In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.A...In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.An improved Intensity-Hue-Saturation(IHS) fusion algorithm is proposed to fuse panchromatic and multispectral images,in which intensity component and panchromatic image are combined using the weights determined by edge pixels in the panchromatic image identified by grey absolute correlation degree.This improved IHS fusion algorithm outper-forms traditional IHS fusion method to a certain extent,evidenced by its ability in preserving spectral information and enhancing spatial details.Dempster-Shafer(D-S) evidence theory was adopted to combine the outputs of three member classifiers to generate the final classification map with higher accuracy than that by any individual classifier.Based on this study,we conclude that Beijing-1 small satellite remote sensing images are useful to monitor and analyze land cover change and ecological environment degradation in mining areas,and the proposed fusion algorithms at data and decision levels can integrate the advantages of multi-resolution images and multiple classifiers,improve the overall accuracy and produce a more reliable land cover map.展开更多
Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel...Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome （BAC） transgenic mouse line that expresses Class Ⅲ β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination, mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class Ⅲ β-tubulin The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.展开更多
基金Under the auspices of National Natural Science Foundation of China (No. 40871195)Opening Fund of Beijing-1Small Satellite Data Applications from State Key Laboratory for Remote Sensing Science (No. 200709)National High Technology Research and Development Program of China (No. 2007AA12Z162)
文摘In order to promote the application of Beijing-1 small satellite(BJ-1) remote sensing data,the multispectral and panchromatic images captured by BJ-1 were used for land cover classification in Pangzhuang Coal Mining.An improved Intensity-Hue-Saturation(IHS) fusion algorithm is proposed to fuse panchromatic and multispectral images,in which intensity component and panchromatic image are combined using the weights determined by edge pixels in the panchromatic image identified by grey absolute correlation degree.This improved IHS fusion algorithm outper-forms traditional IHS fusion method to a certain extent,evidenced by its ability in preserving spectral information and enhancing spatial details.Dempster-Shafer(D-S) evidence theory was adopted to combine the outputs of three member classifiers to generate the final classification map with higher accuracy than that by any individual classifier.Based on this study,we conclude that Beijing-1 small satellite remote sensing images are useful to monitor and analyze land cover change and ecological environment degradation in mining areas,and the proposed fusion algorithms at data and decision levels can integrate the advantages of multi-resolution images and multiple classifiers,improve the overall accuracy and produce a more reliable land cover map.
文摘Class Ⅲ β-tubulin （Tubb3） is a component of the microtubules in neurons and contributes to microtubule dynamics that are required for axon outgrowth and guidance during neuronal development. We here report a novel bacterial artificial chromosome （BAC） transgenic mouse line that expresses Class Ⅲ β-tubulin fused to mCherry, an improved monomeric red fluorescent protein, for the visualization of microtubules during neuronal development. A BAC containing Tubb3 gene was modified by insertion of mCherry complementary DNA downstream of Tubb3 coding sequence via homologous recombination, mCherry fusion protein was expressed in the nervous system and testis of the transgenic animal, and the fluorescent signal was observed in the neurons that located in the olfactory bulb, cerebral cortex, hippocampal formation, cerebellum, as well as the retina. Besides, Tubb3-mCherry fusion protein mainly distributed in neurites and colocalized with endogenous Class Ⅲ β-tubulin The fusion protein labels Purkinje cell dendrites during cerebellar circuit formation. Therefore, this transgenic line might be a novel tool for scientific community to study neuronal development both in vitro and in vivo.
