Autophagy is a vacuolar process of cytoplasmic degradation by lysosome which ubiquitously occurring in all eukaryotic cells. The researches of autophagy have made great progress with the development of the yeast model...Autophagy is a vacuolar process of cytoplasmic degradation by lysosome which ubiquitously occurring in all eukaryotic cells. The researches of autophagy have made great progress with the development of the yeast model and genetic technology. This review will summarize the determination of autophagy,its relationship with apoptosis and its role in the tumor treatment in order to give a comprehensive understanding of the function of autophagy.展开更多
目的:探讨正常子宫内膜、子宫内膜增生以及子宫内膜腺癌中自噬活性的变化。方法:收集2004年1月至2005年3月行子宫切除或诊断性刮宫的组织标本135例,其中正常子宫内膜43例,子宫内膜单纯性增生及复合性增生27例,子宫内膜不典型增生26例,...目的:探讨正常子宫内膜、子宫内膜增生以及子宫内膜腺癌中自噬活性的变化。方法:收集2004年1月至2005年3月行子宫切除或诊断性刮宫的组织标本135例,其中正常子宫内膜43例,子宫内膜单纯性增生及复合性增生27例,子宫内膜不典型增生26例,子宫内膜腺癌39例。用免疫组化法检测全部组织中的Cathepsin-D和Rab7的表达。结果:(1)Cathepsin-D表达在正常子宫内膜增生期明显高于分泌期(0.244±0.091 vs 0.141±0.071,P<0.05);Rab7表达呈同一趋势(0.197±0.084 vs 0.114±0.060,P<0.05);(2)子宫内膜单纯性、复合性增生组的Cathepsin-D表达明显高于子宫内膜不典型增生组,子宫内膜腺癌细胞中Cathepsin-D表达则明显减弱(0.561±0.168 vs0.311±0.089 vs 0.055±0.044,P<0.001);Rab7的表达呈现与Cathepsin-D同一趋势(0.508±0.152 vs 0.281±0.076 vs 0.026±0.019,P<0.001)。结论:正常增生期子宫内膜细胞的自噬活性比分泌期子宫内膜细胞明显增强,子宫内膜增生的自噬活性比正常子宫内膜强,而子宫内膜腺癌细胞自噬活性明显减弱,提示自噬活性的改变可能参与了子宫内膜样腺癌的发生。展开更多
文摘Autophagy is a vacuolar process of cytoplasmic degradation by lysosome which ubiquitously occurring in all eukaryotic cells. The researches of autophagy have made great progress with the development of the yeast model and genetic technology. This review will summarize the determination of autophagy,its relationship with apoptosis and its role in the tumor treatment in order to give a comprehensive understanding of the function of autophagy.
文摘目的:探讨正常子宫内膜、子宫内膜增生以及子宫内膜腺癌中自噬活性的变化。方法:收集2004年1月至2005年3月行子宫切除或诊断性刮宫的组织标本135例,其中正常子宫内膜43例,子宫内膜单纯性增生及复合性增生27例,子宫内膜不典型增生26例,子宫内膜腺癌39例。用免疫组化法检测全部组织中的Cathepsin-D和Rab7的表达。结果:(1)Cathepsin-D表达在正常子宫内膜增生期明显高于分泌期(0.244±0.091 vs 0.141±0.071,P<0.05);Rab7表达呈同一趋势(0.197±0.084 vs 0.114±0.060,P<0.05);(2)子宫内膜单纯性、复合性增生组的Cathepsin-D表达明显高于子宫内膜不典型增生组,子宫内膜腺癌细胞中Cathepsin-D表达则明显减弱(0.561±0.168 vs0.311±0.089 vs 0.055±0.044,P<0.001);Rab7的表达呈现与Cathepsin-D同一趋势(0.508±0.152 vs 0.281±0.076 vs 0.026±0.019,P<0.001)。结论:正常增生期子宫内膜细胞的自噬活性比分泌期子宫内膜细胞明显增强,子宫内膜增生的自噬活性比正常子宫内膜强,而子宫内膜腺癌细胞自噬活性明显减弱,提示自噬活性的改变可能参与了子宫内膜样腺癌的发生。
