为了明确忍冬根腐病的发生规律,以从忍冬根腐病株病根分离到的2种致病镰刀菌菌株FQ3(Fusarium solani species complex)、FQ4(Fusarium incarnatum-equiseti species complex)为研究对象,考察了其生物学特性。结果表明,菌株FQ3最适生长...为了明确忍冬根腐病的发生规律,以从忍冬根腐病株病根分离到的2种致病镰刀菌菌株FQ3(Fusarium solani species complex)、FQ4(Fusarium incarnatum-equiseti species complex)为研究对象,考察了其生物学特性。结果表明,菌株FQ3最适生长温度为30℃,最适产孢温度为35℃,最适生长pH值为9,最适产孢pH值为7～10,全黑暗条件最有利于菌丝生长和产孢,生长最适碳、氮源分别为乳糖和甘氨酸,产孢最适碳、氮源分别为葡萄糖和硝酸钠;菌株FQ4最适生长温度为25～30℃,最适产孢温度为30℃,最适生长pH值为7～10,最适产孢pH值为9,半光照条件有利于其生长和产孢,生长最适碳、氮源分别为蔗糖和酵母,产孢最适碳氮源分别为果糖和酵母。除温度处理外,菌株FQ3在所有参试条件下,菌丝生长速度及产孢量均明显高于菌株FQ4。展开更多
The effects of solarisation using clear, UV stabilized, 25μm low density polyethylene mulching combined with soil amendment of chicken manures 12 th^-1, mixed fungicides of Metalaxyl 2 g-Benlate 1.5 g L^-l, Biocontro...The effects of solarisation using clear, UV stabilized, 25μm low density polyethylene mulching combined with soil amendment of chicken manures 12 th^-1, mixed fungicides of Metalaxyl 2 g-Benlate 1.5 g L^-l, Biocontrol agent of Trichoderma harzianum （T.h.） and NPK fertilizer 180 Kg h^-1, on the survived micro and macrocondia of Fusarium proliferatum （Matsushita） Nirenberg were ascertained during summer 2008. Mulched treatments within 45 and 60 days significantly reduced viable propagules to 6 and 3.4 × l03 cfu1 g soil, respectively at 5 cm depth followed by 15 and 30 cm soil depth. Natural heating of dry soil reduced an initial population of 76 × 10^3 to 46.73, 49 and 49.13 × 103 cfu＇lg soil at 5, 15 and 30 cm depth, respectively. Therefore, Fusarium proliferatum reduced by 85.29 and 89.22% within 45 and 60 days compared to 38% in control aired soil. Application of mulching with T. harzianum caused a worthwhile reduction 84.37% in viable propagules, solar with low doses of Metalaxyl-Benlate further reduced Fusarium propagules at all depths 95.5%. Combining chicken manures proved its reduction of fungi cfu at 5 cm depth only, whereas NPK amendments failed in their reduction effects at various depths. However, almost 95% reduction in Fusarium propagules was achieved at various soil depths when combining Met.-Ben., with soil mulching. Combining T.h. was also proved to be significant in reducing fungus inoculum by 88.58, 96.06% at 5 and 15 cm depth. Generally, manures and NPK fertilizers amended soil found equally effective in reducing cfu of Fusarium at 5 and 15 cm depth similar to polyethylene mulching for both solar duration.展开更多
文摘为了明确忍冬根腐病的发生规律,以从忍冬根腐病株病根分离到的2种致病镰刀菌菌株FQ3(Fusarium solani species complex)、FQ4(Fusarium incarnatum-equiseti species complex)为研究对象,考察了其生物学特性。结果表明,菌株FQ3最适生长温度为30℃,最适产孢温度为35℃,最适生长pH值为9,最适产孢pH值为7～10,全黑暗条件最有利于菌丝生长和产孢,生长最适碳、氮源分别为乳糖和甘氨酸,产孢最适碳、氮源分别为葡萄糖和硝酸钠;菌株FQ4最适生长温度为25～30℃,最适产孢温度为30℃,最适生长pH值为7～10,最适产孢pH值为9,半光照条件有利于其生长和产孢,生长最适碳、氮源分别为蔗糖和酵母,产孢最适碳氮源分别为果糖和酵母。除温度处理外,菌株FQ3在所有参试条件下,菌丝生长速度及产孢量均明显高于菌株FQ4。
文摘The effects of solarisation using clear, UV stabilized, 25μm low density polyethylene mulching combined with soil amendment of chicken manures 12 th^-1, mixed fungicides of Metalaxyl 2 g-Benlate 1.5 g L^-l, Biocontrol agent of Trichoderma harzianum （T.h.） and NPK fertilizer 180 Kg h^-1, on the survived micro and macrocondia of Fusarium proliferatum （Matsushita） Nirenberg were ascertained during summer 2008. Mulched treatments within 45 and 60 days significantly reduced viable propagules to 6 and 3.4 × l03 cfu1 g soil, respectively at 5 cm depth followed by 15 and 30 cm soil depth. Natural heating of dry soil reduced an initial population of 76 × 10^3 to 46.73, 49 and 49.13 × 103 cfu＇lg soil at 5, 15 and 30 cm depth, respectively. Therefore, Fusarium proliferatum reduced by 85.29 and 89.22% within 45 and 60 days compared to 38% in control aired soil. Application of mulching with T. harzianum caused a worthwhile reduction 84.37% in viable propagules, solar with low doses of Metalaxyl-Benlate further reduced Fusarium propagules at all depths 95.5%. Combining chicken manures proved its reduction of fungi cfu at 5 cm depth only, whereas NPK amendments failed in their reduction effects at various depths. However, almost 95% reduction in Fusarium propagules was achieved at various soil depths when combining Met.-Ben., with soil mulching. Combining T.h. was also proved to be significant in reducing fungus inoculum by 88.58, 96.06% at 5 and 15 cm depth. Generally, manures and NPK fertilizers amended soil found equally effective in reducing cfu of Fusarium at 5 and 15 cm depth similar to polyethylene mulching for both solar duration.
