从“抒情诗”到“普罗”:1920年代末戏剧的自白体对话问题

1920年代末是中国现代戏剧史的一个独特时期,不仅创作数量上高产,而且仅两三年,戏剧就完成了从"抒情诗"到"普罗"的转变,这和戏剧对话上的自白体现象密切相关,它既表明剧人对"自我"真理的偏执,也在一定条件下极易走向戏剧的反面,它决定了田汉等人创作转向的必然。实际上,自白体对话问题反映出这时期剧人在戏剧观念上的本土选择。

自我与超越自我的神话——美国自白体诗歌的双重维度

上世纪60年代初,美国自白体诗歌在文坛异军突起。自白体诗歌并不是诗人单纯的自我倾诉。作为美国后现代主义诗歌中的一个重要流派,自白派诗人通过面向公众的自白,来揭示那一个时代美国人的生活环境与精神状态,所以,美国自白体诗歌具有了自我与社会的双重维度的性质。本文试图通过对自白体诗歌的内核与外延的分析,把握自白体诗歌所具有的双重维度,重新挖掘自白体诗歌对于人类生活状态所给予的人文关注。

论卡玛拉·达斯自白体诗歌的女性主义意义

卡玛拉·达斯是当代印度著名的女诗人之一,她以直率大胆的诗风在文坛独树一帜。其诗歌以自白的方式书写了自我私密性的生活,表现了女性丰富的情感世界和细腻的心理以及在两性生活中的痛苦体验,呈现出女性觉醒的历程。其诗歌具有强烈的女性意识,由女性面对痛苦的自我拯救走向对印度文化男性霸权的批判,具有普遍的女性主义意义。

美国自白派诗歌的狂欢化写作

自白派诗歌是美国二十世纪六十年代特有的文学现象。文章通过金斯伯格、普拉斯、贝德曼、赛克斯顿等自白派代表诗人的作品,剖析自白派诗歌的特点,从而进一步阐述自白派诗歌的狂欢化写作。文章发现,自白体诗歌是诗人为了挣脱自身痛苦经历的个人化与狂欢化写作,在一定程度上它反映了诗人的生活,但是由于自白体诗歌中上帝的缺场以及对于宏大史实的书写,使得自白体诗歌高于自身经验,成为信仰缺失和真实世界的隐喻。

Association of primary biliary cirrhosis with idiopathic thrombocytopenic purpura

Although both primary biliary cirrhosis (PBC) and idiopathic thrombocytopenic purpura (ITP) are autoimmune diseases, the association of the 2 diseases is rare. Here, we report a case of ITP that developed during the follow-up of PBC in a 74-year- old man. The patient had been diagnosed with PBC 12 years previously, and had received treatment with ursodeoxycholic acid. The platelet count decreased from approximately 60 × 109/L to 8 × 109/L, and the association of decompensated liver cirrhosis (PBC) with ITP was diagnosed. Steroid and immune gamma globulin therapy were successful in increasing the platelet count. Interestingly, human leukocyte antigen genotyping detected the alleles DQB10601 and DRB10803, which are related to both PBC and ITP in Japanese patients. This case suggests common immunogenetic factors might be involved in the development of PBC and ITP.

Genetic association of autoimmune hepatitis and human leucocyte antigen in German patients

AIM: To report on our large German collective and updated data of 142 patients with autoimmune hepatitis (AIH) type 1. METHODS: Key investigations performed were liver biopsy, serum autoantibodies as well as serum markers such as IgG and elevated transaminases. Antinuclear antigen (ANA) and smooth muscle antigen (SMA) autoantibodies characterized type 1 AIH. Type 3 (AIH) was solely characterized by the occurrence of soluble liver antigen/liver-pancreas antigen (SLA/LP) autoantibodies either with or without ANA or SMA autoantibodies. RESULTS: Most prevalent HLAs were A2 (68 patients, 48%), B8 (63 patients, 44%), C7 (90 patients, 63%), DR3 (49 patients, 38%), DR4 (49 patients, 38%) and DQ2 (42 patients, 30%). Compared to the Italian and North American patients, we found fewer patients with a DQ2 subtype. Furthermore, the B8-DR3-DQ2 human leucocyte antigen (HLA) was also less prominent compared to the North American patients. However, prevalences of B8, DR3, DR4, DR7, DR11 and DR13 were comparable to the Italian and North American patients. Furthermore, we report on an additional subgroup of patients with SLA/LP positive AIH. Generally, in this subgroup of patients the same HLA subtypes were favoured as the AIH type 1. CONCLUSION: Although HLA subtypes were comparable between these three collectives, the German patients were distinct from the Italian and North American patients with respect to DQ2 and from the North American patients with respect to B8-DR3-DQ2HLA. A clinical correlation, e.g. difference in severity or treatability of AIH type 1, has yet to be determined.

EFFECTS OF ANTICOAGULATION PROTEIN DEFECT IN MATERNAL PLASMA ON SPONTANEOUS ABORTION

Objective To investigate the mechanism of anticoagulation protein defect in the pathogenesis of unexplained recurrent miscarriage. Methods Fifty-seven patients with a history of unexplained abortion were enrolled as the investigation group for tests of protein C, protein S, antithrombinⅢ(AT-Ⅲ), as well as activated protein C resistance (APC-R). The control group con-sisted of fifty healthy women with a history of normal pregnancy and delivery. Blood samples were obtained for measuring serum activity of protein C, protein S, AT-Ⅲ, and APC-R. Patients with positive APC-R were tested for factorⅤ(FⅤ) Lei-den gene mutation by PCR-RFLP method. Results Of the 57 patients, 12 (21.1%), 1 (1.8%), and 5 (8.8%) cases were found with protein S, protein C, and AT-Ⅲdeficiency respectively, and 13 (22.8%) cases with positive results of APC-R. Of the control group, no protein C or AT-Ⅲdeficiency was ever found, whereas 2 (4.0%) volunteers were presented with protein S deficiency and 3 (6.0%) with positive results of APC-R. No FⅤLeiden gene mutation was identified in all the patients with positive APC-R results. Late spontan-eous abortion cases had higher incidence of anticoagulation protein defect than the early cases. Conclusion Anticoagulation protein defect may play a role in the pathogenesis of fetal loss, especially for those occurr-ing in late stage of pregnancy.

Screening for celiac disease in Down's syndrome patients revealed cases of subtotal villous atrophy without typical for celiac disease HLA-DQ and tissue transglutaminase antibodies

A1M： To investigate the prevalence of celiac disease （CD） as well as CD marker antibodies and susceptibility HLA-DQ haplotypes in 134 karyotyped Down＇s syndrome （DS） patients. METHODS： Immunoglobulin A （IgA） and G （IgG） type anti-gliadin antibodies （AGA）, IgA type anti-tissue transglutaminase （tTG） antibodies （anti-tTG） with antigen of guinea pig and human source were determined by enzyme-linked immunosorbent assay and endomysium antibodies （EHA） by indirect immunofiuoresence test. HLA-DQA1＊0501/DQB1＊0201 （DQ2） was revealed by polymerase chain reaction. Celiac disease was diagnosed by revised ESPGHAN criteria. RESULTS： 41% of DS patients had AGA, 6.0% IgA anti-tTG with guinea pig antigen, and 3.0 % [gA EMA （all positive for anti-tTG with human tTG）. Subtotal villous atrophy was found in 5 out of 9 DS patients who had agreed to small bowel biopsy. One of them had DQA1＊0S01/DQB1＊0201 and anti-tTG and EMA i.e. typical for CD markers （this case also fulfilled the ESPGHAN diagnostic criteria）, but other four lacked these markers. Three non-biopsied DS patients had also most probably CD because DQA1＊0S01/DQB1＊0201 and IgA anti-tTG （EMA） were detected. Thus, the prevalence of CD among our DS patients population is 3.0 % （95 % of confidence interval [CI]： 0.1-5.9 %）. CONCLUSION： We confirm the increased frequency of CD among DS patients. In addition, we have revealed a subgroup of patients with subtotal villous atrophy but without characteristic for CD immunological and genetic markers. Whether these cases represent CD （with atypical immunopathogenesis） or some other immune enteropathy, requires further investigations.

Influence of anti-keratin autoantibodies on telomerase activity of squa-mous cell carcinoma

Objective: To investigate the influence of anti-keratin autoantibodies (AK auto Abs) on telom-erase activity of squamous cell carcinoma cultured in vitro and the mechanisms of the inhibitory effects of AK auto Abs on squamous cell carcinoma. Methods: Influence of AK auto Abs on the proliferation of Tca cells was observed by MTT colorimetry. Telomerase activity of cultured Tca cells and human keratinocytes was determined by telomeric repeat amplication protocol-ELISA (TRAP-ELISA) and polyacrylamide gel elec-trophoresis (PAGE). After being treated with AK auto Abs for 36 h at a concentration of 4, 8, 16 mg/L respectively, the changes of telomerase activity of Tea cells were also detected by TRAP-ELISA and PAGE. Results: MTT colorimetric determination showed that the capacity of proliferation of Tca cells correlated negatively with the concentration of AK auto Abs (r= -0. 74, P<0. 01). TRAP-ELISA and PAGE showed that telomerase activity of Tca cells increased significantly compared to that of cultured human keratinocytes (t = 3. 5396, P<0. 01). AK auto Abs at a concentrations of 4, 8, 16 mg/L had significant dose-dependent inhibitory effects on telomerase activity of Tca cells (r= - 0. 8358, P<0. 01). Conclusion: AK auto Abs have a significant dose-dependent inhibitory effect on the proliferation of cultured Tea cells. AK auto Abs inhibit telomerase activity of cultured Tca cells with dose-dependent pattern. It suggests that decrease of telomerase activity may play an important role in the inhibitory effects of AK auto Abs on squamous cell carcinoma.

Purification and identification of natural antikeratin autoantibody from normal human sera and its effects on the cultured keratinocytes

Objective: To purify the natural antikeratin autoantibody (AK auto-Ab) and observe its effects on the prolif eration of the cultured keratinocytes. Methods: Natural AK auto-Ab was purified by using keratin affinity column, and then the titre and specificity of the Abs were studied by ELISA, immunoperoxidase staining and immuno-electronicmicroscope. The effect of the purified Abs on the cultured keratino-cytes was assayed by 3H-TdR incorporation. Results: Natural AK auto-Ab was obtained. The binding activity of IgG AK auto-Ab in purified Ah remained similar to that in pooled human sera. and the specificity of the obtained antibody is strong. The purified antibody could decrease the Il-TdR incorporation of the cultured keratinocytes in a dose-dependent manner. Conclusion: The method of punning AK auto-Ab is simple, practicable and reliable. Natural AK auto-Ab, existing in normal human individuals, has inhibitory etiect on the proliferation of the cultured keratinocytes.

QTL Analysis of Chalkiness in Rice

A population of 150 recombination inbred lines （RILs） derived from the cross between rice varieties V20B and CPSLO17, was applied to locate the QTLs related to chalkiness traits and evaluate their genetic effects. A genetic linkage map was constructed based on 8 602 SLAF （specific-locus amplified fragment） markers, combine with the chatkiness traits of the tested lines. Four QTLs that related to chalkiness were detected using MapQTL 5 software, named qC-5a, qC-5b, qC-5c and qC-5d. The LOD threshold values of qC-5a, qC-5b, qC-5c and qC-5d were 4.02, 4.09, 3.94 and 4.1, respectively, explaining 11.6%, 11.8%, 11.2% and 11.8% of the observed phenotypic variance. All the four detected QTL alleles came from Iow-chalkiness parent V20B.

Population Bursts of Parvalbumin-Positive Interneurons Inhibit Spiking Pyramidal Cells in Spontaneously Active Cortical in Vitro Networks

Author present the interplay between different neuron types in the spontaneous electrical activity of low density cortical in vitro networks grown on MEA （multielectrode arrays） of glass neurochips. In 10% of the networks, the continuously spiking activity of some neurons was inhibited by synchronous bursts or superbursts of the majority of the other neurons. Immunohistochemical staining subsequent to MEA recordings suggest that the synchronously bursting neurons are parvalbumin-positive interneurons with abundant axonal ramifications. Blocking chemical synaptic transmission by Ca2＋-free medium revealed that the curbed spiking neurons are intrinsically active. It is assumed that these neurons are pyramidal cells which may be inhibited by groups of synchronously bursting interneurons. It is propose that the observed burst-induced inhibition is an important principle in the temporal organization of neuronal activity as well as in the restriction of excitation, and thus essential for information processing in the cerebral cortex.

Population sizes and group characteristics of Siberian Crane(Leucogeranus leucogeranus) and Hooded Crane(Grus monacha) in Poyang Lake Wetland

Both the Siberian Crane(Leucogeranus leucogeranus) and Hooded Crane(Grus monacha) have limited population sizes and are considered endangered by domestic Chinese and international agencies.To document the current size of their respective populations and characterize their groups,between October 2012 and April 2013 we undertook fieldwork at four nature reserve areas within the Poyang Lake wetlands.We divided Poyanghu National Nature Reserve(PYH) into the Wucheng(PWC) and Hengfeng areas(PHF),because each are each located in different counties.Our fieldwork showed that the Siberian Crane occurred mainly in PYH(364 in the PHF,158 in the PWC) and the Nanjishan Wetland National Nature Reserve(NJS,with 200 individuals).The Hooded Crane was mainly distributed in PYH(302 in the PHF and 154 in the PWC).Family groups accounted for more than 50% of the total number of groups among both species,with Hooded Cranes forming more family groups than Siberian Cranes.Typically,these groups were formed of two adults with one offspring(Siberian Crane),and two adults with two offspring(Hooded Crane),with the mean family group size of the Siberian Crane and Hooded Crane being respectively 2.65±0.53(n=43) and 3.09±0.86(n=47) individuals per group.The mean collective group size of the Siberian Crane and Hooded Crane included 28.09±24.94(n=23) and 28.94±27.97(n=16) individuals per group,respectively,with the proportion of juveniles among Hooded Cranes being more than double that seen among the Siberian Cranes.

Effects of free radicals and amyloid β protein on the currents of expressed rat receptors in Xenopus oocytes

Objective To investigate the effects of free radicals (FRs) and amyloid β protein 1 40 (Aβ 1 40 ) on the functions of expressed neurotransmitter receptors (NRs) in Xenopus oocytes Methods Total RNA and messenger RNA (mRNA) was prepared from 3 month old Wistar rat brain tissues with Promega kits and microinjected into maturated Xenopus oocytes (stages Ⅴ Ⅵ) with 50?nl (50?ng) for each oocyte The microinjected oocytes were incubated with modified Bath's solution at 19 0℃±1 0℃ for receptor expression and their currents were recorded with double electrode voltage clamp technique Superoxide anion free radicals (SAFRs) were produced via a reaction system (HPX/XO) with hypoxanthine (HPX, 0 05?mol/L) and xanthine oxidase (XO, 0 1?U/L) In order to observe the effects of Aβ and SAFRs on the expressed glutamate receptor, HPX/XO and Aβ 1 40 were added to incubation solution at 12?h, 24?h and 96?h before recording Results The results showed that the oocytes expressed functional NRs originating from rat brain tissues These NRs included muscarinic acetylcholine (mACh), glutamate (Glu), dopamine (DA), serotonin (5 HT) and γ aminobutyric acid (GABA) The current characteristics of expressed receptors were inward currents carried by chloride ion with their equibrilium potentials close to -22?mV The extent of effect on the current of expressed glutamate receptor from rat brain was different among different Aβ concentrations and incubation times Aβ 1 40 at a concentration of 20?nmol/L had little effect on the currents of expressed rat brain glutamate receptors up to 24?h of incubation period; but the currents of glutamate receptor were significantly decreased (25% off, P <0 01) in the treatment of 60?nmol/L Aβ 1 40 over 24?h Moreover, when 20?nmol/L Aβ 1 40 was co incubated over 12?h with SAFRs produced by the reaction system of HPX/XO, it was found that the currents of expressed rat brain glutamate receptors had been changed markedly When the oocytes were co treated with 60?nmol/L Aβ 1 40 and SAFRs over a period of 12?h, the currents of glutamate receptor significantly decreased (21% off, P <0 05), and the decreased percentage reached 52% over 24?h co treatment with 60?nmol/L Aβ 1 40 and SAFRs In addition, vitamin E had a partial effect against this inhibitory effect Conclusion The results suggest that Aβ has a kind of inhibitory effect upon the current of the glutamate receptor, similar to the effects of free radicals The effects can be antagonized by vitamin E These imply that Aβ may play a role via inhibiting receptor function in the pathophysiology of Alzheimer's disease