or chemically adhesive to a(functionalized) substrate and,in an ideal case,exhibiting low roughness and high homogeneity.Various innovative approaches have been developed for MOF thin film fabrication.Among these advanced materials,surface-attached metal-organic frameworks(SURMOFs) are an important class of MOF films.SURMOFs,fabricated in a step-by-step liquid phase epitaxial(LPE) fashion by alternating deposition of metal and organic linker precursors on a functionalized substrate,for example,thiolate-based self-assembled monolayers(SAMs),have already exhibited their utility in both research and potential applications.SURMOFs combine surface science and the chemistry of MOFs,possessing the following unique advantages that cannot be accessed through other methods:(i) precisely controlling thickness,roughness and homogeneity as well as growth orientation,(ii) studying of MOF growth mechanism,(iii) modifying/tailoring MOFs' structures during the SURMOF growth and thus creating customizable properties,and(iv) existing in the form of thin film/membrane for direct applications,for example,as sensors.This review discusses the oriented and crystalline SURMOFs fabricated by LPE approach,covering their preparation,growth mechanism,and characterization methodology as well as applications based upon the most newly updated knowledge.展开更多
Continuous copper net-supported metal-organic framework (MOF-5) membranes of high quality are synthesized for the first time by vapor diffusion of an organic amine. The velocity of diffusion of the organic amine and r...Continuous copper net-supported metal-organic framework (MOF-5) membranes of high quality are synthesized for the first time by vapor diffusion of an organic amine. The velocity of diffusion of the organic amine and reaction temperature directly affect the quality of the membranes formed. Furthermore, a laser dye is successfully encapsulated within such membranes in CHCl3. The dye-loaded MOF-5 membranes have potential applications in optics, especially in laser systems.展开更多
文摘Members of the Rho family of GTPases are key regulators of the actin cytoskeleton. In particular, activated Racl stimulates membrane dorsal ruffle formation in response to platelet-derived growth factor （PDGF）. Abl-interactor （Abi）- 1 and βP1X, a guanine nucleotide exchange factor for Racl, localise at these Rac1-induced actin structures and play important roles in the induction of membrane dorsal ruffling in response to PDGF in fibroblasts. Here, we demonstrate a novel interaction between Abi-1 and βPIX using the yeast two-hybrid system, in vitro pull-down assays, and in vivo co-immunoprecipitation experiments. In vitro, the C-terminal fragment of βPIX interacted with Abi-1, while in vivo the N-terminal fragment of βPIX interacted with Abi-1. The biological function of this interaction was investigated in mouse fibroblasts in response to PDGF stimulation. Abi-1 and βPIX co-localised in the cytoplasm and to membrane dorsal ruffles after PDGF treatment. We show that the co-expression of Abi-1 and truncated forms of βPIX in mouse fibroblasts blocked PDGF-induced membrane dorsal ruffles. Together, these results show that the interaction between Abi-1 and βPIX is involved in the formation of growth factor-induced membrane dorsal ruffles.
基金the European Union for funding the research projects on MOF thin films (Priority Program 1362 of the DFG)SUR-MOFs(6th FP, NMP4-CT-2006-032109)
文摘Metal-organic framework(MOF) thin films are multilayer materials ranging from nanometers to micrometers in thickness,physically or chemically adhesive to a(functionalized) substrate and,in an ideal case,exhibiting low roughness and high homogeneity.Various innovative approaches have been developed for MOF thin film fabrication.Among these advanced materials,surface-attached metal-organic frameworks(SURMOFs) are an important class of MOF films.SURMOFs,fabricated in a step-by-step liquid phase epitaxial(LPE) fashion by alternating deposition of metal and organic linker precursors on a functionalized substrate,for example,thiolate-based self-assembled monolayers(SAMs),have already exhibited their utility in both research and potential applications.SURMOFs combine surface science and the chemistry of MOFs,possessing the following unique advantages that cannot be accessed through other methods:(i) precisely controlling thickness,roughness and homogeneity as well as growth orientation,(ii) studying of MOF growth mechanism,(iii) modifying/tailoring MOFs' structures during the SURMOF growth and thus creating customizable properties,and(iv) existing in the form of thin film/membrane for direct applications,for example,as sensors.This review discusses the oriented and crystalline SURMOFs fabricated by LPE approach,covering their preparation,growth mechanism,and characterization methodology as well as applications based upon the most newly updated knowledge.
基金supported by the Department of Education Science and Technology Project of Heilongjiang (11551547)
文摘Continuous copper net-supported metal-organic framework (MOF-5) membranes of high quality are synthesized for the first time by vapor diffusion of an organic amine. The velocity of diffusion of the organic amine and reaction temperature directly affect the quality of the membranes formed. Furthermore, a laser dye is successfully encapsulated within such membranes in CHCl3. The dye-loaded MOF-5 membranes have potential applications in optics, especially in laser systems.
