[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom s...[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom strains.[Method] Selecting Agrocybe salicacola strain YAASM0711 as the self-crossing material,six self-crossed combinations were directionally constructed with single spore growth rate as an indicator,to carry out comprehensive analysis of the qualitative traits（color,deformity and growth characteristics）,quantitative traits（shape,yield,size and number of fruit bodies） and mating types of its self-crossed F1 progenies.[Result] After mating factor recombination,self-crossed single spore progenies showed serious genetic differentiation,the average mycelial growth rate and agronomic traits of inbred progenies were lower than those of the parents.The phenotype in fruiting body formation could be divided into normal fruiting and abnormal fruiting（including abnormal fruiting bodies,sporeless or low sporing fruiting bodies,no fruiting）.To be specific,18% self-crossed strains had superior agronomic traits to the parents,which can be used for selecting predominant strains from self-crossed progeny populations based on different breeding purposes.[Conclusion] The genes controlling mycelial growth rate of single spore were linked with mating factors.The mating factor-recombinant single spore strains could be selected according to the correlation between mating type and growth rate.The fruiting result of self-crossed strains indicated that the genes related to sporeless/low sporing development were recessive.Not only genetic materials harboring development-deficient genes in edible mushroom can be obtained by self-crossing,but also the sporeless/low sporing mutant strains with important application prospects can be bred.展开更多
Allelochemicals of Chinese-fir root was extracted by technology ofsupercritical CO_2 extraction under orthogonal experiment design, and it was used to analyzeallelopathic activity of Chinese-fir through bioassay of se...Allelochemicals of Chinese-fir root was extracted by technology ofsupercritical CO_2 extraction under orthogonal experiment design, and it was used to analyzeallelopathic activity of Chinese-fir through bioassay of seed germination. The results showed thatas to the available rate of allelochemicals, the pressure and temperature of extraction were themost important factors. The allelochemicals of Chinese-fir root extracted by pure CO_2 and ethanolmixed with CO_2 have different allelopathic activities to seed germination, and the allelochemicalsextracted by ethanol mixed with CO_2 had stronger inhibitory effects on seed germination than thatextracted by pure CO_2.展开更多
In order to improve the design and implementation quality of web service compositions,formal methods are used to model them and certain properties are verified.WCFA （web service interface control flow automata）is us...In order to improve the design and implementation quality of web service compositions,formal methods are used to model them and certain properties are verified.WCFA （web service interface control flow automata）is used to model web services,especially the control flow and possible interactions with other web services.A web service composition consists of a set of interacting WCFA.The global behavior of web service compositions is captured by NWA（nested word automata）.A variation of the depth-first search algorithm is used to transform a set of WCFA into an NWA.State formulae and call stacks at each node of NWA are computed by a path-sensitive reachability analysis.Safety properties,call stack inspection properties and pre/post-conditions of service invocations are described by assertions.Then verification of these assertions is carried out by an automated SAT tool.展开更多
To realize automatic control of automobile transmission performance test stand Methods The automatic control technique of the lubricant temperature,the program- controll- edautomaticshifting of the transmission,the c...To realize automatic control of automobile transmission performance test stand Methods The automatic control technique of the lubricant temperature,the program- controll- edautomaticshifting of the transmission,the continuous adjusting of revolution speed and load, data-acquisition and data real-time processing were adopted.Results The lubricant temperature was controlled at the set temperature ±2℃.The automatic shifting of the trans- mission is simple,reliable and accurate.The automatic adjusting of load and rotation speed is rapidandaccurate,the torque divergence is ±1N·m,the rotation speed divergence is ±5r/min Conclusion The four kinds of techniques are applied into the automobile transmission perfor- mance test stand successfully. mancetest stand successfully.展开更多
The coal of Anyuan Mine has the characteristic of easy spontaneous combustion. Conventional method is difficult to predict it. Coal samples from this mine were tested in laboratory. The data obtained from laboratory d...The coal of Anyuan Mine has the characteristic of easy spontaneous combustion. Conventional method is difficult to predict it. Coal samples from this mine were tested in laboratory. The data obtained from laboratory determination were initialized for the value which was defined as "K". The ratio of each index gas and value of "K", and the ratio of combination index gases and value of "K", were analyzed simultaneously. The research results show that for this coal mine, if there is carbon monoxide in the gas sample, the phenomenon of oxidation and temperature rising for coal exists in this mine; if there is C_2H_4 in the gas sample, the temperature of coal perhaps exceeds 130 °C. If the coal temperature is between 35 °C and 130 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(CO)/K mainly; if the temperature of coal is between 130 °C and 300 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(C_2H_6)/Φ(C_2H_2) and Φ(C_2H_6)/K. The research results provide experimental basis for the prediction of coal spontaneous combustion in Anyuan coal mine, and have better guidance on safe production of this coal mine.展开更多
Signed direct graph (SDG) theory provides algorithms and methods that can be applied directly to chemical process modeling and analysis to validate simulation models, and is a basis for the development of a software e...Signed direct graph (SDG) theory provides algorithms and methods that can be applied directly to chemical process modeling and analysis to validate simulation models, and is a basis for the development of a software environment that can automate the validation activity. This paper is concentrated on the pretreatment of the model validation. We use the validation scenarios and standard sequences generated by well-established SDG model to validate the trends fitted from the simulation model. The results are helpful to find potential problems, assess possible bugs in the simulation model and solve the problem effectively. A case study on a simulation model of boiler is presented to demonstrate the effectiveness of this method.展开更多
基金Supported by China Agricultural Research System(CARS-24)Natural Science Foundation of Yunnan Province(2011FZ214)National Natural Science Foundation of China(31101591)~~
文摘[Objective] This study aimed to investigate the effect of mating factor recombination on the development of edible mushroom,and explore the possibility for using self-crossing to breed sporeless/low sporing mushroom strains.[Method] Selecting Agrocybe salicacola strain YAASM0711 as the self-crossing material,six self-crossed combinations were directionally constructed with single spore growth rate as an indicator,to carry out comprehensive analysis of the qualitative traits（color,deformity and growth characteristics）,quantitative traits（shape,yield,size and number of fruit bodies） and mating types of its self-crossed F1 progenies.[Result] After mating factor recombination,self-crossed single spore progenies showed serious genetic differentiation,the average mycelial growth rate and agronomic traits of inbred progenies were lower than those of the parents.The phenotype in fruiting body formation could be divided into normal fruiting and abnormal fruiting（including abnormal fruiting bodies,sporeless or low sporing fruiting bodies,no fruiting）.To be specific,18% self-crossed strains had superior agronomic traits to the parents,which can be used for selecting predominant strains from self-crossed progeny populations based on different breeding purposes.[Conclusion] The genes controlling mycelial growth rate of single spore were linked with mating factors.The mating factor-recombinant single spore strains could be selected according to the correlation between mating type and growth rate.The fruiting result of self-crossed strains indicated that the genes related to sporeless/low sporing development were recessive.Not only genetic materials harboring development-deficient genes in edible mushroom can be obtained by self-crossing,but also the sporeless/low sporing mutant strains with important application prospects can be bred.
基金This paper was supported by Natural Science Foundation of Fujian Province (B0010020)
文摘Allelochemicals of Chinese-fir root was extracted by technology ofsupercritical CO_2 extraction under orthogonal experiment design, and it was used to analyzeallelopathic activity of Chinese-fir through bioassay of seed germination. The results showed thatas to the available rate of allelochemicals, the pressure and temperature of extraction were themost important factors. The allelochemicals of Chinese-fir root extracted by pure CO_2 and ethanolmixed with CO_2 have different allelopathic activities to seed germination, and the allelochemicalsextracted by ethanol mixed with CO_2 had stronger inhibitory effects on seed germination than thatextracted by pure CO_2.
基金The National Key Technology R&D Program of Chinaduring the 11th Five-Year Plan Period(No.2006BAH02A12)the National High Technology Research and Development Program of China(863 Program)(No.2006AA010101)
文摘In order to improve the design and implementation quality of web service compositions,formal methods are used to model them and certain properties are verified.WCFA （web service interface control flow automata）is used to model web services,especially the control flow and possible interactions with other web services.A web service composition consists of a set of interacting WCFA.The global behavior of web service compositions is captured by NWA（nested word automata）.A variation of the depth-first search algorithm is used to transform a set of WCFA into an NWA.State formulae and call stacks at each node of NWA are computed by a path-sensitive reachability analysis.Safety properties,call stack inspection properties and pre/post-conditions of service invocations are described by assertions.Then verification of these assertions is carried out by an automated SAT tool.
文摘To realize automatic control of automobile transmission performance test stand Methods The automatic control technique of the lubricant temperature,the program- controll- edautomaticshifting of the transmission,the continuous adjusting of revolution speed and load, data-acquisition and data real-time processing were adopted.Results The lubricant temperature was controlled at the set temperature ±2℃.The automatic shifting of the trans- mission is simple,reliable and accurate.The automatic adjusting of load and rotation speed is rapidandaccurate,the torque divergence is ±1N·m,the rotation speed divergence is ±5r/min Conclusion The four kinds of techniques are applied into the automobile transmission perfor- mance test stand successfully. mancetest stand successfully.
基金Projects(51274099,51474106)supported by the National Natural Science Foundation of China
文摘The coal of Anyuan Mine has the characteristic of easy spontaneous combustion. Conventional method is difficult to predict it. Coal samples from this mine were tested in laboratory. The data obtained from laboratory determination were initialized for the value which was defined as "K". The ratio of each index gas and value of "K", and the ratio of combination index gases and value of "K", were analyzed simultaneously. The research results show that for this coal mine, if there is carbon monoxide in the gas sample, the phenomenon of oxidation and temperature rising for coal exists in this mine; if there is C_2H_4 in the gas sample, the temperature of coal perhaps exceeds 130 °C. If the coal temperature is between 35 °C and 130 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(CO)/K mainly; if the temperature of coal is between 130 °C and 300 °C, prediction and forecast for coal spontaneous combustion depend on the value of Φ(C_2H_6)/Φ(C_2H_2) and Φ(C_2H_6)/K. The research results provide experimental basis for the prediction of coal spontaneous combustion in Anyuan coal mine, and have better guidance on safe production of this coal mine.
文摘Signed direct graph (SDG) theory provides algorithms and methods that can be applied directly to chemical process modeling and analysis to validate simulation models, and is a basis for the development of a software environment that can automate the validation activity. This paper is concentrated on the pretreatment of the model validation. We use the validation scenarios and standard sequences generated by well-established SDG model to validate the trends fitted from the simulation model. The results are helpful to find potential problems, assess possible bugs in the simulation model and solve the problem effectively. A case study on a simulation model of boiler is presented to demonstrate the effectiveness of this method.
