自分化法培养大鼠骨髓间充质干细胞移植治疗大鼠帕金森病的研究

目的观察自分化法培养的大鼠骨髓间充质干细胞(SDrBMSCs)向大鼠纹状体内移植大鼠帕金森病(PD)模型的治疗作用。方法 SDrBMSCs以不换液、不外加任何诱导物质培养,用免疫细胞化学染色鉴定SDrBMSCs及用普通法培养大鼠骨髓间充质干细胞(rBMSCs)的分化情况,制作大鼠PD模型,设SDrBMSCs移植组、rBMSCs移植组(PD大鼠12只/组),向PD大鼠纹状体内植入SDrBMSCs或rBMSCs(1×106个/只),以免疫荧光组织化学染色鉴定其在纹状体部细胞体内存活迁移能力,高效液相色谱电化学方法(HPLC)检测纹状体内多巴胺(DA)含量,同时设PBS模型对照组(PD大鼠12只/组)做比较。结果 SDrBMSCs培养至第4代21 d移植入PD大鼠纹状体内,在无外源干预的情况下,表达nestin、β-tublinⅢ、DCX和MAP2,而同样条件移植入rBMSCs的PD大鼠仅表达少量的nestin;SDrBMSCs或rBMSCs移植入大鼠纹状体内后均可在体内存活、迁移并与周围组织整合。SDrBMSCs移植组PD大鼠在移植后28 d能表达GFAP,并有分化为TH阳性神经元的现象,使纹状体内DA含量提高,但与移植后14 d比较无明显变化(P>0.05);PD大鼠的旋转行为以移植后56 d时改善最为明显(P<0.05),旋转降至移植前的55.02%。rBMSCs移植组PD大鼠在移植后28 d可表达GFAP,也有改善PD大鼠旋转行为的效果(P<0.05),但纹状体内DA含量明显低于植入SDrBMSCs的效果(P<0.05)。结论 SDrBMSCs脑内移植后主要分化为星形胶质细胞,极少分化为TH阳性神经元,与rBMSCs相比对PD大鼠模型有明显治疗作用。

Interfacial Modification of NiO_(x)by Self-assembled Monolayer for Efficient and Stable Inverted Perovskite Solar Cells

NiO_(x)as a hole transport material for inverted perovskite solar cells has received great attention owing to its high transparency,low fabrication temperature,and superior stability.However,the mismatched energy levels and possible redox reactions at the NiO_(x)/perovskite interface severely limit the performance of NiO_(x) based inverted perovskite solar cells.Herein,we introduce a p-type self-assembled monolayer between NiO_(x)and perovskite layers to modify the interface and block the undesirable redox reaction between perovskite and NiO_(x)The selfassembled monolayer molecules all contain phosphoric acid function groups,which can be anchored onto the NiOr surface and passivate the surface defect.Moreover,the introduction of self-assembled monolayers can regulate the energy level structure of NiO_(x),reduce the interfacial band energy offset,and hence promote the hole transport from perovskite to NiO_(x)layer.Consequently,the device performance is significantly enhanced in terms of both power conversion efficiency and stability.

A new approach for recycling arsenic and tin from low-grade tin middlings using a self-sulfurization roasting

Massive amounts of low-grade tin middlings have been produced from tin tailings,in which arsenic and tin are worthy to be recycled.Owing to high sulfur content in these tin middlings,a novel self-sulfurization roasting was proposed to transform,separate and recover arsenic and tin in this research.There was no extra curing agent to be added,which decreased the formation of pollutant S-containing gas.The self-sulfurization process involved a two-stage roasting of reduction followed by sulfurization.First in reduction roasting,FeAsS decomposed to FeS and As and the As then transformed to As_(4)(g)and As_(4)S_(4)(g),via which the arsenic was separated and recovered.The arsenic content in the first residue could be decreased to 0.72 wt.%.Accompanied with it,the FeS was firstly oxidized to Fe_(1−x)S and then to SO_(2)(g)by the coexisted Fe_(2)O_(3),and finally reduced and combined with the independent Fe_(2)O_(3)to form Fe_(1−x)S.In the followed sulfurization roasting,the Fe_(1−x)S sulfurized SnO_(2)to SnS(g),due to which tin could be recovered and its content in the second residue decreased to 0.01 wt.%.This study provided an efficient method to separate and recover arsenic and tin from low-grade tin middlings.

大鼠骨髓间充质干细胞的培养及初步鉴定

目的探讨体外获取高纯度大鼠骨髓间充质干细胞(BMSCs)方法以及通过形态学观察BMSCs在培养过程中是否存在自分化现象。方法应用全骨髓贴壁法分离培养大鼠BMSCs,倒置显微镜下观察细胞形态,直接荧光抗体染色法鉴定培养的细胞,免疫细胞化学方法鉴定其向神经元的诱导分化。结果原代末期及传代的BMSCs呈均一长梭形生长,第三代荧光抗体染色CD29,CD90阳性表达,神经元特异性标记物Nse(神经元特异性烯醇化酶)表达阳性,BMSCs在未加任何诱导剂时培养30d时发生了自分化现象。结论贴壁培养法是一种有效的获得高纯度BMSCs的方法,在体外培养时BMSCs可发生自分化现象。

Fabricating Core-Shell WC@C/Pt Structures and its Enhanced Performance for Methanol Electrooxidation

The spray-dried spheres within a W/Pt multi-separation can be used to prepare discrete core-shell WC@C/Pt catalysts through a typical carburization production mechanism at 800 ℃. In contrast with previous studies of the WC/Pt synthesis, the reaction observed here proceeds through an indirect annealing thereby resulting in core-shell structure, and mechanism at 600℃ wherein species diffuse, Pt nanoparticles were successfully dispersed in size/shape and randomly scattered across the in situ produced C spheres. Through direct carburization or at higher initial hydrochloroplatiuic acid concentrations, however, complete reaction with core-shell spheres was not observed. Indirect carburization reduces the strain felt by the bonds featuring the larger WC WC and Pt nanoparticles to be reserved, stability toward methanol oxidation. particles and allows the motion of carbon around influencing the eleetrocatalytic performance and

Use of Solid SMEDDS in Delivery of Carvedilol

Aim A new solid SMEDDS (self-microemulsifying drug delivery system) capsule has been developed to increase the solubility and dissolution rate. Methods The solubilities of carvedilol in various bases were investigated. Ternary phase diagrams were used to evaluate the self-emulsification and self-microemulsfication domains. The particle size distribution and ζ-potential were determined. The mean diameter of the three formulae decreased with an increase of Lutrol F68. Results The in vitro dissolution rate of ...

Robust adaptive polarization analysis method for eliminating ground roll in 3C land seismics

To improve the data quality of converted waves, and better identify and suppress the strong ground-roll interference in three-component （3C） seismic recordings on land, we present an adaptive polarization filtering method, which can effectively separate the ground- roll interference by combining complex polarization and instantaneous polarization analysis. The ground roll noise is characterized by elliptical plane polarization, strong energy, low apparent velocity, and low frequency. After low-pass filtering of the 3C data input within a given time-window of the ground roll, the complex covariance matrix is decomposed using the sliding time window with overlapping data and length that depends on the dominant ground-roll frequency. The ground-roll model is established using the main eigenvectors, and the ground roll is detected and identified using the instantaneous polarization area attributes and average energy constraints of the ground-roll zone. Finally, the ground roll is subtracted. The threshold of the method is stable and easy to select, and offers good ground- roll detection. The method is a robust polarization filtering method. Model calculations and actual data indicate that the method can effectively identify and attenuate ground roll while preserving the effective signals.

Apparent activation energy for spontaneous combustion of sulfide concentrates in storage yard

In order to evaluate the spontaneous combustion hazard of sulfide concentrates in storage, three different kinds of sulfide concentrates （sulfur-rich sulfide concentrate, iron sulfide concentrate and copper sulfide concentrate） were obtained from a storage yard in Dongguashan Copper Mine, China. The reaction processes at different heating rates of 5, 10, 15, 20, and 25 ℃/min in air flow from ambient temperature to 1 000 ℃ were studied by TG-DTG-DSC analysis. By the peak temperatures of DTG curves, the whole reaction process for each sample was divided into different stages, and the corresponding apparent activation energies were calculated by the Ozawa-Flynn-Wall method. It is found that the reaction process of each sample is considerably complex; the apparent activation energy values change from 36 to 160 kJ/mol in different temperature ranges; sulfur-rich sulfide and iron sulfide concentrates have lower apparent activation energy than copper sulfide concentrate below 150 ℃; so they are more inclined to cause spontaneous combustion at ambient temperature.

Knowledge-Based Classification in Automated Soil Mapping

A machine-learning approach was developed for automated building of knowledgebases for soil resources mapping by using a classification tree to generate knowledge from trainingdata. With this method, building a knowledge base for automated soil mapping was easier than usingthe conventional knowledge acquisition approach. The knowledge base built by classification tree wasused by the knowledge classifier to perform the soil type classification of Longyou County,Zhejiang Province, China using Landsat TM bi-temporal images and CIS data. To evaluate theperformance of the resultant knowledge bases, the classification results were compared to existingsoil map based on a field survey. The accuracy assessment and analysis of the resultant soil mapssuggested that the knowledge bases built by the machine-learning method was of good quality formapping distribution model of soil classes over the study area.

An improved TF-IDF approach for text classification

This paper presents a new improved term frequency/inverse document frequency (TF-IDF) approach which uses confidence, support and characteristic words to enhance the recall and precision of text classification. Synonyms defined by a lexicon are processed in the improved TF-IDF approach. We detailedly discuss and analyze the relationship among confidence, recall and precision. The experiments based on science and technology gave promising results that the new TF-IDF approach improves the precision and recall of text classification compared with the conventional TF-IDF approach.

Proton-gradient-transfer acid complexes and their catalytic performance for the synthesis of geranyl acetate

Special proton-gradient-transfer acid complexes （PGTACs） in which the bonded protons are not equivalent and have gradients in transfer ability, acidity, and reactivity were reported. The acidity gradient of the protons gave the PGTACs excellent catalytic activity and selectivity in the esterifica- tion of terpenols. These PGTACs are ＂reaction-induced self-separation catalysts＂ and can be easily reused. The kinetics with PGTACs as catalyst in the esterification of geraniol were also studied for use in engineering design.

Maximum Hydrogen Production by Autothermal Steam Reforming of Bio-oil With NiCuZnAI Catalyst

Autothermal steam reforming （ATR） of bio-oil, which couples the endothermic steam reform- ing reaction with the exothermic partial oxidation, offers many advantages from a technical and economic point of view. Effective production of hydrogen through ATR of bio-oil was performed at lower temperature with NiCuZnAl catalyst. The highest hydrogen yield from bio-oil reached 64.3% with a nearly complete bio-oil conversion at 600℃, the ratio of steam to carbon fed （S/C） of 3 and the oxygen to carbon ratio （O/C） of 0.34. The reaction conditions in ATR including temperature, O/C, S/C and weight hourly space velocity can be used to control both hydrogen yield and products distribution. The comparison between the ATR and common steam reforming of bio-oil was studied. The mechanism of the ATR of bio-oil was also discussed.

Urban Expansion in China Based on Remote Sensing Technology：A Review

Urban areas and its evolution are important anthropogenic indicators and human ecological footprints, and play decisive roles in environmental change analysis, global geo-conditional monitoring, and sustainable development. China has the highest rate of urban expansion and has emerged as an urban expansion hotspot worldwide. In this paper, the progress of studies on Chinese urban expansion based on remote sensing technology are summarized and analyzed from the aspects of urban area definition, remotely sensed imagery applied in urban expansion, monitoring methods of urban expansion, and urban expansion applications. Existing issues and future directions of Chinese urban expansion are discussed and proposed. Results indicate that: 1) The fusion of multi-source remotely sensed imagery is imperative to meet the needs of urban expansion with various monitoring terms and frequencies on different scales and dimensions. 2) To guarantee the classification accuracy and efficiency and describe urban expansion and its influences on local land use simultaneously, the combination of visual interpretation and automatic classification is the tendency of future monitoring methods of urban areas. 3) Urban expansion data have become the prerequisite for recognizing the urban development process, excavating its driving forces, simulating and predicting the future development directions, and also is conducive to revealing and explaining urban ecological and environmental issues. 4) In the past decades, Chinese scholars have promoted the application of remote sensing technology in the urban expansion field, with data construction, methods and models developing from the quotation stage to improvement and innovation stage; however, an independent and consistent urban expansion data on the national scale with long-term and high-frequency(such as annual monitoring) monitoring is still lacking.

Decentralized H_∞ state feedback control for large-scale interconnected uncertain systems with multiple delays

Decentralized H_∞ control was studied for a class of interconnected uncertain systems with multiple delays in the state and control and time varying but norm-bounded parametric uncertainties. A sufficient condition which makes the closed-loop system decentralized asymptotically stable with H_∞ performance was derived based on Lyapunov stability theorem. This condition is expressed as the solvability problem of linear matrix inequalities. The method overcomes the limitations of the existing algebraic Riccati equation method. Finally, a numerical example was given to demonstrate the design procedure for the decentralized H_∞ state feedback controller.

Two clinically relevant pressures of carbon dioxide pneumoperitoneum cause hepatic injury in a rabbit model

AIM:To observe the hepatic injury induced by carbon dioxide pneumoperitoneum(CDP) in rabbits,compare the eects olow-and high-pressure pneumoperitoneum,and to determine the degree o hepatic injury induced by these two clinically relevant CDP pressures.METHODS:Thirty healthy male New Zealand rabbits weighing 3.0 to 3.5 kg were randomly divided into three groups(n = 10 for each group) and subjected to the ollowing to CDP pressures:no gas control,10 mmHg,or 15 mmHg.Histological changes in liver tissues were observed with hematoxylin and eosin staining and transmission electron microscopy.Liver unction was evaluated using an automatic biochemical analyzer.Adenine nucleotide translocator(ANT) activity in liver tissue was detected with the atractyloside-inhibitor stop technique.Bax and Bcl-2 expression levels were detected bywestern blotting.RESULTS:Liver Functions in the 10 mmHg and 15 mmHg experimental groups were significantly disturbed compared with the control group.After CDP,the levels or alanine transaminase and aspartate transaminase were 77.3 ± 14.5 IU/L and 60.1 ± 11.4 IU/L,respectively,in the 10 mmHg experimental group and 165.1 ± 19.4 IU/L and 103.8 ± 12.3 IU/L,respectively,in the 15 mmHg experimental group,which were all higher than those of the control group(p < 0.05).There was no difference in pre-albumin concentration between the 10 mmHg experimental group and the control group,but the prealbumin level of the 15 mmHg experimental group was significantly lower than that of the control group(p < 0.05).No significant differences were observed in the levels of total bilirubin or albumin among the three groups.After 30 and 60 min of CDP,pH was reduced(p < 0.05) and fa CO2 was elevated(p < 0.05) in the 10 mmHg group compared with controls,and these changes were more pronounced in the 15 mmHg group.Hematoxylin and eosin staining showed no significant change in liver morphology,except for mild hyperemia in the two experimental groups.Transmission electron microscopy showed mild mitochondrial swelling in hepatocytes of the 10 mmHg group,and this was more pronounced in the 15 mmHg group.No significant difference in ANT levels was found between the control and 10 mmHg groups.However,ANT concentration was significantly lower in the 15 mmHg group compared with the control group.The expression of hepatic Bax was significantly increased in the two experimental groups compared with the controls,but there were no differences in Bcl-2 levels among the three groups.Twelve hours after CDP induction,the expression of hepatic Bax was more significant in the 15 mmHg group than in the 10 mmHg group.CONCLUSION:A CDP pressure of 15 mmHg caused more substantial hepatic injury,such as increased levels of acidosis,mitochondrial damage,and apoptosis;therefore,10 mmHg CDP is preferable for laparoscopic operations.

Calculation of Environmental Capacity of Petroleum Hydrocarbon in Jiaozhou Bay

The method has been established to calculate the environmental capacity (ECO), surplus environment capacity (SECO) of water with respect to marine petroleum hydrocarbons associated with oil (PHAOs) and the self-purification capacity (SPCO) of main self-purification process to PHAOs in the Jiaozhou Bay, China, according to the dynamic model for distribution of marine PHAOs among multiphase environments. The variation of concentration of PHAOs in the Jiaozhou Bay is well simulated by the dynamic model. Based on the model, the ECo, SECo of water with respect to PHAOs in the Jiaozhou Bay were calculated during the last 10 years under the first-class and second-class quality standard requirement, according to SPCO of main self-purification process to PHAOs. The results show that about 200 tons of PHAOs could be discharged into the Jiaozhou Bay for maintaining the first class seawater quality standard, and about 600 tons of PHAOs for the second class seawater quality standard later.

Observer-based Iterative and Repetitive Learning Control for a Class of Nonlinear Systems

In this paper, both output-feedback iterative learning control(ILC) and repetitive learning control(RLC) schemes are proposed for trajectory tracking of nonlinear systems with state-dependent time-varying uncertainties. An iterative learning controller, together with a state observer and a fully-saturated learning mechanism, through Lyapunov-like synthesis, is designed to deal with time-varying parametric uncertainties. The estimations for outputs, instead of system outputs themselves, are applied to form the error equation, which helps to establish convergence of the system outputs to the desired ones. This method is then extended to repetitive learning controller design. The boundedness of all the signals in the closed-loop is guaranteed and asymptotic convergence of both the state estimation error and the tracking error is established in both cases of ILC and RLC. Numerical results are presented to verify the effectiveness of the proposed methods.

Hydraulic cylinder control of injection molding machine based on differential evolution fractional order PID

Injection molding machine,hydraulic elevator,speed actuators belong to variable speed pump control cylinder system.Because variable speed pump control cylinder system is a nonlinear hydraulic system,it has some problems such as response lag and poor steady-state accuracy.To solve these problems,for the hydraulic cylinder of injection molding machine driven by the servo motor,a fractional order proportion-integration-diferentiation(FOPID)control strategy is proposed to realize the speed tracking control.Combined with the adaptive differential evolution algorithm,FOPID control strategy is used to determine the parameters of controller on line based on the test on the servo-motor-driven gear-pump-controlled hydraulic cylinder injection molding machine.Then the slef-adaptive differential evolution fractional order PID controller(SADE-FOPID)model of variable speed pump-controlled hydraulic cylinder is established in the test system with simulated loading.The simulation results show that compared with the classical PID control,the FOPID has better steady-state accuracy and fast response when the control parameters are optimized by the adaptive differential evolution algorithm.Experimental results show that SADE-FOPID control strategy is effective and feasible,and has good anti-load disturbance performance.

Catalyst-free and solvent-free oxidation of cycloalkanes(C5-C8) with molecular oxygen:Determination of autoxidation temperature and product distribution

Autoxidation of cycloalkanes （C5-C8） with molecular oxygen under catalyst-free and solvent-free conditions was conducted systematically for the first time, focusing on the autoxidation temperature and product distribution. The autoxidation of cyclopentane, cyclohexane, cycloheptane and cyclooctane occurs at 120 ℃, 130 ℃, 120 ℃, and 105 ℃ respectively, with obvious oxidized products formation. At 140 ℃, 145 ℃, 130 ℃ and 125 ℃, acceptable yields of the oxidized products could be obtained for them, and the oxidized product distributions were investigated in detail. The autoxidation of cycloalkanes follows the pseudo-first-order kinetic model and the apparent activation energies （Ea） for the autoxidation of cyclopentane and cyclohexane are 159.76 kJ. tool-1 and 86.75 kJ. mol-1 respectively. This study can act as an important reference in screen of suitable reaction temperature and comparison of the performance of various catalysts in the catalytic oxidation of cycloalkanes in the attempt to enhance the oxidized product selectivity.

Coarse-Grained Free-Energy Simulations of Conformational State Transitions in an Adenosine 5′-Triphosphate-binding Cassette Exporter

ATP-binding cassette exporters transport many substrates out of cellular membranes via alternating between inward-facing and outward-facing conformations. Despite extensive research efforts over the past decades, understanding of the molecular mechanism remains elusive. As these large-scale conformational movements are global and collective, we have previously performed extensive coarse-grained molecular dynamics simulations of the potential of mean force along the conformational transition pathway [J. Phys. Chem. B 119, 1295(2015)]. However, the occluded conformational state, in which both the internal and external gate are closed, was not determined in the calculated free energy profile. In this work, we extend the above methods to the calculation of the free energy profile along the reaction coordinate, d1-d2, which are the COM distances between the two sides of the internal(d1)and the external gate(d2). The potential of mean force is thus obtained to identify the transition pathway, along which several outward-facing, inward-facing, and occluded state structures are predicted in good agreement with structural experiments. Our coarse-grained molecular dynamics free-energy simulations demonstrate that the internal gate is closed before the external gate is open during the inward-facing to outward-facing transition and vice versa during the inward-facing to outward-facing transition. Our results capture the unidirectional feature of substrate translocation via the exporter, which is functionally important in biology. This finding is different from the previous result, in which both the internal and external gates are open reported in an X-ray experiment [Proc. Natl. Acad. Sci. USA 104,19005(2007)]. Our study sheds light on the molecular mechanism of the state transitions in an ATP-binding cassette exporter.