In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts （MESFs）, clonally derived fibroblasts from the MESFs （CMESFs）, monkey oviductal fibroblasts （MOFs）, monkey follicular granulo...In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts （MESFs）, clonally derived fibroblasts from the MESFs （CMESFs）, monkey oviductal fibroblasts （MOFs）, monkey follicular granulosa fibroblast-like （MFGs） cells, monkey follicular granulosa epithelium-like （MFGEs） cells, were developed for the maintenance of rhesus embryonic stem cells （rESCs）. We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs, mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs.展开更多
In this paper,an embedded real-time control system for automatic rotor balancing was studied.Benefiting from the modular design,this system can be easily re-constituted or expanded under different working conditions.T...In this paper,an embedded real-time control system for automatic rotor balancing was studied.Benefiting from the modular design,this system can be easily re-constituted or expanded under different working conditions.The special designed hardware resists harsh environment.As an embedded application,it was very important to save system consumptions on both hardware and software,so the algorithms for unbalance vibration identification and attenuation were deduced,meantime a unified fast algorithm structure was achieved through the geometric analysis.Based on this structure,the signal processing algorithm was tested by an open data source,while the control algorithm was simulated using a basic rotor model,and then connected to a hyper gravity machine running online auto-balancing.The result confirms that the unbalancing vibration is effectively restrained.展开更多
The contemporary emphasis on learner-centeredness and autonomy suggests that in some contexts, learners' self-correction of mistakes might be more beneficial for language learning than teacher's direction corr...The contemporary emphasis on learner-centeredness and autonomy suggests that in some contexts, learners' self-correction of mistakes might be more beneficial for language learning than teacher's direction correction. A case study was constructed to discover how effective the target students could self-correct their writing mistakes against the teachers' feedback. The findings may give some insights into the role of self- correction in teaching L2 writing.展开更多
Embryonic stem cells （ESCs） can undergo unlimited self-renewal and maintain pluripotency to differen- tiate into any cell type of the three germ layers. Extensive studies have shown ESC identity is regulated by tran...Embryonic stem cells （ESCs） can undergo unlimited self-renewal and maintain pluripotency to differen- tiate into any cell type of the three germ layers. Extensive studies have shown ESC identity is regulated by transcription factors, epigenetic regulators and multiple signal transduction pathways. However, the kinase regulation of pluripotency is not well understood. Here we show that the serine/threonine kinase PlM2, which is highly expressed in ESCs but not in somatic cells, functions as a crucial stemness regulator in ESCs. Knockout of Pim2 inhibits the self-renewal and differentiation capability of ESCs. Mechanistic studies identified that PIM2 can directly phosphorylate 4E-BP1, leading to release of elF4E which facili- tates the translation of pluripotent genes in ESCs. Our study highlights a novel kinase cascade pathway for ESC identity maintenance.展开更多
文摘In our previous study, five homologous feeder cell lines, Monkey ear skin fibroblasts （MESFs）, clonally derived fibroblasts from the MESFs （CMESFs）, monkey oviductal fibroblasts （MOFs）, monkey follicular granulosa fibroblast-like （MFGs） cells, monkey follicular granulosa epithelium-like （MFGEs） cells, were developed for the maintenance of rhesus embryonic stem cells （rESCs）. We found that MESFs, CMESFs, MOFs and MFGs, but not MFGEs, support the growth of rhesus embryonic stem cells. Moreover, we detected some genes that are upregulated in supportive feeder cell lines by semi-quantitative PCR. In the present study, we applied the GeneChip Rhesus Macaque Genome Array of Affymetrix Corporation to study the expression profiles of these five feeder cell lines, in purpose to find out which cytokines and signaling pathways were important in maintaining the rESCs, mRNAs of eight genes, including GREM2, bFGF, KITLG, DKK3, GREM1, AREG, SERPINF1 and LTBP1, were found to be upregulated in supportive feeder cell lines, but not in MFGE. The results indicate that many signaling pathways may play redundant roles in supporting the undifferentiated growth and maintenance of pluripotency in rESCs.
基金National Natural Science Foundation of China(No.50635010)
文摘In this paper,an embedded real-time control system for automatic rotor balancing was studied.Benefiting from the modular design,this system can be easily re-constituted or expanded under different working conditions.The special designed hardware resists harsh environment.As an embedded application,it was very important to save system consumptions on both hardware and software,so the algorithms for unbalance vibration identification and attenuation were deduced,meantime a unified fast algorithm structure was achieved through the geometric analysis.Based on this structure,the signal processing algorithm was tested by an open data source,while the control algorithm was simulated using a basic rotor model,and then connected to a hyper gravity machine running online auto-balancing.The result confirms that the unbalancing vibration is effectively restrained.
文摘The contemporary emphasis on learner-centeredness and autonomy suggests that in some contexts, learners' self-correction of mistakes might be more beneficial for language learning than teacher's direction correction. A case study was constructed to discover how effective the target students could self-correct their writing mistakes against the teachers' feedback. The findings may give some insights into the role of self- correction in teaching L2 writing.
基金supported by the National Basic Research Program of China (2013CB966901)the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA01040108)The National Thousand of Talents Program to T.Z., and the National Natural Science Foundation of China (31570995, 31621004) to T.Z. and (31400831) to J.C.
文摘Embryonic stem cells （ESCs） can undergo unlimited self-renewal and maintain pluripotency to differen- tiate into any cell type of the three germ layers. Extensive studies have shown ESC identity is regulated by transcription factors, epigenetic regulators and multiple signal transduction pathways. However, the kinase regulation of pluripotency is not well understood. Here we show that the serine/threonine kinase PlM2, which is highly expressed in ESCs but not in somatic cells, functions as a crucial stemness regulator in ESCs. Knockout of Pim2 inhibits the self-renewal and differentiation capability of ESCs. Mechanistic studies identified that PIM2 can directly phosphorylate 4E-BP1, leading to release of elF4E which facili- tates the translation of pluripotent genes in ESCs. Our study highlights a novel kinase cascade pathway for ESC identity maintenance.
