Ca2+、Mg2+对剩余污泥自培养产酶影响

为提高剩余污泥投加复合菌后产水解酶效果,探讨了添加Ca^2+、Mg^2+对剩余污泥自培养产酶中污泥水解的影响。结果表明,未加Ca^2+、Mg^2+的空白组,蛋白酶和淀粉酶活力均呈现第1d上升至最大值,后持续降低的趋势。添加0.03mol/L Ca^2+组的蛋白酶活力第1d快速升至246.14U/mL,后持续上升,并在第7d酶活力达最大值340.07U/mL,蛋白酶活力最大值较空白组增加了53.94%;淀粉酶活力在第1d快速升至351.02U/mL后快速下降,第7d酶活力为70.79U/mL,淀粉酶活力最大值较空白组增加了51.93%,表明添加0.03mol/L Ca^2+可以提高灭菌污泥自培养产酶效果。而添加0.06mol/L Ca^2+组酶活力呈下降趋势,表明高浓度Ca^2+对产酶有抑制作用。添加0.03mol/L或0.06mol/L Mg^2+,不能提高产酶效果,但能维持酶活力稳定。添加合适浓度Ca^2+、Mg^2+能强化剩余污泥产酶过程中污泥水解效果,促进污泥上清液中有机物水解。

自培养乳酸菌动物饮料在畜牧业的应用

乳酸菌类微生物饲料添加剂被应用在畜牧业之中,获得了很好的成效。自培养乳酸菌动物饮料,其工艺步骤比较特殊,未来将成为一种在畜牧业中被广为应用的生产技术。乳酸菌为革兰氏阳性菌,其主要应用领域在于食品、饲料。本文将分析自培养乳酸菌动物饮料在畜牧业中的应用,从而能够为相关领域的发展提供理论依据和实践资料。

SBR策略控制活性污泥培养

着重分析了在以SBR方式启动的活性污泥自培养过程周期内的氨氮、亚硝酸盐、硝酸盐、SCOD、pH、DO的变化规律。结果表明,在供氧充足的条件下,出现亚硝酸盐的积累是正常的,也是短暂的;不影响培养过程中对污泥量增长的需要,在周期性进出水过程中,COD的去除率达到80%,氨氮的去除率达到100%。通过对这些指标的分析,对于缩短曝气时间,加速反应器的启动有极大的帮助。

How Can Self-Access Centers in China Better Develop Students＇ Learner Autonomy

Accompanying the arrival of new technological and network ear, SACs （self-access centers） become the most widely adopted mode spreading in China with a large quantity of universities joining to follow the fashion. But whether the SACs in China can fully fulfill the purpose, to what extend can learners gain benefits to develop autonomy, and what are the possible ways for improvement are quite necessary to investigate since there starts to emerge various problems during the implementation of SACs. This paper aims to analyze the prevalent establishment of SACs in China in order to find out the reasons for prevalence, the current implementing mode, the degree of effectiveness on developing learner autonomy, especially potential problems and causes, and finally put forward some suggestions pedagogical implications for improving.

The Chattering Charades of Nature and Nurture： State-of-the-Art in Nonverbal Communication and Its Relationship With Verbal Messages

Employing a qualitative view of issues in nonverbal communication, the author offers an explanation of the nature-nurture debate. The anthropological view, taken by Birdwhistell and others, is compared with a Darwinian perspective. In particular, the author looks at how physical appearance, space, and gestures function in a natural way to protect the individuals who are participating in the exchange. Apparent deceit is frequently the result of such interactions. While the traditional arguments arising from religious tenets are discussed, the author explains how the nature approach has been reinforced in the 20th and 21st centuries with DNA and neurological investigations. In the study of nonverbal communication, the arguments about nature and nurture began in the middle of the 20th century with anthropologists, especially Birdwhistell, taking the nurture stance and others taking the nature stance. Ekman＇s nature stance on kinesics started from premises suggested by Charles Darwin. In large measure, it is because of Ekman＇s extensive work that the ＂balance＂ has somewhat shifted toward a nature view.

Exploration and Construction of New ＂4-to-l＂ School- enterprise Cooperation Model in Private Colleges and Universities

The new ＂4-to-1＂ school-enterprise cooperation model is constructed in combination with the automation program provided by Tianshi College. The new school-enterprise cooperation model is an effective way for Tianshi College to train high-quality skilled personnel, an important carrier to deepen the production-study-research cooperation education, and also an important part to improve the work quality of personnel cultivation. In China, it is an urgent need of the current employment situation, but also an important strategy for Tianshi College to attain a lon^-term stable develot^ment. In the orocess of imolementation, a win-win situation has been achieved bv constantly deenening reform.

Research and building of optimization measures of college English teaching

more and more people recognize the speed and level of development of a university depends not only on the level of the school of thought and teacher but also on the laboratory construction and management level. Over the years many famous scholars and experts from many aspects and multidimensional perspective discusses the role and importance of laboratory work and practice of education and teaching, practice shows that： the degree of awareness and attention to a university laboratory construction and practice teaching in a sense reflects the understanding of the university personnel training and higher education. In the past, ＂focus on the theory and lighten the practice,＂ the idea has far-reaching impact, now we want to change old ideas; this is because in today＇ s quality education, construction, laboratory and practical education reform teaching content and methods are a part of education reform. We attach great importance to the English school laboratory building. In the new campus, the school has invested heavily to build five advanced equipment, it is a leading English Autonomous Learning Center. Independent Learning Centre is to stimulate students ＇interest in learning, develop students＇ ability to multi-faceted, full use modern teaching equipment to improve English skills platform of independent centers of learning effect.

Characteristics and Development Modes of Standardization forChina's National High-Tech Zones:A Review

Indigenous innovation focused on the development of technical standards has become the core of China's innovation policy,since China released the "Outline of the National Program for Longand Medium-Term Scientific and Technological Development (2006-2020)" in 2006.Meanwhile,standardization issues are greatly emphasized by high-tech zones which serve as the major carriers of national economic growth as well as innovation clusters in the context of fast-growing international standardization.By means of the effective guide of the government,the standardization of high-tech zones in China now presents a wide range of operating modes.Specifically,they can be divided predominantly into the government promotion mode,the alliance collaboration mode,the research-standard-industry linkage mode,and the talent training mode,in terms of the main body of work and influence factors.

Purification and identification of natural antikeratin autoantibody from normal human sera and its effects on the cultured keratinocytes

Objective: To purify the natural antikeratin autoantibody (AK auto-Ab) and observe its effects on the prolif eration of the cultured keratinocytes. Methods: Natural AK auto-Ab was purified by using keratin affinity column, and then the titre and specificity of the Abs were studied by ELISA, immunoperoxidase staining and immuno-electronicmicroscope. The effect of the purified Abs on the cultured keratino-cytes was assayed by 3H-TdR incorporation. Results: Natural AK auto-Ab was obtained. The binding activity of IgG AK auto-Ab in purified Ah remained similar to that in pooled human sera. and the specificity of the obtained antibody is strong. The purified antibody could decrease the Il-TdR incorporation of the cultured keratinocytes in a dose-dependent manner. Conclusion: The method of punning AK auto-Ab is simple, practicable and reliable. Natural AK auto-Ab, existing in normal human individuals, has inhibitory etiect on the proliferation of the cultured keratinocytes.

MONITORING OF METACOGNITIVE STRATEGY TRAINING

The goal of education is to cultivate learner autonomy. Studies in metacognition suggest that the ideal way to accomplish this is through metacognitive strategy training. Research also indicates that effective training programs depend on monitoring. To cultivate self-regulation or learner autonomy, it is necessary to exert external monitoring at the beginning, and gradually reduce external supervision as the students become more autonomous. The present study argues for cooperative group learning and regular evaluation as the main monitoring strategies. A semester long study indicates that cooperative group learning, besides being valuable in cultivating social relationship, and fostering cooperation and learner autonomy, can also serve as a good monitoring strategy in metacognitive strategy training. Regular evaluation, when it is progress- oriented and learner-centered, and conducted at proper times, will also provide valuable information for students to check, evaluate and adjust their learning. Self-questioning sheets and learner portfolios are also useful techniques in monitoring students' learning.

Comparative effects of plant growth regulators on leaf and stem explants of Labisia pumila var. alata

Objective: Labisia pumila var. alata, commonly known as 'Kacip Fatimah' or 'Selusuh Fatimah' in Southeast Asia, is traditionally used by members of the Malay community because of its post-partum medicinal properties. Its various pharmaceutical applications cause an excessive harvesting and lead to serious shortage in natural habitat. Thus, this in vitro propagation study investigated the effects of different plant growth regulators (PGRs) on in vitro leaf and stem explants of L. pumila. Methods: The capabilities of callus, shoot, and root formation were evaluated by culturing both explants on Murashige and Skoog (MS) medium supplemented with various PGRs at the concentrations of 0, 1, 3, 5, and 7 mg/L. Results: Medium supplemented with 3 mg/L indole-3-butyric acid (IBA) showed the optimal callogenesis from both leaf and stem explants with (72.34±19.55)% and (70.40±14.14)% efficacy, respectively. IBA was also found to be the most efficient PGR for root induction. A total of (50.00±7.07)% and (77.78±16.47)% of root formation were obtained from the in vitro stem and leaf explants after being cultured for (26.5±5.0) and (30.0±8.5) d in the medium supplemented with 1 and 3 mg/L of IBA, respectively. Shoot formation was only observed in stem explant, with the maximum percentage of formation ((100.00±0.00)%) that was obtained in 1 mg/L zeatin after (11.0±2.8) d of culture. Conclusions: Callus, roots, and shoots can be induced from in vitro leaf and stem explants of L. pumila through the manipulation of types and concentrations of PGRs.

Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response

The breakthrough development of induced pluripotent stem cells(iPSCs)raises the prospect of patient-specific treatment for many diseases through the replacement of affected cells.However,whether iPSC-derived functional cell lineages generate a deleterious immune response upon auto-transplantation remains unclear.In this study,we differentiated five human iPSC lines from skin fibroblasts and urine cells into neural progenitor cells(NPCs)and analyzed their immunogenicity.Through co-culture with autogenous peripheral blood mononuclear cells(PBMCs),we showed that both somatic cells and iPSC-derived NPCs do not stimulate significant autogenous PBMC proliferation.However,a significant immune reaction was detected when these cells were co-cultured with allogenous PBMCs.Furthermore,no significant expression of perforin or granzyme B was detected following stimulation of autogenous immune effector cells(CD3+CD8 T cells,CD3+CD8+T cells or CD3 CD56+NK cells)by NPCs in both PBMC and T cell co-culture systems.These results suggest that human iPSC-derived NPCs may not initiate an immune response in autogenous transplants,and thus set a base for further preclinical evaluation of human iPSCs.