Random Amplified Polymorphic DNA (RAPD) analysis is a new technology of molecular marking which has proved very powerful in detecting genetic diversity at the level of population. The genomic DNAs used in our experime...Random Amplified Polymorphic DNA (RAPD) analysis is a new technology of molecular marking which has proved very powerful in detecting genetic diversity at the level of population. The genomic DNAs used in our experiment were extracted from fresh leaves taken from 59 individuals sampled from three natural populations in Yan An, Shanxi Province. Through more than 2,000 PCRs, deep-going RAPD analysis was carried out on DNA samples from 49 inviduals. The percentage of polymorphic RAPD loci found in these three populations were respectively 27.2 %, 18.6 % and 5.4 %; the average genetic distances within population, 0.055, 0.036 and 0.008; the average genetic distances between populations (Ⅰ-Ⅱ), (Ⅰ-Ⅲ) and (Ⅱ-Ⅲ), 0.105, 0.096 and 0.060. The genetic diversity of A. brachypus within and between populations was found, for the first time, to be rather poor,thus revealing innate factors as the cause contributing to its endangered status. In addition, our work also provides basic materials for elucidating the underlying cause of its endangerment and for its protection biology.展开更多
In this study, the quadrat method was used to study the effects of tsaoko （Fructus tsaoko） plantation on tree diversity and canopy structure of two natural habitats of eastern hoolock gibbon （Hoolock leuconedys）：...In this study, the quadrat method was used to study the effects of tsaoko （Fructus tsaoko） plantation on tree diversity and canopy structure of two natural habitats of eastern hoolock gibbon （Hoolock leuconedys）： Nankang （characterized by extensive tsaoko plantation） and Banchang （relatively well reserved and without tsaoko plantation）. Totally, 102 tree species from 25 families and 16 woody liana species from 10 families were recorded in Nankang, whereas 108 tree species from 30 families and 17 woody liana species from 12 families were recorded in Banchang. Although the tree species between two habitats is different, both habitats are characterized by enriched food resources for eastern hoolock gibbons, sharing similar dominant plant families. Due to tsaoko plantation, tree density proportion and diversity of forest layer I （〉20 m） in Nankang were both significantly decreased, but the tree density of layerH （10-20 m） increased. Likewise, in conjunction with these behavioral observations, we also address potential impacts of tsaoko plantation on the behavior of eastern hoolock gibbon.展开更多
In order to understand the pattern and status of plant species in the forest-meadow ecotonein Sanjiangyuan Nature Reserve, this study was undertaken through expert consultation, literaturesurvey and fieldwork in vario...In order to understand the pattern and status of plant species in the forest-meadow ecotonein Sanjiangyuan Nature Reserve, this study was undertaken through expert consultation, literaturesurvey and fieldwork in various parts of the ecotone during 2002-2004. Based on 3 important systems,i.e. threatened status, genetic loss effect and use value, the list of threatened and prioritized plants wasestablished. In this paper, the threatened coefficient was defined as the criteria of category for threatenedplants, the conservation priorities coefficient summed by threatened coefficient, genetic coefficient anduse value coefficient was defined as the criteria of prioritized plants. In addition, 10 indicators toevaluate threatened grading and conservation priorities sequence were included in the 3 importantsystems. The weights of 3 systems and 10 indicators were given through expert consultation andanalytic hierarchy process. Ten indicators were given scores based on the subcriteria, respectively, andall the scores of the 3 systems were summed up for each species, then contrasted to the criteria ofthreatened grading and conservation priorities for plant species. The status of endangerment andconservation priorities of plant species were analyzed, and the results showed that the number ofendangered species was 2, vulnerable species 37, near threatened species 140, safe species 767; the firstconcern category had 4 species, the second concern category 18, the third concern category 150 and theleast concern category 774.展开更多
文摘Random Amplified Polymorphic DNA (RAPD) analysis is a new technology of molecular marking which has proved very powerful in detecting genetic diversity at the level of population. The genomic DNAs used in our experiment were extracted from fresh leaves taken from 59 individuals sampled from three natural populations in Yan An, Shanxi Province. Through more than 2,000 PCRs, deep-going RAPD analysis was carried out on DNA samples from 49 inviduals. The percentage of polymorphic RAPD loci found in these three populations were respectively 27.2 %, 18.6 % and 5.4 %; the average genetic distances within population, 0.055, 0.036 and 0.008; the average genetic distances between populations (Ⅰ-Ⅱ), (Ⅰ-Ⅲ) and (Ⅱ-Ⅲ), 0.105, 0.096 and 0.060. The genetic diversity of A. brachypus within and between populations was found, for the first time, to be rather poor,thus revealing innate factors as the cause contributing to its endangered status. In addition, our work also provides basic materials for elucidating the underlying cause of its endangerment and for its protection biology.
基金supported by the National Natural Science Foundation of China(31160424)Natural Science Foundation of Yunnan Province(20110426)Science Foundation Project of Mt.Gaoligong National Natural Reserve(201215)
文摘In this study, the quadrat method was used to study the effects of tsaoko （Fructus tsaoko） plantation on tree diversity and canopy structure of two natural habitats of eastern hoolock gibbon （Hoolock leuconedys）： Nankang （characterized by extensive tsaoko plantation） and Banchang （relatively well reserved and without tsaoko plantation）. Totally, 102 tree species from 25 families and 16 woody liana species from 10 families were recorded in Nankang, whereas 108 tree species from 30 families and 17 woody liana species from 12 families were recorded in Banchang. Although the tree species between two habitats is different, both habitats are characterized by enriched food resources for eastern hoolock gibbons, sharing similar dominant plant families. Due to tsaoko plantation, tree density proportion and diversity of forest layer I （〉20 m） in Nankang were both significantly decreased, but the tree density of layerH （10-20 m） increased. Likewise, in conjunction with these behavioral observations, we also address potential impacts of tsaoko plantation on the behavior of eastern hoolock gibbon.
基金This research is one part of "The Rapid Evaluation Technology of Threatened Species in Sanjiangyuan Nature Reserve " (2001BA510B10-03), which is supported by the National Advanced Project of the Tenth Five-year Plan.
文摘In order to understand the pattern and status of plant species in the forest-meadow ecotonein Sanjiangyuan Nature Reserve, this study was undertaken through expert consultation, literaturesurvey and fieldwork in various parts of the ecotone during 2002-2004. Based on 3 important systems,i.e. threatened status, genetic loss effect and use value, the list of threatened and prioritized plants wasestablished. In this paper, the threatened coefficient was defined as the criteria of category for threatenedplants, the conservation priorities coefficient summed by threatened coefficient, genetic coefficient anduse value coefficient was defined as the criteria of prioritized plants. In addition, 10 indicators toevaluate threatened grading and conservation priorities sequence were included in the 3 importantsystems. The weights of 3 systems and 10 indicators were given through expert consultation andanalytic hierarchy process. Ten indicators were given scores based on the subcriteria, respectively, andall the scores of the 3 systems were summed up for each species, then contrasted to the criteria ofthreatened grading and conservation priorities for plant species. The status of endangerment andconservation priorities of plant species were analyzed, and the results showed that the number ofendangered species was 2, vulnerable species 37, near threatened species 140, safe species 767; the firstconcern category had 4 species, the second concern category 18, the third concern category 150 and theleast concern category 774.
