Ovarian cancer is a major health problem for women in the United States. Despite evidence of considerable heterogeneity, most cases of ovarian cancer are treated in a similar fashion. The molecular basis for the clini...Ovarian cancer is a major health problem for women in the United States. Despite evidence of considerable heterogeneity, most cases of ovarian cancer are treated in a similar fashion. The molecular basis for the clinicopathologic characteristics of these tumors remains poorly defined. Whole genome expression profiling is a genomic tool, which can identify dysregulated genes and uncover unique sub-classes of tumors. The application of this technology to ovarian cancer has provided a solid molecular basis for differences in histology and grade of ovarian tumors. Differentially expressed genes identified pathways implicated in cell proliferation, invasion, motility, chromosomal instability, and gene silencing and provided new insights into the origin and potential treatment of these cancers. The added knowledge provided by global gene expression profiling should allow for a more rational treatment of ovarian cancers. These techniques are leading to a paradigm shift from empirical treatment to an individually tailored approach. This review summarizes the new genomic data on epithelial ovarian cancers of different histology and grade and the impact it will have on our understanding and treatment of this disease.展开更多
To support the scientific basis for rapid identification of pathogenic bacteria and other stud- ies,the sequences of hsp60 gene in major 34 species of 16 genus of pathogenic bacteria were search out in GenBank and a p...To support the scientific basis for rapid identification of pathogenic bacteria and other stud- ies,the sequences of hsp60 gene in major 34 species of 16 genus of pathogenic bacteria were search out in GenBank and a proper pair of universal degenerate primer was designed by means of the molecu- lar biological softwawe Primer 5.0 and Oligo 6.0.This primer was then used in the PCR amplification, and the hsp60 gene fragments of the selected pathogenic bacteria could be amplified using this degener- ate primer.By way of bioinformational analysis,the conservation,variation and the interspecies phylo- genetic relations of the hsp60 gene sequence were analysed.From the results of the comparative study on sequences,it was demonstrated that the hsp60 gene was characterized by conservation and varia- tion,in which the conserved and mutant regions co-existed and separately distributed with many small mutant regions distributed among the conserved regions,just like the mosaic.The phylogenetic tree among different pathogenic bacteria drawn from the hsp60 gene analysis was proved to be consistent with those from 16S rRNA and 23S rRNA.It is concluded that the sequence distribution of hsp60 gene would provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.展开更多
文摘Ovarian cancer is a major health problem for women in the United States. Despite evidence of considerable heterogeneity, most cases of ovarian cancer are treated in a similar fashion. The molecular basis for the clinicopathologic characteristics of these tumors remains poorly defined. Whole genome expression profiling is a genomic tool, which can identify dysregulated genes and uncover unique sub-classes of tumors. The application of this technology to ovarian cancer has provided a solid molecular basis for differences in histology and grade of ovarian tumors. Differentially expressed genes identified pathways implicated in cell proliferation, invasion, motility, chromosomal instability, and gene silencing and provided new insights into the origin and potential treatment of these cancers. The added knowledge provided by global gene expression profiling should allow for a more rational treatment of ovarian cancers. These techniques are leading to a paradigm shift from empirical treatment to an individually tailored approach. This review summarizes the new genomic data on epithelial ovarian cancers of different histology and grade and the impact it will have on our understanding and treatment of this disease.
文摘To support the scientific basis for rapid identification of pathogenic bacteria and other stud- ies,the sequences of hsp60 gene in major 34 species of 16 genus of pathogenic bacteria were search out in GenBank and a proper pair of universal degenerate primer was designed by means of the molecu- lar biological softwawe Primer 5.0 and Oligo 6.0.This primer was then used in the PCR amplification, and the hsp60 gene fragments of the selected pathogenic bacteria could be amplified using this degener- ate primer.By way of bioinformational analysis,the conservation,variation and the interspecies phylo- genetic relations of the hsp60 gene sequence were analysed.From the results of the comparative study on sequences,it was demonstrated that the hsp60 gene was characterized by conservation and varia- tion,in which the conserved and mutant regions co-existed and separately distributed with many small mutant regions distributed among the conserved regions,just like the mosaic.The phylogenetic tree among different pathogenic bacteria drawn from the hsp60 gene analysis was proved to be consistent with those from 16S rRNA and 23S rRNA.It is concluded that the sequence distribution of hsp60 gene would provide a solid basis for the rapid identification of pathogenic bacteria and the development of a diagnostic microarray.
