Objective: We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia. Study design: We studied prospectively 146 patients wi...Objective: We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia. Study design: We studied prospectively 146 patients with ≥3 consecutive early, late, or early-late recurrent pregnancy losses, together with 99 age-matched controls. Anticardiolipin antibodies (ACL), lupus anticoagulant (LA), and APC resistance (APCR) were detected by ELISA, dilute Russell Viper Venom Time (dRVVT), and coagulation tests, respectively, and FV-Leiden and PRT G20210A genotypes were assessed by PCR. Results: Anti-PL antibody frequencies were 45%and 9%among patients and controls, respectively (P < 0.001), with positive LA only (P = 0.004), or combined elevated ACL-positive LA being consistently higher (P < 0.001) among patients than controls. FV-Leiden (20.54%versus 6.06%), but not PRT G20210A (2.74%versus 4.04%) was significantly higher in patients versus controls. Among LA-positive cases higher prevalence of G/A (14/146 versus 1/99) and A/A genotypes (4/146 versus 0/99) were seen, and among ACL-positive cases higher prevalence of G/A (10/146 versus 0/99) and A/A genotypes (2/146 versus 0/99) were recorded. Conclusions: Anti-PL antibodies and FV-Leiden, but not PRT G20210A, are associated with recurrent idiopathic pregnancy losses in Tunisian women.展开更多
The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets ...The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets were analyzed for an adenine (A) to guanine (G) substitution at position - 670 in the TNFRSF6 promoter. Pregnancy outcome data were subsequently obtained. Analysis was by Fisher exact test. Maternal allele G homozygosity (TNFRSF6* G) was observed in 42.4% of 33 PPROM pregnancies as opposed to 19.5% of 77 with no spontaneous preterm birth (P =. 01). Similarly, TNFRSF6* G homozygosity was present in 37.5% of 32 first- born neonates from PPROM pregnancies as opposed to 18.7% of 75 uncomplicated pregnancies (P =. 04). PPROM occurred in 8 of 14 (57.1% ) pregnancies in which mother and all neonates were TNFRSF6* G homozygotes as opposed to 25 of 105 (23.8% )- cases in which uniform TNFRSF6* G homozygosity was not observed (P =. 02). A genetic variant in the Fas gene is associated with an increased rate of PPROM in multifetal pregnancies.展开更多
文摘Objective: We assessed the prevalence of inherited (FV-Leiden and PRT G20210A), and acquired (anti-PL antibodies) risk factors among habitual aborters in Tunisia. Study design: We studied prospectively 146 patients with ≥3 consecutive early, late, or early-late recurrent pregnancy losses, together with 99 age-matched controls. Anticardiolipin antibodies (ACL), lupus anticoagulant (LA), and APC resistance (APCR) were detected by ELISA, dilute Russell Viper Venom Time (dRVVT), and coagulation tests, respectively, and FV-Leiden and PRT G20210A genotypes were assessed by PCR. Results: Anti-PL antibody frequencies were 45%and 9%among patients and controls, respectively (P < 0.001), with positive LA only (P = 0.004), or combined elevated ACL-positive LA being consistently higher (P < 0.001) among patients than controls. FV-Leiden (20.54%versus 6.06%), but not PRT G20210A (2.74%versus 4.04%) was significantly higher in patients versus controls. Among LA-positive cases higher prevalence of G/A (14/146 versus 1/99) and A/A genotypes (4/146 versus 0/99) were seen, and among ACL-positive cases higher prevalence of G/A (10/146 versus 0/99) and A/A genotypes (2/146 versus 0/99) were recorded. Conclusions: Anti-PL antibodies and FV-Leiden, but not PRT G20210A, are associated with recurrent idiopathic pregnancy losses in Tunisian women.
文摘The relationship between a polymorphism at position - 670 in the Fas gene (TNFRSF6) and preterm premature rupture of membranes (PPROM) in multifetal pregnancies was examined. Buccal swabs from 119 mother- infant sets were analyzed for an adenine (A) to guanine (G) substitution at position - 670 in the TNFRSF6 promoter. Pregnancy outcome data were subsequently obtained. Analysis was by Fisher exact test. Maternal allele G homozygosity (TNFRSF6* G) was observed in 42.4% of 33 PPROM pregnancies as opposed to 19.5% of 77 with no spontaneous preterm birth (P =. 01). Similarly, TNFRSF6* G homozygosity was present in 37.5% of 32 first- born neonates from PPROM pregnancies as opposed to 18.7% of 75 uncomplicated pregnancies (P =. 04). PPROM occurred in 8 of 14 (57.1% ) pregnancies in which mother and all neonates were TNFRSF6* G homozygotes as opposed to 25 of 105 (23.8% )- cases in which uniform TNFRSF6* G homozygosity was not observed (P =. 02). A genetic variant in the Fas gene is associated with an increased rate of PPROM in multifetal pregnancies.
