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磁珠DNA自动提取系统在法医检验中的应用 被引量:3
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作者 管桦 李英 +2 位作者 荣海博 赵颖 阮德林 《刑事技术》 2013年第5期29-32,共4页
磁珠DNA自动提取系统是将磁珠分离技术与DNA自动提取工作站结合运用,以达到高效提取DNA的目的,它具有操作时间短、准确性高、检材用量少等特点,特别适用于大批量生物检材的快速提取,是目前主流的DNA自动化提取方法。本文对磁珠DNA自动... 磁珠DNA自动提取系统是将磁珠分离技术与DNA自动提取工作站结合运用,以达到高效提取DNA的目的,它具有操作时间短、准确性高、检材用量少等特点,特别适用于大批量生物检材的快速提取,是目前主流的DNA自动化提取方法。本文对磁珠DNA自动提取系统的基本组成、工作原理、操作流程及其在法医检验领域中的应用等进行了综述,并对该系统的发展前景进行了展望。 展开更多
关键词 法医物证学 DNA自动提取系统 磁珠 法医检验
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人脸图像嘴巴特征点自动提取系统 被引量:3
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作者 胡迎春 张增芳 梁数 《广西工学院学报》 CAS 2003年第2期27-30,共4页
利用彩色人脸的特点 ,设计一个初步的人脸图像嘴巴特征点的自动提取系统。通过水平投影和垂直投影确定嘴巴的矩形区域 ,然后在嘴巴所在的矩形区域内提取 6个特征点 ,并且由这 6个点拟合五段二次抛物线 ,由此描绘出嘴巴的边缘轮廓。实验... 利用彩色人脸的特点 ,设计一个初步的人脸图像嘴巴特征点的自动提取系统。通过水平投影和垂直投影确定嘴巴的矩形区域 ,然后在嘴巴所在的矩形区域内提取 6个特征点 ,并且由这 6个点拟合五段二次抛物线 ,由此描绘出嘴巴的边缘轮廓。实验证明 。 展开更多
关键词 人脸识别 嘴巴图像 图象处理 特征点 自动提取系统 投影 抛物线拟和 边缘轮廓 中值滤波 彩色图像
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国产磁珠核酸自动提取系统对HBV-DNA检测前处理的研究 被引量:9
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作者 易勇 赵焕生 +4 位作者 贾莉萍 魏平 尹玉峰 聂棱 敬华 《中国卫生检验杂志》 CAS 2011年第8期1906-1908,共3页
目的:比较国产磁珠法核酸自动提取系统和传统的沉淀离心提取法提取血清HBV DNA的效果,探讨其应用潜力。方法:以两种方法平行提取50份血清HBV DNA,荧光定量PCR检测其提取核酸的拷贝数及CT值,进行方法对比试验;配制成不同浓度的HBV应用血... 目的:比较国产磁珠法核酸自动提取系统和传统的沉淀离心提取法提取血清HBV DNA的效果,探讨其应用潜力。方法:以两种方法平行提取50份血清HBV DNA,荧光定量PCR检测其提取核酸的拷贝数及CT值,进行方法对比试验;配制成不同浓度的HBV应用血清,用国产自动提取系统提取HBV DNA,评价方法的灵敏度、重复性及线性范围;向已知浓度的HBV阳性血清中定量加入溶血及脂血的阴性血清,再同样提取HBV DNA,观察方法的抗干扰特性。结果:两种方法平行提取血清HBV DNA,方法对比试验显示无显著差别;国产自动提取系统的重复性良好,定量检测的灵敏度及线性范围与现行方法一致;抗干扰能力强,溶血及脂血等常见干扰物对HBV DNA提取无干扰。结论:国产磁珠法核酸自动提取系统对血清HBV DNA的提取效果良好,操作简单,自动化程度高,重复性好,适宜用于临床HBV DNA检测前处理的核酸提取。 展开更多
关键词 国产设备 磁珠 核酸自动提取系统 HBVDNA
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一种国产磁珠核酸自动提取系统对人巨细胞病毒DNA检测的性能评价 被引量:2
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作者 毕昊 吴岚 +5 位作者 郭秋 张然迪 夏剑波 吴江 柯斌 尹合奎 《临床血液学杂志(输血与检验)》 CAS 2020年第4期529-533,共5页
目的:评价一种国产核酸磁珠自动提取系统对人巨细胞病毒(HCMV)DNA检测的性能。方法:采用科华磁珠核酸自动提取仪提取血浆HCMV DNA,经实时荧光定量PCR检测,分析其线性范围、灵敏度、精密度及抗干扰能力、8混养模式的检测效能。并利用30份... 目的:评价一种国产核酸磁珠自动提取系统对人巨细胞病毒(HCMV)DNA检测的性能。方法:采用科华磁珠核酸自动提取仪提取血浆HCMV DNA,经实时荧光定量PCR检测,分析其线性范围、灵敏度、精密度及抗干扰能力、8混养模式的检测效能。并利用30份含HCMV感染患者及20份健康人群血浆对该方法进行临床应用评价,其检测结果与沉淀离心提取法进行比较。结果:该方法检测HCMV DNA载量的线性范围为102~106 copy/mL。检测灵敏度为100 copy/mL(单检)。测定值与理论值的相关性良好,R2=0.998,P<0.01。批内和批间变异系数(CV)均小于5%。临床应用评价发现,国产磁珠核酸自动提取法与沉淀离心提取法对临床样品的检测一致性较好(Kappa=0.839≥0.75,P<0.01),磁珠自动提取法的阳性率高于沉淀离心提取法(100%vs 86.7%),但差异无统计学意义(P=0.125)。在102~103 copy/mL范围内,国产磁珠核酸自动提取发现优于沉淀离心法。2法所获得的定量结果差异有统计学意义(P<0.05)。8混养模式的检测灵敏度为103 copy/mL,拆分结果与实际相符,未产生交叉污染。结论:基于国产磁珠自动核酸提取系统实现了快速、自动化提取HCMV DNA,具有良好检测性能,适用于采供血机构HCMV的血液核酸检测及临床应用推广。 展开更多
关键词 人巨细胞病毒 磁珠 核酸自动提取系统 性能评价
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磁珠DNA自动提取系统在法医检验中的应用研究
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作者 岳希臻 《中文科技期刊数据库(全文版)医药卫生》 2021年第7期9-10,共2页
磁珠DNA自动提取法不仅操作方法较简单,无需特殊设备,避免了大样品反复离心的损失,且实用范围较广,可应用于多种生物样本的提取,如唾液、汗斑、血斑、精斑等。目前已大量应用在法医检验中,对案件侦破效用极大。本文对磁珠DNA自动提取系... 磁珠DNA自动提取法不仅操作方法较简单,无需特殊设备,避免了大样品反复离心的损失,且实用范围较广,可应用于多种生物样本的提取,如唾液、汗斑、血斑、精斑等。目前已大量应用在法医检验中,对案件侦破效用极大。本文对磁珠DNA自动提取系统进行了概述,分析了其重要性及优势,简述了在法医检验中的应用及展望,报道如下。 展开更多
关键词 磁珠DNA 自动提取系统 法医检验
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磁珠法提取丙肝RNA的交叉污染风险评价
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作者 刘歆 《临床医学研究与实践》 2020年第16期106-107,共2页
目的考察EX3600核酸自动提取仪提取丙型肝炎病毒核酸(HCV-RNA)时防交叉污染的能力。方法使用EX3600核酸自动提取仪以阴性、阳性相交的方式上样提取HCV-RNA,通过对提取样本进行PCR定量检测确定阴性样本在提取过程中是否被阳性样本污染。... 目的考察EX3600核酸自动提取仪提取丙型肝炎病毒核酸(HCV-RNA)时防交叉污染的能力。方法使用EX3600核酸自动提取仪以阴性、阳性相交的方式上样提取HCV-RNA,通过对提取样本进行PCR定量检测确定阴性样本在提取过程中是否被阳性样本污染。结果阳性样本均检出,阴性样本均未检出。结论本研究在一定程度上证明了EX3600自动核酸提取仪的防污染能力,为保证结果的准确性提供依据。 展开更多
关键词 自动核酸提取系统 HCV-RNA 交叉污染
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两种提取系统对牙齿DNA检验效果比较 被引量:1
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作者 刘光仁 《中国法医学杂志》 CSCD 2018年第5期516-517,515,共3页
目的通过本次实验对比两种常用的DNA自动化提取系统对牙齿DNA纯化的效果。方法采用博坤微量生物物证DNA自动化提取系统(整合版)和Automate Express BTA自动化法医DNA提取系统分别对同一人的牙齿进行检验。结果经博坤微量生物物证DNA自... 目的通过本次实验对比两种常用的DNA自动化提取系统对牙齿DNA纯化的效果。方法采用博坤微量生物物证DNA自动化提取系统(整合版)和Automate Express BTA自动化法医DNA提取系统分别对同一人的牙齿进行检验。结果经博坤微量生物物证DNA自动化提取系统(整合版)检验的牙齿DNA图谱峰高平均值约为Automate Express BTA自动化法医DNA提取系统的2倍;等位基因个数平均值约为1.5倍。结论博坤微量生物物证DNA自动化提取系统(整合版)代替传统手工提取方法检验牙齿,相较于Automate Express BTA自动化法医DNA提取系统提取效果更好,能满足实际检案的要求,可在法医学实践中选择使用。 展开更多
关键词 法医物证学 STR分型 自动化法医DNA提取系统 牙齿
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Fingerprint singular points extraction based on orientation tensor field and Laurent series 被引量:3
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作者 刘琴 彭可 +4 位作者 刘巍 谢琴 李仲阳 兰浩 金耀 《Journal of Central South University》 SCIE EI CAS 2014年第5期1927-1934,共8页
Singular point(SP)extraction is a key component in automatic fingerprint identification system(AFIS).A new method was proposed for fingerprint singular points extraction,based on orientation tensor field and Laurent s... Singular point(SP)extraction is a key component in automatic fingerprint identification system(AFIS).A new method was proposed for fingerprint singular points extraction,based on orientation tensor field and Laurent series.First,fingerprint orientation flow field was obtained,using the gradient of fingerprint image.With these gradients,fingerprint orientation tensor field was calculated.Then,candidate SPs were detected by the cross-correlation energy in multi-scale Gaussian space.The energy was calculated between fingerprint orientation tensor field and Laurent polynomial model.As a global descriptor,the Laurent polynomial coefficients were allowed for rotational invariance.Furthermore,a support vector machine(SVM)classifier was trained to remove spurious SPs,using cross-correlation coefficient as a feature vector.Finally,experiments were performed on Singular Point Detection Competition 2010(SPD2010)database.Compared to the winner algorithm of SPD2010 which has best accuracy of 31.90%,the accuracy of proposed algorithm is 45.34%.The results show that the proposed method outperforms the state-of-the-art detection algorithms by large margin,and the detection is invariant to rotational transformations. 展开更多
关键词 fingerprint extraction singular point fingerprint orientation tensor field Laurent series rotational invariance supportvector machine (SVM)
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Field-Based Automated Survey for Extracting Tree Inventory Attributes and 3-D Models in a Forested Area
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作者 Jasmee Jaafar Juazer Rizal Abdul Hamid Roslina ldris Azman All M. Habib 《Journal of Earth Science and Engineering》 2012年第9期561-570,共10页
Acquisition of tree inventory parameters such as tree position with respect to a local reference coordinate system, tree height, dbh (diameter breast height) and other associated tree attributes is a tedious process... Acquisition of tree inventory parameters such as tree position with respect to a local reference coordinate system, tree height, dbh (diameter breast height) and other associated tree attributes is a tedious process, time consuming, costly and labour intensive. An automatic collection and processing in the field could expedite the process of tree inventory survey and data management. The advent of handheld laser equipments such as the MDL LaserAce 300, should allow rapid acquisition of tree attributes. The instrument measures distances, differences in height and horizontal bearing automatically. This paper describes a study about the automated process of retrieving tree positions, their respective attributes and the creation of 3-D model (three-dimensional model). A software developed in-house known as ASSIST (automated spatial survey information system) was utilized together with the MI)I, LaserAce 300, being the hardware component of the automated system. The capability of MDL LaserAce 9300 in extracting information regarding tree inventory attributes and the 3-D model encompassing the study area was investigated. The results were checked against the data sets acquired by a total station traversing and tacheometric survey respectively. The accuracy of the horizontal and vertical position of points gathered was critically assessed. The accuracy of the MDL LaserAce 300 was found to be less than ±2 m for both planimetric (horizontal) and vertical (height) in the construction of the 3-D model. The study demonstrates the effectiveness of a field-based automation system as a viable option to support forest application requirement. 展开更多
关键词 Measuring laser tree inventory 3-D model.
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Network Traffic Signature Generation Mechanism Using Principal Component Analysis
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作者 牟澄 黄小红 +1 位作者 吴军 马严 《China Communications》 SCIE CSCD 2013年第11期95-106,共12页
The Deep Packet Inspection(DPI)method is a popular method that can accurately identify the flow data and its corresponding application.Currently,the DPI method is widely used in common network management systems.Howev... The Deep Packet Inspection(DPI)method is a popular method that can accurately identify the flow data and its corresponding application.Currently,the DPI method is widely used in common network management systems.However,the major limitation of DPI systems is that their signature library is mainly extracted manually,which makes it hard to efficiently obtain the signature of new applications.Hence,in this paper,we propose an automatic signature extraction mechanism using Principal Component Analysis(PCA)technology,which is able to extract the signature automatically.In the proposed method,the signatures are expressed in the form of serial consistent sequences constructed by principal components instead of normally separated substrings in the original data extracted from the traditional methods.Extensive experiments based on numerous sets of data have been carried out to evaluate the performance of the proposed scheme,and the results prove that the newly proposed method can achieve good performance in terms of accuracy and efficiency. 展开更多
关键词 network traffic classification aut-omatic signature generation deep packet in-spection principal component analysis
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Current issues in high-resolution earth observation technology 被引量:20
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作者 LI DeRen TONG QingXi +2 位作者 LI RongXing GONG JianYa ZHANG LiangPei 《Science China Earth Sciences》 SCIE EI CAS 2012年第7期1043-1051,共9页
This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth obser... This paper reviewed the developments of the last ten years in the field of international high-resolution earth observation, and introduced the developmental status and plans for China's high-resolution earth observation program. In addition, this paper expounded the transformation mechanism and procedure from earth observation data to geospatial information and geographical knowledge, and examined the key scientific and technological issues, including earth observation networks, high-precision image positioning, image understanding, automatic spatial information extraction, and focus services. These analyses provide a new impetus for pushing the application of China's high-resolution earth observation system from a "quantity" to "quality" change, from China to the world, from providing products to providing online service. 展开更多
关键词 high-resolution earth observation sensor networks precision processing of remote sensing images automatic interpretation of remote sensing images focus services for spatial information
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