On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Bea...On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Beam Reflectance Measurement (FBRM) technique.The experimental results showed go od correlation between the total counts of the FBRM chord length and the biomass concentration of SFYCPs, which could be used to measure the biomass concentrati on for the SFYCP system quantitatively.Furthermore, the mode of FBRM chord lengt h distribution was linearly correlated to both the mean (R2=0.986) and th e 95th percentile (R2=0.988)of the length-weighted chord length distribu tion, which could be used to evaluate SFYCP size distribution effectively.The CO 2 bubbles produced during cell growth/fermentation were observed and proved to create disturbance on on-line measurement of the SFYCP system.This disturbance could be excluded by estimating SFYCP size distribution based on the mode of FB RM chord length distribution.展开更多
A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was esta...A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was established and continuously operated for 40 days A self flocculating fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe (SPSC 01) was used and ethanol and yeast were co produced from corn dry milling, two stage enzymatic hydrolysis (raw material residues were separated after saccharification).The results showed that average levels of 98 g·L -1 for ethanol, 4 g·L -1 for residual reduced sugar and 6—9 g·L -1 for residual total sugar were achieved when the medium containing total sugar of 220 g·L -1 was fed at a dilution rate of 0 05 h -1 .Pure yeast, equivalent to 5% (mass) of ethanol and containing protein as high as 48 6%(mass), was co produced Ethanol production is expected to be more economically competitive as this high quality yeast potentially can be further processed as value added by products.展开更多
文摘On-line monitoring of self-flocculating yeast cell p articles (SFYCPs) that were characterized by their weak strength, irregular morp hology, special size distribution, and so on, was realized by using the Focused Beam Reflectance Measurement (FBRM) technique.The experimental results showed go od correlation between the total counts of the FBRM chord length and the biomass concentration of SFYCPs, which could be used to measure the biomass concentrati on for the SFYCP system quantitatively.Furthermore, the mode of FBRM chord lengt h distribution was linearly correlated to both the mean (R2=0.986) and th e 95th percentile (R2=0.988)of the length-weighted chord length distribu tion, which could be used to evaluate SFYCP size distribution effectively.The CO 2 bubbles produced during cell growth/fermentation were observed and proved to create disturbance on on-line measurement of the SFYCP system.This disturbance could be excluded by estimating SFYCP size distribution based on the mode of FB RM chord length distribution.
文摘A bioreactor system with a total working volume of 4 8 L,composed of four airlift suspended bed bioreactors, one of which was designated for seed cultivation and the others in series for ethanol fermentation, was established and continuously operated for 40 days A self flocculating fusant from Saccharomyces cerevisiae and Schizosaccharomyces pombe (SPSC 01) was used and ethanol and yeast were co produced from corn dry milling, two stage enzymatic hydrolysis (raw material residues were separated after saccharification).The results showed that average levels of 98 g·L -1 for ethanol, 4 g·L -1 for residual reduced sugar and 6—9 g·L -1 for residual total sugar were achieved when the medium containing total sugar of 220 g·L -1 was fed at a dilution rate of 0 05 h -1 .Pure yeast, equivalent to 5% (mass) of ethanol and containing protein as high as 48 6%(mass), was co produced Ethanol production is expected to be more economically competitive as this high quality yeast potentially can be further processed as value added by products.
