舒铂的合成工艺研究

本文对抗肿瘤新药苏铂的合成工艺进行了研究,采用顺式-二碘-(4R,5R)-4,5-双(氨甲基)-2-异丙基-1,3-二氧戊环烷合铂(II)为起始原料,先后与硫酸银和氢氧化钡复分解反应生成顺式-二羟基-(4R,5R)-4,5-双(氨甲基)-2-异丙基-1,3-二氧戊环烷合铂(II),再与丙二酸反应生成目标化合物,该方法的产率在70%左右,操作简单,适合扩大规模生产。

新型铂类化合物LLC-0601与顺铂、舒铂体外对L-02、HK-2细胞的毒性比较研究

目的比较新型铂类化合物LLC-0601与舒铂、顺铂体外对人胚胎肝细胞(L-02)和人肾小管上皮细胞(HK-2)的毒性。方法采用不同浓度的LLC-0601、顺铂、舒铂分别与L-02细胞和HK-2细胞作用48 h后,四甲基偶氮唑盐(MTT)法检测并计算细胞增殖抑制率,计算IC50;取细胞培养液全自动生化分析仪检测乳酸脱氢酶(LDH)漏出量;显微镜下观察细胞形态改变。结果 LLC-0601、顺铂、舒铂作用两株细胞48 h后,同等剂量下LLC-0601对L-02细胞和HK-2细胞的抑制率都是最低,舒铂次之,顺铂最强,组间比较差异有统计学意义(P<0.05);检测两株细胞培养液中LDH含量,LLC-0601在较高剂量下才出现LDH升高,而顺铂和舒铂在较低剂量下LDH既有明显变化,组间比较差异有统计学意义(P<0.05),其中顺铂又强于舒铂(P<0.05)。3个铂类化合物细胞形态改变均表现随给药浓度增加,出现细胞核空泡样改变,细胞膜不完整,继续增加给药浓度后细胞死亡。结论 LLC-0601体外对L-02和HK-2细胞的毒性明显低于顺铂、舒铂,是一个有开发前景的新化合物。

Skin temperature measurement method

To improve the accuracy of skin temperature measurements in thermal comfort research,a new measurement method based on a new thermometer is proposed.A platinum film resistance（Pt1000）sensor of the thermometer is welded on a printed circuit board to eliminate the heat loss from the leads and avoid the influence of the surrounding thermal environment.In order to determine the suitable thickness of the board,a steady heat conduction model is established.The simulation results reveal that when the thickness of the board is 0.2 mm,the influence of the surrounding air can be effectively prevented and the skin temperature does not obviously increase.The experimental results of verification show that the maximum measurement error of the skin temperature measured by the thermometer is 0.24 ℃,and the average measurement error of the skin temperature is 0.04 ℃.The proposed method provides an effective and reliable option for the skin temperature measurement in thermal comfort research.