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噬菌体Bp7耐受株K12-R生物学特性分析
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作者 陈培培 齐心 +3 位作者 王薇 任慧英 刘文华 张灿 《畜牧兽医学报》 CAS CSCD 北大核心 2018年第2期396-400,共5页
宿主菌E.coli K12的突变株K12-R不能被噬菌体Bp7裂解,为了明确其产生耐受的原因以及突变对其生物学性能的影响,对K12-R进行全基因组测序,分析其产生耐受的原因,浊度测定K12-R的生长曲线和自凝能力,革兰染色检测K12-R生物膜的形成能力,比... 宿主菌E.coli K12的突变株K12-R不能被噬菌体Bp7裂解,为了明确其产生耐受的原因以及突变对其生物学性能的影响,对K12-R进行全基因组测序,分析其产生耐受的原因,浊度测定K12-R的生长曲线和自凝能力,革兰染色检测K12-R生物膜的形成能力,比较K12-R和E.coli K12之间生物学性能的变化。结果表明,突变株K12-R脂多糖合成相关基因hldE发生了突变,突变株K12-R细胞膜通透性增加,生长繁殖能力降低,自凝能力增强,生物膜形成能力增强。E.coli K12的脂多糖合成基因hldE突变能够改变K12-R脂多糖的结构,从而抑制噬菌体Bp7的吸附。这为明确K12-R突变株对噬菌体Bp7的耐受机制研究提供了理论依据。 展开更多
关键词 大肠杆菌K12 K12-R 生物性能 自凝能力 生物膜
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Fatigue properties of special kind of reinforced concrete composite beams
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作者 胡铁明 黄承逵 陈小锋 《Journal of Central South University》 SCIE EI CAS 2010年第1期142-149,共8页
The special reinforced concrete composite beam consists of a steel fiber reinforced self-stressing concrete composite layer and a reinforced concrete T-beam, and constructional bars are set up at their bonding interfa... The special reinforced concrete composite beam consists of a steel fiber reinforced self-stressing concrete composite layer and a reinforced concrete T-beam, and constructional bars are set up at their bonding interface. Fatigue properties of the composite beam under the action of negative moment were experimentally studied. Through inverted loading mode the load-beating state of a composite beam was simulated under the action of negative moment. With the ratios of constructional bars being 0, 0.082% and 0.164% respectively as parameters, the effects of constructional bars on the properties of composite beam, such as fatigue life, crack propagation, rigidity loss as well as damage behavior of bonding interface, were studied. The mechanism of the constructional bars on the fatigue properties of the composite beams and the restriction mechanism of crack widths and rigidity loss were analyzed. The test results show that the constructional bars can enhance the shear resistance of the bonding interface between composite layer and old concrete beam and restrict expanding of steel fiber reinforced self-stressing concrete, which are beneficial to synergistic action of composite layer and old concrete beam, to reducing the stress amplitude of bars and the crack width of composite layer, and to increasing the durability and fatigue life of the composite beam. 展开更多
关键词 steel fiber reinforced self-stressing concrete composite beam constructional bar bonding interface FATIGUE
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