Since diarrheagenic E. coli are not identified by common clinical laboratory techniques, we hypothesized that these organisms might be an unrecognized cause of enteritis in children in the U. S. 1327 children with acu...Since diarrheagenic E. coli are not identified by common clinical laboratory techniques, we hypothesized that these organisms might be an unrecognized cause of enteritis in children in the U. S. 1327 children with acute gastroenteritis were identified prospectively by active surveillance in the Emergency Department (ED) and the inpatient units at Cincinnati Children’s Hospital Medical Center. Stool samples were evaluated for diarrheagenic E. coli using a panel of DNA probes and adherence pattern to HEp-2 cells. Stool samples from a reference group of 555 well children were studied for comparison. Gene probe studies, but not HEp-2 cell adherence, demonstrated that enteroaggregative, diffusely adherent and enteropathogenic E. coli were associated with clinical illness. Each was isolated significantly more often from study subjects in the ED than controls. In children < 1 year of age, enteroaggregative E. coli were isolated significantly more often from both inpatients (4.7%, Odds Ratio = 3.4, 95%confidence intervals 1.3-9.1, p < 0.03) and ED patients (10.0%, Odds Ratio = 7.2, 95%con-fidence intervals 2.9-18.2, p < 0.001) than from well children (1.4%). Diarrheagenic E. coli, especially enteroaggregative E. coli, may be an important, unrecognized cause of childhood diarrhea in the U. S.展开更多
文摘为了解内蒙古地区羊屠宰场各屠宰环节致泻性大肠杆菌和沙门氏菌的携带情况,评估是否存在污染风险,选取不同规模屠宰场,用棉拭子法分别采集不同屠宰环节样品349份,36 h内进行试验;应用PCR方法分离鉴定病原菌,对鉴定为致泻性大肠杆菌的分离株进行16S r DNA测序并构建系统发育树。结果显示:从刚宰杀的羊胴体表面和环境中共分离到致泻性大肠杆菌12株,其中11株携带毒力基因elt,1株携带毒力基因eae和elt;大中型屠宰场(1.21%)的致泻性大肠杆菌阳性率低于小型屠宰场(5.43%),不同规模屠宰场的预冷间均未检出致泻性大肠杆菌和沙门氏菌;从屠宰环境中分离出1株携带inv A基因的沙门氏菌,阳性率为0.29%;经高压冲洗和排酸预冷等屠宰工艺后,在羊胴体表面均未发现致泻性大肠杆菌和沙门氏菌。
文摘Since diarrheagenic E. coli are not identified by common clinical laboratory techniques, we hypothesized that these organisms might be an unrecognized cause of enteritis in children in the U. S. 1327 children with acute gastroenteritis were identified prospectively by active surveillance in the Emergency Department (ED) and the inpatient units at Cincinnati Children’s Hospital Medical Center. Stool samples were evaluated for diarrheagenic E. coli using a panel of DNA probes and adherence pattern to HEp-2 cells. Stool samples from a reference group of 555 well children were studied for comparison. Gene probe studies, but not HEp-2 cell adherence, demonstrated that enteroaggregative, diffusely adherent and enteropathogenic E. coli were associated with clinical illness. Each was isolated significantly more often from study subjects in the ED than controls. In children < 1 year of age, enteroaggregative E. coli were isolated significantly more often from both inpatients (4.7%, Odds Ratio = 3.4, 95%confidence intervals 1.3-9.1, p < 0.03) and ED patients (10.0%, Odds Ratio = 7.2, 95%con-fidence intervals 2.9-18.2, p < 0.001) than from well children (1.4%). Diarrheagenic E. coli, especially enteroaggregative E. coli, may be an important, unrecognized cause of childhood diarrhea in the U. S.
