在法医病理学鉴定工作中，常遇到死亡时间较长的组织创伤案件，由于组织中红细胞自溶碎裂后与胶原纤维等混杂．用常规HE组织切片染色难以判别系出血、生前伤或死后伤。本研究借用区别胶原纤维、红细胞和肌肉组织的染色法（Ponceau—Vict...在法医病理学鉴定工作中，常遇到死亡时间较长的组织创伤案件，由于组织中红细胞自溶碎裂后与胶原纤维等混杂．用常规HE组织切片染色难以判别系出血、生前伤或死后伤。本研究借用区别胶原纤维、红细胞和肌肉组织的染色法（Ponceau—Victoria blue B染色，P—V染色法）解决这一难题，取得了良好的效果。展开更多
Macroautophagy is a multistep, vacuolar, degradation pathway terminating in the lysosomal compartment, and it is of fundamental importance in tissue homeostasis. In this review, we consider macroautophagy in the light...Macroautophagy is a multistep, vacuolar, degradation pathway terminating in the lysosomal compartment, and it is of fundamental importance in tissue homeostasis. In this review, we consider macroautophagy in the light of recent advances in our understanding of the formation of autophagosomes, which are double-membrane-bound vacuoles that sequester cytoplasmic cargos and deliver them to lysosomes. In most cases, this final step is preceded by a maturation step during which autophagosomes interact with the endocytic pathway. The discovery of AuTophaGyrelated genes has greatly increased our knowledge about the mechanism responsible for antophagosome formation, and there has also been progress in the understanding of molecular aspects of autophagosome maturation. Finally, the regulation of autophagy is now better understood because of the discovery that the activity of Atg complexes is targeted by protein kinases, and owing to the importance of nuclear regulation via transcription factors in regulating the expression of autophagy genes.展开更多
文摘在法医病理学鉴定工作中，常遇到死亡时间较长的组织创伤案件，由于组织中红细胞自溶碎裂后与胶原纤维等混杂．用常规HE组织切片染色难以判别系出血、生前伤或死后伤。本研究借用区别胶原纤维、红细胞和肌肉组织的染色法（Ponceau—Victoria blue B染色，P—V染色法）解决这一难题，取得了良好的效果。
文摘Macroautophagy is a multistep, vacuolar, degradation pathway terminating in the lysosomal compartment, and it is of fundamental importance in tissue homeostasis. In this review, we consider macroautophagy in the light of recent advances in our understanding of the formation of autophagosomes, which are double-membrane-bound vacuoles that sequester cytoplasmic cargos and deliver them to lysosomes. In most cases, this final step is preceded by a maturation step during which autophagosomes interact with the endocytic pathway. The discovery of AuTophaGyrelated genes has greatly increased our knowledge about the mechanism responsible for antophagosome formation, and there has also been progress in the understanding of molecular aspects of autophagosome maturation. Finally, the regulation of autophagy is now better understood because of the discovery that the activity of Atg complexes is targeted by protein kinases, and owing to the importance of nuclear regulation via transcription factors in regulating the expression of autophagy genes.
