: To study the effect of acupuncture on interleukin 2 (IL 2)-interferon(IFN)-natural killer cells (NKC) immunoregulatory network of mice with transplanted hepatocarcinoma(HAC) in order to provide new evidence for acup...: To study the effect of acupuncture on interleukin 2 (IL 2)-interferon(IFN)-natural killer cells (NKC) immunoregulatory network of mice with transplanted hepatocarcinoma(HAC) in order to provide new evidence for acupuncture treatment of hypofunction of immune system. Methods:The 28 HAC-vaccinated BALB/C mice are randomly divided into control group (n=14) and acupunctrue group (n=14). In the latter group bilateral'Dazhui' (BL 11) and 'Zusanili' (ST 36) which are located according to the same positions indicated in Comparative Anatomy of Macro-animals, are needled once every day, twelve sessions altogether. Twenty-four hours after the needling treatment the mice are killed and the spleen is taken out to be made into cell suspension for assaying concentrations of IL2 (MTT method) and NKC (colorimetric method) respectively. Obital Serum IFN is determined by using (CPE microplate staining), and the tumor mass is taken out and balanced with an analytical balance (1/10000) to calculate the tumor inhibition rate according to the formula. Results: The tumor weight of the mice of aupuncture group is obviously decreased (inhibition rate 43.06%) while the activity of the IL2 and NKC, and the IFN tiler are increased greatly, which have significant differences compared with those of control group (P>0.01). Conclusion:The results of the study show that acupuncture can strengthen the positive immunoregulatory function of the IL2 -IFN-NKC network in immune hypofunction mice bearing HAC.展开更多
Objective： To observe the variation and significance of natural killer T （NKT） cells in patients with severe multiple injuries. Methods： Peripheral blood was drawn from 30 patients with severe multiple injuries a...Objective： To observe the variation and significance of natural killer T （NKT） cells in patients with severe multiple injuries. Methods： Peripheral blood was drawn from 30 patients with severe multiple injuries and 20 healthy individuals. NKT cells and the subsets of NKT cells were stained and analyzed on fluorescence activated cell sorter （FACS） using Cellquest software. The level of IL-4 and IFN- γ in blood serum was detected by ELISA. Results： The proportion of NKT cells was significantly increased. CD4^＋ NKT cells was increased （t=-3.11, P〈0.01） and CD4^＋CD8^＋NKT （double negative NKT, DN NKT） cells decreased in patients with severe multiple injuries compared with healthy controls （t=2.99, P〈0.01）. There was a positive correlation between the proportion of NKT cells and injury severity score （ISS） by Spearman correlation analysis （r=0.70, P〈0.01）. The level of IFN-γ was significantly decreased and the level of IL-4 significantly increased in patients with severe multiple injuries. Conclusions： We demonstrate that human NKT cells are increased in trauma patients. Most significantly, there is an association between ISS and NKT cells. The increased CD4^＋NKT cells may contribute to the reduction of Thl cytokine production and the growth of Th2 cytokine production, leading to the suppression of immunity after injury.展开更多
基金the National Natural Science Fundationa of China(38970885)
文摘: To study the effect of acupuncture on interleukin 2 (IL 2)-interferon(IFN)-natural killer cells (NKC) immunoregulatory network of mice with transplanted hepatocarcinoma(HAC) in order to provide new evidence for acupuncture treatment of hypofunction of immune system. Methods:The 28 HAC-vaccinated BALB/C mice are randomly divided into control group (n=14) and acupunctrue group (n=14). In the latter group bilateral'Dazhui' (BL 11) and 'Zusanili' (ST 36) which are located according to the same positions indicated in Comparative Anatomy of Macro-animals, are needled once every day, twelve sessions altogether. Twenty-four hours after the needling treatment the mice are killed and the spleen is taken out to be made into cell suspension for assaying concentrations of IL2 (MTT method) and NKC (colorimetric method) respectively. Obital Serum IFN is determined by using (CPE microplate staining), and the tumor mass is taken out and balanced with an analytical balance (1/10000) to calculate the tumor inhibition rate according to the formula. Results: The tumor weight of the mice of aupuncture group is obviously decreased (inhibition rate 43.06%) while the activity of the IL2 and NKC, and the IFN tiler are increased greatly, which have significant differences compared with those of control group (P>0.01). Conclusion:The results of the study show that acupuncture can strengthen the positive immunoregulatory function of the IL2 -IFN-NKC network in immune hypofunction mice bearing HAC.
文摘Objective： To observe the variation and significance of natural killer T （NKT） cells in patients with severe multiple injuries. Methods： Peripheral blood was drawn from 30 patients with severe multiple injuries and 20 healthy individuals. NKT cells and the subsets of NKT cells were stained and analyzed on fluorescence activated cell sorter （FACS） using Cellquest software. The level of IL-4 and IFN- γ in blood serum was detected by ELISA. Results： The proportion of NKT cells was significantly increased. CD4^＋ NKT cells was increased （t=-3.11, P〈0.01） and CD4^＋CD8^＋NKT （double negative NKT, DN NKT） cells decreased in patients with severe multiple injuries compared with healthy controls （t=2.99, P〈0.01）. There was a positive correlation between the proportion of NKT cells and injury severity score （ISS） by Spearman correlation analysis （r=0.70, P〈0.01）. The level of IFN-γ was significantly decreased and the level of IL-4 significantly increased in patients with severe multiple injuries. Conclusions： We demonstrate that human NKT cells are increased in trauma patients. Most significantly, there is an association between ISS and NKT cells. The increased CD4^＋NKT cells may contribute to the reduction of Thl cytokine production and the growth of Th2 cytokine production, leading to the suppression of immunity after injury.
